Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts: Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism

J E  HESKETH; Gillian Patricia Campbell

doi:10.1007/BF01119769

Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts: Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism

J E HESKETH, Gillian Patricia Campbell

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to
stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin.
Cholera toxin on the other hand stimulated protein synthesis and this effect was
insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol
dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and
transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in
diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 #M) stimulated
protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal
activity through a signal mechanism that involves a G-protein mediated activation of
phospholipase C to increase DAG levels.

Original language	English
Pages (from-to)	533-541
Number of pages	9
Journal	Bioscience Reports
Volume	7
Issue number	7
DOIs	https://doi.org/10.1007/BF01119769
Publication status	Published - Jul 1987

Keywords

insulin
protein synthesis
G-protein
diacylglycerol
phospholipase C
signal transduction

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Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts : Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism . / HESKETH, J E ; Campbell, Gillian Patricia.

In: Bioscience Reports, Vol. 7, No. 7, 07.1987, p. 533-541.

Research output: Contribution to journal › Article › peer-review

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abstract = "The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin. Cholera toxin on the other hand stimulated protein synthesis and this effect was insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 #M) stimulated protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal activity through a signal mechanism that involves a G-protein mediated activation of phospholipase C to increase DAG levels.",

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AB - The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin. Cholera toxin on the other hand stimulated protein synthesis and this effect was insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 #M) stimulated protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal activity through a signal mechanism that involves a G-protein mediated activation of phospholipase C to increase DAG levels.

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