Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts

Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism

J E HESKETH, Gillian Patricia Campbell

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to
    stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin.
    Cholera toxin on the other hand stimulated protein synthesis and this effect was
    insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol
    dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and
    transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in
    diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 #M) stimulated
    protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal
    activity through a signal mechanism that involves a G-protein mediated activation of
    phospholipase C to increase DAG levels.
    Original languageEnglish
    Pages (from-to)533-541
    Number of pages9
    JournalBioscience Reports
    Volume7
    Issue number7
    DOIs
    Publication statusPublished - Jul 1987

    Keywords

    • insulin
    • protein synthesis
    • G-protein
    • diacylglycerol
    • phospholipase C
    • signal transduction

    Cite this

    @article{7550a9b5336541ae8bcdebe4e582e145,
    title = "Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts: Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism",
    abstract = "The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin. Cholera toxin on the other hand stimulated protein synthesis and this effect was insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 #M) stimulated protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal activity through a signal mechanism that involves a G-protein mediated activation of phospholipase C to increase DAG levels.",
    keywords = "insulin, protein synthesis, G-protein, diacylglycerol, phospholipase C, signal transduction",
    author = "HESKETH, {J E} and Campbell, {Gillian Patricia}",
    year = "1987",
    month = "7",
    doi = "10.1007/BF01119769",
    language = "English",
    volume = "7",
    pages = "533--541",
    journal = "Bioscience Reports",
    issn = "0144-8463",
    publisher = "Portland Press Ltd.",
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    TY - JOUR

    T1 - Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts

    T2 - Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism

    AU - HESKETH, J E

    AU - Campbell, Gillian Patricia

    PY - 1987/7

    Y1 - 1987/7

    N2 - The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin. Cholera toxin on the other hand stimulated protein synthesis and this effect was insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 #M) stimulated protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal activity through a signal mechanism that involves a G-protein mediated activation of phospholipase C to increase DAG levels.

    AB - The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin. Cholera toxin on the other hand stimulated protein synthesis and this effect was insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 #M) stimulated protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal activity through a signal mechanism that involves a G-protein mediated activation of phospholipase C to increase DAG levels.

    KW - insulin

    KW - protein synthesis

    KW - G-protein

    KW - diacylglycerol

    KW - phospholipase C

    KW - signal transduction

    U2 - 10.1007/BF01119769

    DO - 10.1007/BF01119769

    M3 - Article

    VL - 7

    SP - 533

    EP - 541

    JO - Bioscience Reports

    JF - Bioscience Reports

    SN - 0144-8463

    IS - 7

    ER -