Effects of moderate and high intensity isocaloric aerobic training upon microvascular reactivity and myocardial oxidative stress in rats

Lorena Paes, Daniel Lima, Cristiane Matsuura, Maria das Graças de Souza, Fátima Cyrino, Carolina Barbosa, Fernanda Ferrão, Daniel Bottino, Eliete Bouskela, Paulo Farinatti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Systemic and central cardiovascular adaptations may vary in response to chronic exercise performed with different intensities and volumes. This study compared the effects of aerobic training with different intensities but equivalent volume upon microvascular reactivity in cremaster muscle and myocardial biomarkers of oxidative stress in Wistar rats. After peak oxygen uptake (VO2peak) assessment, rats (n = 24) were assigned into three groups: moderate-intensity exercise training (MI); high-intensity exercise training (HI); sedentary control (SC). Treadmill training occurred during 4 weeks, with exercise bouts matched by the energy expenditure (3.0–3.5 Kcal). Microvascular reactivity was assessed in vivo by intravital microscopy in cremaster muscle arterioles, while biomarkers of oxidative stress and eNOS expression were quantified at left ventricle and at aorta, respectively. Similar increasing vs. sedentary control group (SC) occurred in moderate intensity training group (MI) and high-intensity training group (HI) for endothelium-dependent vasodilation (10-4M: MI: 168.7%, HI: 164.6% vs. SC: 146.6%, P = 0.0004). Superoxide dismutase (SOD) (HI: 0.13 U/mg vs. MI: 0.09 U/mg and SC: 0.06 U/mg; P = 0.02), glutathione peroxidase (GPX) (HI: 0.00038 U/mg vs. MI: 0.00034 U/mg and SC: 0.00024 U/mg; P = 0.04), and carbonyl protein content (HI: 0.04 U/mg vs. MI: 0.03 U/mg and SC: 0.01 U/mg; P = 0.003) increased only in HI. No difference across groups was detected for catalase (CAT) (P = 0.12), Thiobarbituric acid reactive substances (TBARS) (P = 0.38) or eNOS expression in aorta (P = 0.44). In conclusion, higher exercise intensity induced greater improvements in myocardium antioxidant defenses, while gains in microvascular reactivity appeared to rely more on exercise volume than intensity.

Original languageEnglish
Article numbere0218228
Number of pages15
JournalPloS ONE
Volume15
Issue number2
DOIs
Publication statusPublished - 7 Feb 2020

Keywords

  • Animals
  • Aorta/metabolism
  • Exercise Therapy/methods
  • Glutathione Peroxidase/genetics
  • Heart Ventricles/metabolism
  • Male
  • Microvessels/physiology
  • Myocardial Ischemia/therapy
  • Nitric Oxide Synthase Type III/metabolism
  • Oxidative Stress
  • Oxygen Consumption
  • Physical Conditioning, Animal/methods
  • Protein Carbonylation
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase/genetics
  • Vasodilation

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