Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 2320-2327 |
| Number of pages | 8 |
| Journal | Journal of Investigative Dermatology |
| Volume | 130 |
| Issue number | 9 |
| Early online date | 22 Apr 2010 |
| DOIs | |
| Publication status | Published - Sep 2010 |
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Effects of physiological electric fields on migration of human dermal fibroblasts. / Guo, Aihua; Song, Bing; Reid, Brian; Gu, Yu; Forrester, John; Jahoda, Colin A B; Zhao, Min.
In: Journal of Investigative Dermatology, Vol. 130, No. 9, 09.2010, p. 2320-2327.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Effects of physiological electric fields on migration of human dermal fibroblasts
AU - Guo, Aihua
AU - Song, Bing
AU - Reid, Brian
AU - Gu, Yu
AU - Forrester, John
AU - Jahoda, Colin A B
AU - Zhao, Min
PY - 2010/9
Y1 - 2010/9
N2 - Endogenous electric currents generated instantly at skin wounds direct migration of epithelial cells and are likely to be important in wound healing. Migration of fibroblasts is critical in wound healing. It remains unclear how wound electric fields guide migration of dermal fibroblasts. We report here that mouse skin wounds generated endogenous electric currents for many hours. Human dermal fibroblasts of both primary and cell-line cultures migrated directionally but slowly toward the anode in an electric field of 50-100 mV mm(-1). This is different from keratinocytes, which migrate quickly to the cathode. It took more than 1 hour for dermal fibroblasts to manifest detectable directional migration. Larger field strength (400 mV mm(-1)) was required to induce directional migration within 1 hour after onset of the field. Phosphatidylinositol-3-OH kinase (PI3 kinase) mediates cathode-directed migration of keratinocytes. We tested the role of PI3 kinase in anode-directed migration of fibroblasts. An applied electric field activated PI3 kinase/Akt in dermal fibroblasts. Dermal fibroblasts from p110gamma (a PI3 kinase catalytic subunit) null mice showed significantly decreased directional migration. These results suggest that physiological electric fields may regulate motility of dermal fibroblasts and keratinocytes differently, albeit using similar PI3 kinase-dependent mechanisms.Journal of Investigative Dermatology advance online publication, 22 April 2010; doi:10.1038/jid.2010.96.
AB - Endogenous electric currents generated instantly at skin wounds direct migration of epithelial cells and are likely to be important in wound healing. Migration of fibroblasts is critical in wound healing. It remains unclear how wound electric fields guide migration of dermal fibroblasts. We report here that mouse skin wounds generated endogenous electric currents for many hours. Human dermal fibroblasts of both primary and cell-line cultures migrated directionally but slowly toward the anode in an electric field of 50-100 mV mm(-1). This is different from keratinocytes, which migrate quickly to the cathode. It took more than 1 hour for dermal fibroblasts to manifest detectable directional migration. Larger field strength (400 mV mm(-1)) was required to induce directional migration within 1 hour after onset of the field. Phosphatidylinositol-3-OH kinase (PI3 kinase) mediates cathode-directed migration of keratinocytes. We tested the role of PI3 kinase in anode-directed migration of fibroblasts. An applied electric field activated PI3 kinase/Akt in dermal fibroblasts. Dermal fibroblasts from p110gamma (a PI3 kinase catalytic subunit) null mice showed significantly decreased directional migration. These results suggest that physiological electric fields may regulate motility of dermal fibroblasts and keratinocytes differently, albeit using similar PI3 kinase-dependent mechanisms.Journal of Investigative Dermatology advance online publication, 22 April 2010; doi:10.1038/jid.2010.96.
U2 - 10.1038/jid.2010.96
DO - 10.1038/jid.2010.96
M3 - Article
C2 - 20410911
VL - 130
SP - 2320
EP - 2327
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 9
ER -