Effects of phytoestrogens on growth and DNA integrity in human prostate tumor cell lines

J H Mitchell, Susan Joyce Duthie, A R Collins

    Research output: Contribution to journalArticle

    58 Citations (Scopus)

    Abstract

    Prostate cancer is one of the most common male cancers in Western countries, yet the incidence of this fatal disease remains low in Asian populations. Environmental factors such as diet play an important role in hormone-dependent cancer etiology, and a high phytoestrogen intake may be one factor contributing to the low prostate cancer mortality in Eastern populations. In this study, we investigated the effects of the phytoestrogens genistein, daidzein, coumestrol, and equol on cell growth and DNA damage (strand breakage) in two human prostate tumor cell lines: androgen receptor positive LNCaP and androgen receptor-negative PC-3. Each compound caused growth inhibition at physiologically relevant concentrations (< 10 muM). Genistein induced DNA damage in both cell lines at < 10 muM. Daidzein inhibited cell growth at 10-100 muM yet had no effect on DNA damage at up to 500 muM. Thus, despite their structural similarities, different phytoestrogens inhibit prostate tumor cell growth by independent mechanisms.

    Original languageEnglish
    Pages (from-to)223-228
    Number of pages6
    JournalNutrition and Cancer
    Volume38
    Issue number2
    DOIs
    Publication statusPublished - 2000

    Keywords

    • ISOFLAVONE GENISTEIN
    • CANCER CELLS
    • LIGNANS
    • ESTROGENS
    • PLASMA
    • MEN
    • ANTIOXIDANT
    • MECHANISMS
    • INHIBITION
    • JAPANESE

    Cite this

    Effects of phytoestrogens on growth and DNA integrity in human prostate tumor cell lines. / Mitchell, J H ; Duthie, Susan Joyce; Collins, A R .

    In: Nutrition and Cancer, Vol. 38, No. 2, 2000, p. 223-228.

    Research output: Contribution to journalArticle

    Mitchell, J H ; Duthie, Susan Joyce ; Collins, A R . / Effects of phytoestrogens on growth and DNA integrity in human prostate tumor cell lines. In: Nutrition and Cancer. 2000 ; Vol. 38, No. 2. pp. 223-228.
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    KW - ESTROGENS

    KW - PLASMA

    KW - MEN

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    KW - MECHANISMS

    KW - INHIBITION

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