EFFECTS OF POST-OVULATION NUTRITIONAL-STATUS IN EWES ON EARLY CONCEPTUS SURVIVAL AND GROWTH IN-VIVO AND LUTEOTROPHIC PROTEIN SECRETION IN-VITRO

Jacqueline Wallace, Raymond Aitken, M A CHEYNE

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Overfeeding during early pregnancy in ewes compromises pregnancy establishment and/or embryo survival. To determine whether high feed intakes after ovulation alter the secretory dialogue between the conceptus and the endometrium, 24 embryos (8-16-cell) from ewes fed maintenance rations were synchronously transferred in singleton on Day 3 of the cycle (oestrus, Day 0) into the uterus of ewes receiving a high or low plane of nutrition from Day 0 (n = 12 ewes per group). Embryo survival and conceptus growth were assessed on Day 16. At this time, pregnancy was maintained in 11 of 12 recipient ewes per group and conceptus mass was not influenced by nutritional plane (637+/-48 v. 583+/-72 mg for high and low groups respectively). Conceptus and endometrial tissues were cultured separately for a further 24 h in vitro in the presence of [H-3]leucine. There was no quantitative difference between nutritional treatments in the incorporation of radiolabel into proteins synthesized and secreted by the conceptus or endometrium. Secretion of ovine trophoblast protein-1 was also similar in both groups. Peripheral progesterone concentrations were significantly (P < 0.05) lower throughout the luteal phase in recipient ewes on high v. low intakes after ovulation. This effect was independent of ovulation rate which was 3.1+/-0.40 and 2.6+/-0.25 corpora lutea for high and low groups respectively.

A high plane of nutrition after ovulation did not influence embryo survival and development in vivo or luteotrophic protein secretion in vitro despite a reduction in peripheral progesterone concentrations. These results imply that, if a high feed intake affects embryo survival, either it has to reduce progesterone concentrations below those measured in the current study or it acts before the embryo enters the uterus or after the embryo has successfully overcome luteolysis on Day 16 of the cycle.

Original languageEnglish
Pages (from-to)253-259
Number of pages7
JournalReproduction, Fertility and Development
Volume6
Issue number2
Publication statusPublished - 1994

Keywords

  • NUTRITION
  • PREGNANCY
  • EMBRYO SURVIVAL
  • PROTEIN
  • ENDOMETRIUM
  • ENDOMETRIAL FUNCTION
  • EARLY-PREGNANCY
  • PROGESTERONE
  • SHEEP
  • FERTILITY
  • GILTS

Cite this

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title = "EFFECTS OF POST-OVULATION NUTRITIONAL-STATUS IN EWES ON EARLY CONCEPTUS SURVIVAL AND GROWTH IN-VIVO AND LUTEOTROPHIC PROTEIN SECRETION IN-VITRO",
abstract = "Overfeeding during early pregnancy in ewes compromises pregnancy establishment and/or embryo survival. To determine whether high feed intakes after ovulation alter the secretory dialogue between the conceptus and the endometrium, 24 embryos (8-16-cell) from ewes fed maintenance rations were synchronously transferred in singleton on Day 3 of the cycle (oestrus, Day 0) into the uterus of ewes receiving a high or low plane of nutrition from Day 0 (n = 12 ewes per group). Embryo survival and conceptus growth were assessed on Day 16. At this time, pregnancy was maintained in 11 of 12 recipient ewes per group and conceptus mass was not influenced by nutritional plane (637+/-48 v. 583+/-72 mg for high and low groups respectively). Conceptus and endometrial tissues were cultured separately for a further 24 h in vitro in the presence of [H-3]leucine. There was no quantitative difference between nutritional treatments in the incorporation of radiolabel into proteins synthesized and secreted by the conceptus or endometrium. Secretion of ovine trophoblast protein-1 was also similar in both groups. Peripheral progesterone concentrations were significantly (P < 0.05) lower throughout the luteal phase in recipient ewes on high v. low intakes after ovulation. This effect was independent of ovulation rate which was 3.1+/-0.40 and 2.6+/-0.25 corpora lutea for high and low groups respectively.A high plane of nutrition after ovulation did not influence embryo survival and development in vivo or luteotrophic protein secretion in vitro despite a reduction in peripheral progesterone concentrations. These results imply that, if a high feed intake affects embryo survival, either it has to reduce progesterone concentrations below those measured in the current study or it acts before the embryo enters the uterus or after the embryo has successfully overcome luteolysis on Day 16 of the cycle.",
keywords = "NUTRITION, PREGNANCY, EMBRYO SURVIVAL, PROTEIN, ENDOMETRIUM, ENDOMETRIAL FUNCTION, EARLY-PREGNANCY, PROGESTERONE, SHEEP, FERTILITY, GILTS",
author = "Jacqueline Wallace and Raymond Aitken and CHEYNE, {M A}",
year = "1994",
language = "English",
volume = "6",
pages = "253--259",
journal = "Reproduction, Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO",
number = "2",

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TY - JOUR

T1 - EFFECTS OF POST-OVULATION NUTRITIONAL-STATUS IN EWES ON EARLY CONCEPTUS SURVIVAL AND GROWTH IN-VIVO AND LUTEOTROPHIC PROTEIN SECRETION IN-VITRO

AU - Wallace, Jacqueline

AU - Aitken, Raymond

AU - CHEYNE, M A

PY - 1994

Y1 - 1994

N2 - Overfeeding during early pregnancy in ewes compromises pregnancy establishment and/or embryo survival. To determine whether high feed intakes after ovulation alter the secretory dialogue between the conceptus and the endometrium, 24 embryos (8-16-cell) from ewes fed maintenance rations were synchronously transferred in singleton on Day 3 of the cycle (oestrus, Day 0) into the uterus of ewes receiving a high or low plane of nutrition from Day 0 (n = 12 ewes per group). Embryo survival and conceptus growth were assessed on Day 16. At this time, pregnancy was maintained in 11 of 12 recipient ewes per group and conceptus mass was not influenced by nutritional plane (637+/-48 v. 583+/-72 mg for high and low groups respectively). Conceptus and endometrial tissues were cultured separately for a further 24 h in vitro in the presence of [H-3]leucine. There was no quantitative difference between nutritional treatments in the incorporation of radiolabel into proteins synthesized and secreted by the conceptus or endometrium. Secretion of ovine trophoblast protein-1 was also similar in both groups. Peripheral progesterone concentrations were significantly (P < 0.05) lower throughout the luteal phase in recipient ewes on high v. low intakes after ovulation. This effect was independent of ovulation rate which was 3.1+/-0.40 and 2.6+/-0.25 corpora lutea for high and low groups respectively.A high plane of nutrition after ovulation did not influence embryo survival and development in vivo or luteotrophic protein secretion in vitro despite a reduction in peripheral progesterone concentrations. These results imply that, if a high feed intake affects embryo survival, either it has to reduce progesterone concentrations below those measured in the current study or it acts before the embryo enters the uterus or after the embryo has successfully overcome luteolysis on Day 16 of the cycle.

AB - Overfeeding during early pregnancy in ewes compromises pregnancy establishment and/or embryo survival. To determine whether high feed intakes after ovulation alter the secretory dialogue between the conceptus and the endometrium, 24 embryos (8-16-cell) from ewes fed maintenance rations were synchronously transferred in singleton on Day 3 of the cycle (oestrus, Day 0) into the uterus of ewes receiving a high or low plane of nutrition from Day 0 (n = 12 ewes per group). Embryo survival and conceptus growth were assessed on Day 16. At this time, pregnancy was maintained in 11 of 12 recipient ewes per group and conceptus mass was not influenced by nutritional plane (637+/-48 v. 583+/-72 mg for high and low groups respectively). Conceptus and endometrial tissues were cultured separately for a further 24 h in vitro in the presence of [H-3]leucine. There was no quantitative difference between nutritional treatments in the incorporation of radiolabel into proteins synthesized and secreted by the conceptus or endometrium. Secretion of ovine trophoblast protein-1 was also similar in both groups. Peripheral progesterone concentrations were significantly (P < 0.05) lower throughout the luteal phase in recipient ewes on high v. low intakes after ovulation. This effect was independent of ovulation rate which was 3.1+/-0.40 and 2.6+/-0.25 corpora lutea for high and low groups respectively.A high plane of nutrition after ovulation did not influence embryo survival and development in vivo or luteotrophic protein secretion in vitro despite a reduction in peripheral progesterone concentrations. These results imply that, if a high feed intake affects embryo survival, either it has to reduce progesterone concentrations below those measured in the current study or it acts before the embryo enters the uterus or after the embryo has successfully overcome luteolysis on Day 16 of the cycle.

KW - NUTRITION

KW - PREGNANCY

KW - EMBRYO SURVIVAL

KW - PROTEIN

KW - ENDOMETRIUM

KW - ENDOMETRIAL FUNCTION

KW - EARLY-PREGNANCY

KW - PROGESTERONE

KW - SHEEP

KW - FERTILITY

KW - GILTS

M3 - Article

VL - 6

SP - 253

EP - 259

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

SN - 1031-3613

IS - 2

ER -