Effects of Seasonality and a Diet of Brassicas on the Shedding of Escherichia coli o157 in Sheep

Eilidh Margaret Fraser, Marion MacRae, Iain D. Ogden, Ken J. Forbes, Norval James Colin Strachan*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sheep flocks were tested for Escherichia coli O157 from pooled fecal samples while they grazed on pasture in winter, brassicas in spring, and on pasture during the summer. The winter pasture study reported an average individual prevalence of 3.1% (95% confidence interval [CI]: 0.6–5.6%) and an average farm-level prevalence of 10.4% (95% CI: 2.1–18.8%) over the 3-year study period. The spring brassica study reported a prevalence of 0% and the summer pasture study had an individual prevalence of 6.3% (95% CI: 2.1–12.1%) and a farm prevalence of 36.8% (95% CI: 15.8–57.8%). Analysis showed significant differences between the shedding of E. coli O157 in sheep grazing on brassicas in spring when compared to sheep grazing on pasture in the summer (p<0.01) and in winter (p=0.044; odds ratio [OR]=0.106). Furthermore, sheep excreted a lower prevalence of E. coli O157 in winter while grazing on pasture (p=0.017; OR=0.199). E. coli O157 isolates were characterized using polymerase chain reaction for the presence of known virulence factors; all carried the eae and stx2 gene and 10/11 positive flocks possessed the stx2cgene, suggesting that sheep are a potential source of human infection.

Original languageEnglish
Pages (from-to)649-654
Number of pages6
JournalFoodborne Pathogens and Disease
Volume10
Issue number7
Early online date7 May 2013
DOIs
Publication statusPublished - 21 Jul 2013

Fingerprint

Escherichia coli O157
Brassica
Sheep
pastures
Diet
sheep
confidence interval
diet
Confidence Intervals
winter
grazing
odds ratio
summer
flocks
Odds Ratio
farms
Virulence Factors
virulence
polymerase chain reaction
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology
  • Medicine(all)

Cite this

Effects of Seasonality and a Diet of Brassicas on the Shedding of Escherichia coli o157 in Sheep. / Fraser, Eilidh Margaret; MacRae, Marion; Ogden, Iain D.; Forbes, Ken J.; Strachan, Norval James Colin.

In: Foodborne Pathogens and Disease, Vol. 10, No. 7, 21.07.2013, p. 649-654.

Research output: Contribution to journalArticle

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abstract = "Sheep flocks were tested for Escherichia coli O157 from pooled fecal samples while they grazed on pasture in winter, brassicas in spring, and on pasture during the summer. The winter pasture study reported an average individual prevalence of 3.1{\%} (95{\%} confidence interval [CI]: 0.6–5.6{\%}) and an average farm-level prevalence of 10.4{\%} (95{\%} CI: 2.1–18.8{\%}) over the 3-year study period. The spring brassica study reported a prevalence of 0{\%} and the summer pasture study had an individual prevalence of 6.3{\%} (95{\%} CI: 2.1–12.1{\%}) and a farm prevalence of 36.8{\%} (95{\%} CI: 15.8–57.8{\%}). Analysis showed significant differences between the shedding of E. coli O157 in sheep grazing on brassicas in spring when compared to sheep grazing on pasture in the summer (p<0.01) and in winter (p=0.044; odds ratio [OR]=0.106). Furthermore, sheep excreted a lower prevalence of E. coli O157 in winter while grazing on pasture (p=0.017; OR=0.199). E. coli O157 isolates were characterized using polymerase chain reaction for the presence of known virulence factors; all carried the eae and stx2 gene and 10/11 positive flocks possessed the stx2cgene, suggesting that sheep are a potential source of human infection.",
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