Abstract
Population growth, in vitro, of three Paramoeba perurans cultures, one polyclonal (G) and two clonal (B8, CE6, derived from G), previously shown to differ in virulence (B8 > G > CE6), was compared at 10 and 15 °C. B8 showed a significantly higher increase in attached and in suspended amoebae over time at 15 and 10 °C, respectively. CE6 and G also had significantly higher numbers of suspended amoebae at 10 °C compared with 15 °C at experiment termination. However, in contrast to B8, numbers of attached amoebae were significantly higher at 10 °C in CE6 but showed a similar trend in G at the end of the experiment. Numbers of both suspended and attached amoebae were lower in B8 compared with CE6 and G. Significant differences in bacterial community composition and/or relative abundances were found, between cultures, between temperatures and between the same culture with and without amoebae, based on 16S rRNA Illumina MiSeq sequencing. Bacterial diversity was lower in B8 and CE6 compared with G, possibly reflecting selection during clonal isolation. The results indicate that polyclonal P. perurans populations may contain amoebae displaying different growth dynamics. Further studies are required to determine if these differences are linked to differences seen in the bacterial communities.
| Original language | English |
|---|---|
| Pages (from-to) | 533-542 |
| Number of pages | 10 |
| Journal | Parasitology |
| Volume | 146 |
| Issue number | 4 |
| Early online date | 25 Oct 2018 |
| DOIs | |
| Publication status | Published - Apr 2019 |
Bibliographical note
The authors thank Dr Una McCarthy for reading of the manuscript and for invaluable advice regarding the amoebae in vitro experiment, and Dr Graeme Nicol for advice on the microbial communities experiment. The MiSeq data were generated by RTL Genomics, Lubbock, TX, USA.Keywords
- 16S rRNA
- Amoebic gill disease
- Paramoeba perurans
- temperature
- BACTERIA
- NEOPARAMOEBA-PERURANS
- SEQUENCES
- REAL-TIME PCR
- SALAR L.
- FARMED ATLANTIC SALMON
- AMEBIC-GILL-DISEASE
- SEVERITY
- AGD
Access to Document
- Accepted Manuscript
This article has been published in a revised form in Parasitology https://doi.org/10.1017/S0031182018001798. This version is free to view and download for private research and study only. Not for re-distribution, re-sale or use in derivative works. © Cambridge University Press 2018.