Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro

Shahida Shahana; Caroline Kampf; Godfried M. Roomans

doi:10.1080/09622935020138172

Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro

Shahida Shahana, Caroline Kampf, Godfried M. Roomans

Medical Education

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29 Citations (Scopus)

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Abstract

BACKGROUND: Allergic asthma is associated with an increased number of eosinophils in the airway wall. Eosinophils secrete cationic proteins, particularly major basic protein (MBP).

AIM: To investigate the effect of synthetic cationic polypeptides such as poly-L-arginine, which can mimic the effect of MBP, on airway epithelial cells.

METHODS: Cultured airway epithelial cells were exposed to poly-L-arginine, and effects were determined by light and electron microscopy.

RESULTS: Poly-L-arginine induced apoptosis and necrosis. Transmission electron microscopy showed mitochondrial damage and changes in the nucleus. The tight junctions were damaged, as evidenced by penetration of lanthanum. Scanning electron microscopy showed a damaged cell membrane with many pores. Microanalysis showed a significant decrease in the cellular content of magnesium, phosphorus, sodium, potassium and chlorine, and an increase in calcium. Plakoglobin immunoreactivity in the cell membrane was decreased, indicating a decrease in the number of desmosomes

CONCLUSIONS: The results point to poly-L-arginine induced membrane damage, resulting in increased permeability, loss of cell-cell contacts and generalized cell damage.

Original language	English
Pages (from-to)	141-148
Number of pages	8
Journal	Mediators of Inflammation
Volume	11
Issue number	3
DOIs	https://doi.org/10.1080/09622935020138172
Publication status	Published - Jun 2002
Event	Scandinavian Society for Electron Microscopy Meeting - Stockholm, Sweden Duration: 12 Jun 2001 → 15 Jun 2001

Keywords

allergic asthma
eosinophils
major basic protein
poly-t-arginine
epithelial damage

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10.1080/09622935020138172Licence: CC BY

Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro
© 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/3.0/
Final published version, 2.16 MBLicence: CC BY

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Shahana, S., Kampf, C., & Roomans, G. M. (2002). Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro. Mediators of Inflammation, 11(3), 141-148. https://doi.org/10.1080/09622935020138172

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abstract = "BACKGROUND: Allergic asthma is associated with an increased number of eosinophils in the airway wall. Eosinophils secrete cationic proteins, particularly major basic protein (MBP).AIM: To investigate the effect of synthetic cationic polypeptides such as poly-L-arginine, which can mimic the effect of MBP, on airway epithelial cells.METHODS: Cultured airway epithelial cells were exposed to poly-L-arginine, and effects were determined by light and electron microscopy.RESULTS: Poly-L-arginine induced apoptosis and necrosis. Transmission electron microscopy showed mitochondrial damage and changes in the nucleus. The tight junctions were damaged, as evidenced by penetration of lanthanum. Scanning electron microscopy showed a damaged cell membrane with many pores. Microanalysis showed a significant decrease in the cellular content of magnesium, phosphorus, sodium, potassium and chlorine, and an increase in calcium. Plakoglobin immunoreactivity in the cell membrane was decreased, indicating a decrease in the number of desmosomesCONCLUSIONS: The results point to poly-L-arginine induced membrane damage, resulting in increased permeability, loss of cell-cell contacts and generalized cell damage.",

keywords = "allergic asthma, eosinophils, major basic protein, poly-t-arginine, epithelial damage",

author = "Shahida Shahana and Caroline Kampf and Roomans, {Godfried M.}",

note = "The technical assistance of Anders Ahlander, Marianne Ljungkvist and Leif Ljung is gratefully acknowledged. This study was supported financially by the Swedish Allergy and Asthma Foundation and the Swedish Care and Allergy Foundation (V{\aa}rdal).; Scandinavian Society for Electron Microscopy Meeting, SCANDEM 2001 ; Conference date: 12-06-2001 Through 15-06-2001",

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AU - Shahana, Shahida

AU - Kampf, Caroline

AU - Roomans, Godfried M.

N1 - The technical assistance of Anders Ahlander, Marianne Ljungkvist and Leif Ljung is gratefully acknowledged. This study was supported financially by the Swedish Allergy and Asthma Foundation and the Swedish Care and Allergy Foundation (Vårdal).

PY - 2002/6

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N2 - BACKGROUND: Allergic asthma is associated with an increased number of eosinophils in the airway wall. Eosinophils secrete cationic proteins, particularly major basic protein (MBP).AIM: To investigate the effect of synthetic cationic polypeptides such as poly-L-arginine, which can mimic the effect of MBP, on airway epithelial cells.METHODS: Cultured airway epithelial cells were exposed to poly-L-arginine, and effects were determined by light and electron microscopy.RESULTS: Poly-L-arginine induced apoptosis and necrosis. Transmission electron microscopy showed mitochondrial damage and changes in the nucleus. The tight junctions were damaged, as evidenced by penetration of lanthanum. Scanning electron microscopy showed a damaged cell membrane with many pores. Microanalysis showed a significant decrease in the cellular content of magnesium, phosphorus, sodium, potassium and chlorine, and an increase in calcium. Plakoglobin immunoreactivity in the cell membrane was decreased, indicating a decrease in the number of desmosomesCONCLUSIONS: The results point to poly-L-arginine induced membrane damage, resulting in increased permeability, loss of cell-cell contacts and generalized cell damage.

AB - BACKGROUND: Allergic asthma is associated with an increased number of eosinophils in the airway wall. Eosinophils secrete cationic proteins, particularly major basic protein (MBP).AIM: To investigate the effect of synthetic cationic polypeptides such as poly-L-arginine, which can mimic the effect of MBP, on airway epithelial cells.METHODS: Cultured airway epithelial cells were exposed to poly-L-arginine, and effects were determined by light and electron microscopy.RESULTS: Poly-L-arginine induced apoptosis and necrosis. Transmission electron microscopy showed mitochondrial damage and changes in the nucleus. The tight junctions were damaged, as evidenced by penetration of lanthanum. Scanning electron microscopy showed a damaged cell membrane with many pores. Microanalysis showed a significant decrease in the cellular content of magnesium, phosphorus, sodium, potassium and chlorine, and an increase in calcium. Plakoglobin immunoreactivity in the cell membrane was decreased, indicating a decrease in the number of desmosomesCONCLUSIONS: The results point to poly-L-arginine induced membrane damage, resulting in increased permeability, loss of cell-cell contacts and generalized cell damage.

KW - allergic asthma

KW - eosinophils

KW - major basic protein

KW - poly-t-arginine

KW - epithelial damage

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JO - Mediators of Inflammation

JF - Mediators of Inflammation

SN - 0962-9351

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T2 - Scandinavian Society for Electron Microscopy Meeting

Y2 - 12 June 2001 through 15 June 2001

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