Effects of transforming growth factor β1 on rainbow trout Oncorhynchus mykiss macrophage respiratory burst activity

S. I. Jang, L. J. Hardie, C. J. Secombes*

*Corresponding author for this work

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Natural bovine transforming growth factor β1 (TGF-β1) was found to have both enhancing and suppressing effects on the respiratory burst activity of rainbow trout, Oncorhynchus mykiss, head kidney macrophages. Incubation with TGF-β1 alone increased respiratory burst activity using doses from 0.05 to 0.2 ng/mL. However, incubation of activated macrophages with TGF-β1 decreased their respiratory burst activity in a dose-dependent manner. Coincubation of macrophages with activating signals (macrophage activating factor containing supernatants and tumor necrosis factor α) and TGF-β1 inhibited the ability of such signals to increase respiratory burst activity. Similarly, pretreatment of macrophages with TGF-β1 prior to treatment with activating signals resulted in a time and dose-dependent decrease in activity relative to cells not treated with TGF-β1. These suppressive effects were largest using higher doses of TGF-β1 (1 ng/mL). The conservation of TGF-βs and their possible mode of action are discussed.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume18
Issue number4
DOIs
Publication statusPublished - Jul 1994

    Fingerprint

Keywords

  • Deactivation
  • Macrophages
  • Oncorhynchus mykiss
  • Rainbow trout
  • Respiratory burst activity
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this