Effects of two endogenous fatty acid ethanolamides on mouse vasa deferentia

Roger Guy Pertwee, G Griffin, L Hanus, R Mechoulam

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Anandamide (20:3, n-6) (homo-gamma-linolenylethanolamide) and anandamide (22:4, n-6) (7,10,13,16-docosatetraenylethanolamide) are known to be present in porcine brain and to undergo specific binding to cannabinoid binding sites. We have now shown that both compounds inhibit the electrically evoked twitch response of the mouse isolated vas deferens (IC50 = 99.3 and 95.5 nM respectively) indicating that they also have the ability to elicit a response. As electrically evoked contractions of the mouse vas deferens are also inhibited by the endogenous cannabinoid, anandamide (20:4, n - 6) (arachidonylethanolamide; IC50 = 52.7 nM), and by other cannabinoids, we conclude that anandamide (20:3, n - 6) and (22:4, n - 6), may, together with anandamide (20:4, n - 6), serve as endogenous cannabinoid receptor agonists. This conclusion is supported by our other main finding, that vasa deferentia show tolerance to the inhibitory effects of anandamide (20:3, n - 6) and anandamide (22:4, n - 6) when obtained from mice subjected to a Delta(9)-tetrahydrocannabinol pretreatment that is known to induce cannabinoid tolerance.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalEuropean Journal of Pharmacology
Volume259
Issue number2
DOIs
Publication statusPublished - 1 Jul 1994

Keywords

  • CANNABINOID
  • DELTA(9)-TETRAHYDROCANNABINOL
  • ANANDAMIDE
  • CANNABINOID, ENDOGENOUS
  • VAS DEFERENS, MOUSE
  • TOLERANCE
  • CANNABINOID RECEPTOR AGONIST
  • BRAIN CONSTITUENT
  • DELTA-9-TETRAHYDROCANNABINOL
  • BINDS
  • Cannabinoid
  • ¿9-Tetrahydrocannabinol
  • Anandamide
  • endogenous
  • Vas deferens
  • mouse
  • tolerance

Cite this

Effects of two endogenous fatty acid ethanolamides on mouse vasa deferentia . / Pertwee, Roger Guy; Griffin, G ; Hanus, L ; Mechoulam, R .

In: European Journal of Pharmacology, Vol. 259, No. 2, 01.07.1994, p. 115-120.

Research output: Contribution to journalArticle

Pertwee, Roger Guy ; Griffin, G ; Hanus, L ; Mechoulam, R . / Effects of two endogenous fatty acid ethanolamides on mouse vasa deferentia . In: European Journal of Pharmacology. 1994 ; Vol. 259, No. 2. pp. 115-120.
@article{18b61ba527d44f5ca9dbec359b3b60d0,
title = "Effects of two endogenous fatty acid ethanolamides on mouse vasa deferentia",
abstract = "Anandamide (20:3, n-6) (homo-gamma-linolenylethanolamide) and anandamide (22:4, n-6) (7,10,13,16-docosatetraenylethanolamide) are known to be present in porcine brain and to undergo specific binding to cannabinoid binding sites. We have now shown that both compounds inhibit the electrically evoked twitch response of the mouse isolated vas deferens (IC50 = 99.3 and 95.5 nM respectively) indicating that they also have the ability to elicit a response. As electrically evoked contractions of the mouse vas deferens are also inhibited by the endogenous cannabinoid, anandamide (20:4, n - 6) (arachidonylethanolamide; IC50 = 52.7 nM), and by other cannabinoids, we conclude that anandamide (20:3, n - 6) and (22:4, n - 6), may, together with anandamide (20:4, n - 6), serve as endogenous cannabinoid receptor agonists. This conclusion is supported by our other main finding, that vasa deferentia show tolerance to the inhibitory effects of anandamide (20:3, n - 6) and anandamide (22:4, n - 6) when obtained from mice subjected to a Delta(9)-tetrahydrocannabinol pretreatment that is known to induce cannabinoid tolerance.",
keywords = "CANNABINOID, DELTA(9)-TETRAHYDROCANNABINOL, ANANDAMIDE, CANNABINOID, ENDOGENOUS, VAS DEFERENS, MOUSE, TOLERANCE, CANNABINOID RECEPTOR AGONIST, BRAIN CONSTITUENT, DELTA-9-TETRAHYDROCANNABINOL, BINDS, Cannabinoid, ¿9-Tetrahydrocannabinol, Anandamide, endogenous, Vas deferens, mouse, tolerance",
author = "Pertwee, {Roger Guy} and G Griffin and L Hanus and R Mechoulam",
year = "1994",
month = "7",
day = "1",
doi = "10.1016/0014-2999(94)90499-5",
language = "English",
volume = "259",
pages = "115--120",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Effects of two endogenous fatty acid ethanolamides on mouse vasa deferentia

AU - Pertwee, Roger Guy

AU - Griffin, G

AU - Hanus, L

AU - Mechoulam, R

PY - 1994/7/1

Y1 - 1994/7/1

N2 - Anandamide (20:3, n-6) (homo-gamma-linolenylethanolamide) and anandamide (22:4, n-6) (7,10,13,16-docosatetraenylethanolamide) are known to be present in porcine brain and to undergo specific binding to cannabinoid binding sites. We have now shown that both compounds inhibit the electrically evoked twitch response of the mouse isolated vas deferens (IC50 = 99.3 and 95.5 nM respectively) indicating that they also have the ability to elicit a response. As electrically evoked contractions of the mouse vas deferens are also inhibited by the endogenous cannabinoid, anandamide (20:4, n - 6) (arachidonylethanolamide; IC50 = 52.7 nM), and by other cannabinoids, we conclude that anandamide (20:3, n - 6) and (22:4, n - 6), may, together with anandamide (20:4, n - 6), serve as endogenous cannabinoid receptor agonists. This conclusion is supported by our other main finding, that vasa deferentia show tolerance to the inhibitory effects of anandamide (20:3, n - 6) and anandamide (22:4, n - 6) when obtained from mice subjected to a Delta(9)-tetrahydrocannabinol pretreatment that is known to induce cannabinoid tolerance.

AB - Anandamide (20:3, n-6) (homo-gamma-linolenylethanolamide) and anandamide (22:4, n-6) (7,10,13,16-docosatetraenylethanolamide) are known to be present in porcine brain and to undergo specific binding to cannabinoid binding sites. We have now shown that both compounds inhibit the electrically evoked twitch response of the mouse isolated vas deferens (IC50 = 99.3 and 95.5 nM respectively) indicating that they also have the ability to elicit a response. As electrically evoked contractions of the mouse vas deferens are also inhibited by the endogenous cannabinoid, anandamide (20:4, n - 6) (arachidonylethanolamide; IC50 = 52.7 nM), and by other cannabinoids, we conclude that anandamide (20:3, n - 6) and (22:4, n - 6), may, together with anandamide (20:4, n - 6), serve as endogenous cannabinoid receptor agonists. This conclusion is supported by our other main finding, that vasa deferentia show tolerance to the inhibitory effects of anandamide (20:3, n - 6) and anandamide (22:4, n - 6) when obtained from mice subjected to a Delta(9)-tetrahydrocannabinol pretreatment that is known to induce cannabinoid tolerance.

KW - CANNABINOID

KW - DELTA(9)-TETRAHYDROCANNABINOL

KW - ANANDAMIDE

KW - CANNABINOID, ENDOGENOUS

KW - VAS DEFERENS, MOUSE

KW - TOLERANCE

KW - CANNABINOID RECEPTOR AGONIST

KW - BRAIN CONSTITUENT

KW - DELTA-9-TETRAHYDROCANNABINOL

KW - BINDS

KW - Cannabinoid

KW - ¿9-Tetrahydrocannabinol

KW - Anandamide

KW - endogenous

KW - Vas deferens

KW - mouse

KW - tolerance

U2 - 10.1016/0014-2999(94)90499-5

DO - 10.1016/0014-2999(94)90499-5

M3 - Article

VL - 259

SP - 115

EP - 120

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 2

ER -