Electrical Stimulation Directs Migration, Enhances and Orients Cell Division and Upregulates the Chemokine Receptors CXCR4 and CXCR2 in Endothelial Cells

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Abstract

Natural direct current electric fields (DC EFs) within tissues undergoing angiogenesis have the potential to influence vessel formation, but how they affect endothelial cells is not clear. We therefore quantified behaviours of human umbilical vein endothelial cells (HUVEC) and human microvasculature endothelial cells (HMEC) stimulated by EFsin vitro. Both cell types migrated faster and toward the cathode; HUVECs responded to fields as low as 50mV/mm, but the HMEC threshold was 100 mV/mm. Mitosis was stimulated at 50 mV/mm for HMEC and at 150 mV/mm for HUVECs, but the cleavage plane was oriented orthogonal to the field vector at 200 mV/mm for both cell types. That different field strengths induced different cell responses suggests distinct underlying cellular mechanisms. A physiological electric field also upregulated expression of CXCR4 and CXCR2 chemokine receptors and upregulated phosphorylation of both chemokines in HUVEC and HMEC cells. Evidence that DC EFs direct endothelial cell migration, proliferation and upregulate chemokines involved in wound healing suggests a key role for electrical control of capillary production during healing. Our data contribute to the molecular mechanisms by which DC EFs direct endothelial cell behaviour and present a novel signalling paradigm in wound healing, tissue regeneration and angiogenesis-related diseases.

Original languageEnglish
Pages (from-to)39-53
Number of pages15
JournalJournal of Vascular Research
Volume56
Issue number1
Early online date17 Apr 2019
DOIs
Publication statusPublished - May 2019

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Chemokine Receptors
Cell Division
Electric Stimulation
Up-Regulation
Endothelial Cells
Microvessels
Human Umbilical Vein Endothelial Cells
Chemokines
Wound Healing
Interleukin-8B Receptors
Mitosis
Cell Movement
Regeneration
Electrodes
Phosphorylation
Cell Proliferation

Keywords

  • Angiogenesis
  • Wound healing
  • Electric field stimulation
  • Endothelial cells
  • Cell division
  • Chemokine receptor
  • FUNCTIONAL EXPRESSION
  • BREAST-CANCER
  • GROWTH-FACTOR
  • FIELDS
  • DC
  • PROLIFERATION
  • ANGIOGENESIS
  • SIGNALS
  • KERATINOCYTES
  • POTENTIALS

Cite this

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title = "Electrical Stimulation Directs Migration, Enhances and Orients Cell Division and Upregulates the Chemokine Receptors CXCR4 and CXCR2 in Endothelial Cells",
abstract = "Natural direct current electric fields (DC EFs) within tissues undergoing angiogenesis have the potential to influence vessel formation, but how they affect endothelial cells is not clear. We therefore quantified behaviours of human umbilical vein endothelial cells (HUVEC) and human microvasculature endothelial cells (HMEC) stimulated by EFsin vitro. Both cell types migrated faster and toward the cathode; HUVECs responded to fields as low as 50mV/mm, but the HMEC threshold was 100 mV/mm. Mitosis was stimulated at 50 mV/mm for HMEC and at 150 mV/mm for HUVECs, but the cleavage plane was oriented orthogonal to the field vector at 200 mV/mm for both cell types. That different field strengths induced different cell responses suggests distinct underlying cellular mechanisms. A physiological electric field also upregulated expression of CXCR4 and CXCR2 chemokine receptors and upregulated phosphorylation of both chemokines in HUVEC and HMEC cells. Evidence that DC EFs direct endothelial cell migration, proliferation and upregulate chemokines involved in wound healing suggests a key role for electrical control of capillary production during healing. Our data contribute to the molecular mechanisms by which DC EFs direct endothelial cell behaviour and present a novel signalling paradigm in wound healing, tissue regeneration and angiogenesis-related diseases.",
keywords = "Angiogenesis, Wound healing, Electric field stimulation, Endothelial cells, Cell division, Chemokine receptor, FUNCTIONAL EXPRESSION, BREAST-CANCER, GROWTH-FACTOR, FIELDS, DC, PROLIFERATION, ANGIOGENESIS, SIGNALS, KERATINOCYTES, POTENTIALS",
author = "Filipa Cunha and Rajnicek, {Ann M} and McCaig, {Colin D}",
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TY - JOUR

T1 - Electrical Stimulation Directs Migration, Enhances and Orients Cell Division and Upregulates the Chemokine Receptors CXCR4 and CXCR2 in Endothelial Cells

AU - Cunha, Filipa

AU - Rajnicek, Ann M

AU - McCaig, Colin D

N1 - © 2019 S. Karger AG, Basel.

PY - 2019/5

Y1 - 2019/5

N2 - Natural direct current electric fields (DC EFs) within tissues undergoing angiogenesis have the potential to influence vessel formation, but how they affect endothelial cells is not clear. We therefore quantified behaviours of human umbilical vein endothelial cells (HUVEC) and human microvasculature endothelial cells (HMEC) stimulated by EFsin vitro. Both cell types migrated faster and toward the cathode; HUVECs responded to fields as low as 50mV/mm, but the HMEC threshold was 100 mV/mm. Mitosis was stimulated at 50 mV/mm for HMEC and at 150 mV/mm for HUVECs, but the cleavage plane was oriented orthogonal to the field vector at 200 mV/mm for both cell types. That different field strengths induced different cell responses suggests distinct underlying cellular mechanisms. A physiological electric field also upregulated expression of CXCR4 and CXCR2 chemokine receptors and upregulated phosphorylation of both chemokines in HUVEC and HMEC cells. Evidence that DC EFs direct endothelial cell migration, proliferation and upregulate chemokines involved in wound healing suggests a key role for electrical control of capillary production during healing. Our data contribute to the molecular mechanisms by which DC EFs direct endothelial cell behaviour and present a novel signalling paradigm in wound healing, tissue regeneration and angiogenesis-related diseases.

AB - Natural direct current electric fields (DC EFs) within tissues undergoing angiogenesis have the potential to influence vessel formation, but how they affect endothelial cells is not clear. We therefore quantified behaviours of human umbilical vein endothelial cells (HUVEC) and human microvasculature endothelial cells (HMEC) stimulated by EFsin vitro. Both cell types migrated faster and toward the cathode; HUVECs responded to fields as low as 50mV/mm, but the HMEC threshold was 100 mV/mm. Mitosis was stimulated at 50 mV/mm for HMEC and at 150 mV/mm for HUVECs, but the cleavage plane was oriented orthogonal to the field vector at 200 mV/mm for both cell types. That different field strengths induced different cell responses suggests distinct underlying cellular mechanisms. A physiological electric field also upregulated expression of CXCR4 and CXCR2 chemokine receptors and upregulated phosphorylation of both chemokines in HUVEC and HMEC cells. Evidence that DC EFs direct endothelial cell migration, proliferation and upregulate chemokines involved in wound healing suggests a key role for electrical control of capillary production during healing. Our data contribute to the molecular mechanisms by which DC EFs direct endothelial cell behaviour and present a novel signalling paradigm in wound healing, tissue regeneration and angiogenesis-related diseases.

KW - Angiogenesis

KW - Wound healing

KW - Electric field stimulation

KW - Endothelial cells

KW - Cell division

KW - Chemokine receptor

KW - FUNCTIONAL EXPRESSION

KW - BREAST-CANCER

KW - GROWTH-FACTOR

KW - FIELDS

KW - DC

KW - PROLIFERATION

KW - ANGIOGENESIS

KW - SIGNALS

KW - KERATINOCYTES

KW - POTENTIALS

UR - http://www.mendeley.com/research/electrical-stimulation-directs-migration-enhances-orients-cell-division-upregulates-chemokine-recept

U2 - 10.1159/000495311

DO - 10.1159/000495311

M3 - Article

C2 - 30995642

VL - 56

SP - 39

EP - 53

JO - Journal of Vascular Research

JF - Journal of Vascular Research

SN - 1018-1172

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