Elevated rate of collagen solubilization and postmortem degradation in muscles of lambs with high growth rates: Possible relationship with activity of matrix metalloproteinases

M N Sylvestre, Denis Pierre Balcerzak, C Feidt, V E Baracos, J B Bellut

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    The extracellular matrix, composed mainly of collagen, is considered responsible for the residual toughness of meat. Matrix metalloproteinases (MMP) responsible for the degradation of connective tissue are found in most tissues, but their participation in meat aging has not been tested. We recently showed that skeletal muscle has multiple MMP activities, as well as regulators and tissue inhibitors of metalloproteinases. Here we present the first observations of physiologic and postmortem variation of MMP activities in muscle. Growing lambs were offered two levels of intake: hay + concentrate for lambs with high growth rate (average daily gain > 250 g) and hay only for those with low growth rate (average daily gain < 25 g). At slaughter and at 21 d of postmortem aging of longissimus, and semimembranosus muscles, we studied collagen content, collagen solubility, free hydroxyproline (OH-pro), and levels of latent and active forms, of a matrix metalloproteinase (MMP-2) by gelatin zymography. Our results demonstrate the presence of an active isoform of MMP-2 in lamb muscle. Its level was higher (+90%, P < 0.01) in lambs that expressed a high growth rate. Activity of MMP-2 was also present at 21 d postmortem, at levels similar to those detected at slaughter. At slaughter and at 21 d, all muscles contained latent MMP-2 and the quantity of proenzyme was greater than that present,in the activated form. The levels of free OH-pro in muscles of lambs with high growth rate increased significantly (P < 0.001) over 21 d from 3.75 to 5.08% of total collagen, and this Was significantly related to the level of active MMP-2 at slaughter. By contrast, the amount of free OH-pro in muscles of lambs with low growth rate was not different at 21 d (1.63% of total OH-pro) than it had been at slaughter (1.84% of total OH-pro). These results suggest that collagen degradation all the way to free amino acids occurs postmortem in muscle and that there are active MMP simultaneously present that may account for this catabolism. The growth rate of animals at slaughter influences collagen turnover in vivo, as well as postmortem collagen degradation.

    Original languageEnglish
    Pages (from-to)1871-1878
    Number of pages8
    JournalJournal of Animal Science
    Volume80
    Issue number7
    Publication statusPublished - Jul 2002

    Keywords

    • aging
    • collagen
    • growth
    • hydroxyproline
    • lambs
    • metalloproteinase
    • INTRAMUSCULAR CONNECTIVE-TISSUE
    • NEWLY SYNTHESIZED COLLAGEN
    • FREE AMINO-ACIDS
    • SEMITENDINOSUS MUSCLE
    • MEAT COLLAGEN
    • HUMAN-SKIN
    • BEEF
    • PALATABILITY
    • TENDERNESS
    • CATHEPSIN

    Cite this

    Elevated rate of collagen solubilization and postmortem degradation in muscles of lambs with high growth rates: Possible relationship with activity of matrix metalloproteinases. / Sylvestre, M N ; Balcerzak, Denis Pierre; Feidt, C ; Baracos, V E ; Bellut, J B .

    In: Journal of Animal Science, Vol. 80, No. 7, 07.2002, p. 1871-1878.

    Research output: Contribution to journalArticle

    @article{4afe4a52d0f54996a0c9c99a84228a9e,
    title = "Elevated rate of collagen solubilization and postmortem degradation in muscles of lambs with high growth rates: Possible relationship with activity of matrix metalloproteinases",
    abstract = "The extracellular matrix, composed mainly of collagen, is considered responsible for the residual toughness of meat. Matrix metalloproteinases (MMP) responsible for the degradation of connective tissue are found in most tissues, but their participation in meat aging has not been tested. We recently showed that skeletal muscle has multiple MMP activities, as well as regulators and tissue inhibitors of metalloproteinases. Here we present the first observations of physiologic and postmortem variation of MMP activities in muscle. Growing lambs were offered two levels of intake: hay + concentrate for lambs with high growth rate (average daily gain > 250 g) and hay only for those with low growth rate (average daily gain < 25 g). At slaughter and at 21 d of postmortem aging of longissimus, and semimembranosus muscles, we studied collagen content, collagen solubility, free hydroxyproline (OH-pro), and levels of latent and active forms, of a matrix metalloproteinase (MMP-2) by gelatin zymography. Our results demonstrate the presence of an active isoform of MMP-2 in lamb muscle. Its level was higher (+90{\%}, P < 0.01) in lambs that expressed a high growth rate. Activity of MMP-2 was also present at 21 d postmortem, at levels similar to those detected at slaughter. At slaughter and at 21 d, all muscles contained latent MMP-2 and the quantity of proenzyme was greater than that present,in the activated form. The levels of free OH-pro in muscles of lambs with high growth rate increased significantly (P < 0.001) over 21 d from 3.75 to 5.08{\%} of total collagen, and this Was significantly related to the level of active MMP-2 at slaughter. By contrast, the amount of free OH-pro in muscles of lambs with low growth rate was not different at 21 d (1.63{\%} of total OH-pro) than it had been at slaughter (1.84{\%} of total OH-pro). These results suggest that collagen degradation all the way to free amino acids occurs postmortem in muscle and that there are active MMP simultaneously present that may account for this catabolism. The growth rate of animals at slaughter influences collagen turnover in vivo, as well as postmortem collagen degradation.",
    keywords = "aging, collagen, growth, hydroxyproline, lambs, metalloproteinase, INTRAMUSCULAR CONNECTIVE-TISSUE, NEWLY SYNTHESIZED COLLAGEN, FREE AMINO-ACIDS, SEMITENDINOSUS MUSCLE, MEAT COLLAGEN, HUMAN-SKIN, BEEF, PALATABILITY, TENDERNESS, CATHEPSIN",
    author = "Sylvestre, {M N} and Balcerzak, {Denis Pierre} and C Feidt and Baracos, {V E} and Bellut, {J B}",
    year = "2002",
    month = "7",
    language = "English",
    volume = "80",
    pages = "1871--1878",
    journal = "Journal of Animal Science",
    issn = "0021-8812",
    publisher = "AMER SOC ANIMAL SCIENCE",
    number = "7",

    }

    TY - JOUR

    T1 - Elevated rate of collagen solubilization and postmortem degradation in muscles of lambs with high growth rates: Possible relationship with activity of matrix metalloproteinases

    AU - Sylvestre, M N

    AU - Balcerzak, Denis Pierre

    AU - Feidt, C

    AU - Baracos, V E

    AU - Bellut, J B

    PY - 2002/7

    Y1 - 2002/7

    N2 - The extracellular matrix, composed mainly of collagen, is considered responsible for the residual toughness of meat. Matrix metalloproteinases (MMP) responsible for the degradation of connective tissue are found in most tissues, but their participation in meat aging has not been tested. We recently showed that skeletal muscle has multiple MMP activities, as well as regulators and tissue inhibitors of metalloproteinases. Here we present the first observations of physiologic and postmortem variation of MMP activities in muscle. Growing lambs were offered two levels of intake: hay + concentrate for lambs with high growth rate (average daily gain > 250 g) and hay only for those with low growth rate (average daily gain < 25 g). At slaughter and at 21 d of postmortem aging of longissimus, and semimembranosus muscles, we studied collagen content, collagen solubility, free hydroxyproline (OH-pro), and levels of latent and active forms, of a matrix metalloproteinase (MMP-2) by gelatin zymography. Our results demonstrate the presence of an active isoform of MMP-2 in lamb muscle. Its level was higher (+90%, P < 0.01) in lambs that expressed a high growth rate. Activity of MMP-2 was also present at 21 d postmortem, at levels similar to those detected at slaughter. At slaughter and at 21 d, all muscles contained latent MMP-2 and the quantity of proenzyme was greater than that present,in the activated form. The levels of free OH-pro in muscles of lambs with high growth rate increased significantly (P < 0.001) over 21 d from 3.75 to 5.08% of total collagen, and this Was significantly related to the level of active MMP-2 at slaughter. By contrast, the amount of free OH-pro in muscles of lambs with low growth rate was not different at 21 d (1.63% of total OH-pro) than it had been at slaughter (1.84% of total OH-pro). These results suggest that collagen degradation all the way to free amino acids occurs postmortem in muscle and that there are active MMP simultaneously present that may account for this catabolism. The growth rate of animals at slaughter influences collagen turnover in vivo, as well as postmortem collagen degradation.

    AB - The extracellular matrix, composed mainly of collagen, is considered responsible for the residual toughness of meat. Matrix metalloproteinases (MMP) responsible for the degradation of connective tissue are found in most tissues, but their participation in meat aging has not been tested. We recently showed that skeletal muscle has multiple MMP activities, as well as regulators and tissue inhibitors of metalloproteinases. Here we present the first observations of physiologic and postmortem variation of MMP activities in muscle. Growing lambs were offered two levels of intake: hay + concentrate for lambs with high growth rate (average daily gain > 250 g) and hay only for those with low growth rate (average daily gain < 25 g). At slaughter and at 21 d of postmortem aging of longissimus, and semimembranosus muscles, we studied collagen content, collagen solubility, free hydroxyproline (OH-pro), and levels of latent and active forms, of a matrix metalloproteinase (MMP-2) by gelatin zymography. Our results demonstrate the presence of an active isoform of MMP-2 in lamb muscle. Its level was higher (+90%, P < 0.01) in lambs that expressed a high growth rate. Activity of MMP-2 was also present at 21 d postmortem, at levels similar to those detected at slaughter. At slaughter and at 21 d, all muscles contained latent MMP-2 and the quantity of proenzyme was greater than that present,in the activated form. The levels of free OH-pro in muscles of lambs with high growth rate increased significantly (P < 0.001) over 21 d from 3.75 to 5.08% of total collagen, and this Was significantly related to the level of active MMP-2 at slaughter. By contrast, the amount of free OH-pro in muscles of lambs with low growth rate was not different at 21 d (1.63% of total OH-pro) than it had been at slaughter (1.84% of total OH-pro). These results suggest that collagen degradation all the way to free amino acids occurs postmortem in muscle and that there are active MMP simultaneously present that may account for this catabolism. The growth rate of animals at slaughter influences collagen turnover in vivo, as well as postmortem collagen degradation.

    KW - aging

    KW - collagen

    KW - growth

    KW - hydroxyproline

    KW - lambs

    KW - metalloproteinase

    KW - INTRAMUSCULAR CONNECTIVE-TISSUE

    KW - NEWLY SYNTHESIZED COLLAGEN

    KW - FREE AMINO-ACIDS

    KW - SEMITENDINOSUS MUSCLE

    KW - MEAT COLLAGEN

    KW - HUMAN-SKIN

    KW - BEEF

    KW - PALATABILITY

    KW - TENDERNESS

    KW - CATHEPSIN

    M3 - Article

    VL - 80

    SP - 1871

    EP - 1878

    JO - Journal of Animal Science

    JF - Journal of Animal Science

    SN - 0021-8812

    IS - 7

    ER -