Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus

Frithjof C Küpper (Corresponding Author), Eric P. Miller, Stephen J. Andrews, Claire Hughes, Lucy J. Carpenter, Wolfram Meyer-Klaucke, Chiaki Toyama, Yasuyuki Muramatsu, Martin C. Feiters, Carl J. Carrano

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Abstract

This study explores key features of bromine and iodine metabolism in the filamentous brown alga and genomics model Ectocarpus siliculosus. Both elements are accumulated in Ectocarpus, albeit at much lower concentration factors (2-3 orders of magnitude for iodine, and < 1 order of magnitude for bromine) than e.g. in the kelp Laminaria digitata. Iodide competitively reduces the accumulation of bromide. Both iodide and bromide are accumulated in the cell wall (apoplast) of Ectocarpus, with minor amounts of bromine also detectable in the cytosol. Ectocarpus emits a range of volatile halogenated compounds, the most prominent of which by far is methyl iodide. Interestingly, biosynthesis of this compound cannot be accounted for by vanadium haloperoxidase since the latter have not been found to catalyze direct halogenation of an unactivated methyl group or hydrocarbon so a methyl halide transferase-type production mechanism is proposed.
Original languageEnglish
Pages (from-to)1119-1128
Number of pages10
JournalJournal of Biological Inorganic Chemistry
Volume23
Issue number7
Early online date9 Mar 2018
DOIs
Publication statusPublished - Oct 2018

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Bromine
Iodine
Genes
Iodides
Genome
Bromides
Kelp
Laminaria
Halogenation
Phaeophyta
Vanadium
Biosynthesis
Algae
Transferases
Hydrocarbons
Genomics
Metabolism
Cytosol
Cell Wall
Cells

Keywords

  • energy-dispersive X-ray analysis
  • halocarbons
  • methyl iodide
  • phaeophyta
  • x-ray absorption spectroscopy

Cite this

Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus. / Küpper, Frithjof C (Corresponding Author); Miller, Eric P.; Andrews, Stephen J. ; Hughes, Claire; Carpenter, Lucy J.; Meyer-Klaucke, Wolfram; Toyama, Chiaki; Muramatsu, Yasuyuki; Feiters, Martin C.; Carrano, Carl J.

In: Journal of Biological Inorganic Chemistry, Vol. 23, No. 7, 10.2018, p. 1119-1128.

Research output: Contribution to journalArticle

Küpper, FC, Miller, EP, Andrews, SJ, Hughes, C, Carpenter, LJ, Meyer-Klaucke, W, Toyama, C, Muramatsu, Y, Feiters, MC & Carrano, CJ 2018, 'Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus', Journal of Biological Inorganic Chemistry, vol. 23, no. 7, pp. 1119-1128. https://doi.org/10.1007/s00775-018-1539-7
Küpper, Frithjof C ; Miller, Eric P. ; Andrews, Stephen J. ; Hughes, Claire ; Carpenter, Lucy J. ; Meyer-Klaucke, Wolfram ; Toyama, Chiaki ; Muramatsu, Yasuyuki ; Feiters, Martin C. ; Carrano, Carl J. / Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus. In: Journal of Biological Inorganic Chemistry. 2018 ; Vol. 23, No. 7. pp. 1119-1128.
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