Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus

Frithjof C Küpper; Eric P. Miller; Stephen J.  Andrews; Claire Hughes; Lucy J. Carpenter; Wolfram Meyer-Klaucke; Chiaki Toyama; Yasuyuki Muramatsu; Martin C. Feiters; Carl J. Carrano

doi:10.1007/s00775-018-1539-7

Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus

Frithjof C Küpper^* (Corresponding Author), Eric P. Miller, Stephen J. Andrews, Claire Hughes, Lucy J. Carpenter, Wolfram Meyer-Klaucke, Chiaki Toyama, Yasuyuki Muramatsu, Martin C. Feiters, Carl J. Carrano

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

10 Downloads (Pure)

Abstract

This study explores key features of bromine and iodine metabolism in the filamentous brown alga and genomics model Ectocarpus siliculosus. Both elements are accumulated in Ectocarpus, albeit at much lower concentration factors (2-3 orders of magnitude for iodine, and < 1 order of magnitude for bromine) than e.g. in the kelp Laminaria digitata. Iodide competitively reduces the accumulation of bromide. Both iodide and bromide are accumulated in the cell wall (apoplast) of Ectocarpus, with minor amounts of bromine also detectable in the cytosol. Ectocarpus emits a range of volatile halogenated compounds, the most prominent of which by far is methyl iodide. Interestingly, biosynthesis of this compound cannot be accounted for by vanadium haloperoxidase since the latter have not been found to catalyze direct halogenation of an unactivated methyl group or hydrocarbon so a methyl halide transferase-type production mechanism is proposed.

Original language	English
Pages (from-to)	1119-1128
Number of pages	10
Journal	Journal of Biological Inorganic Chemistry
Volume	23
Issue number	7
Early online date	9 Mar 2018
DOIs	https://doi.org/10.1007/s00775-018-1539-7
Publication status	Published - Oct 2018

Bibliographical note

Natural Environment Research Council (NERC) Grant numberNE/D521522/1, NE/F012705/1, NE/K000454/1, Oceans 2025 (WP4.5)
National Science Foundation, USA Grant number CHE-1664657
Marine Alliance for Science and Technology for Scotland Grant number HR09011
Open Access via Springer Compact

Keywords

energy-dispersive X-ray analysis
halocarbons
methyl iodide
phaeophyta
x-ray absorption spectroscopy

Access to Document

10.1007/s00775-018-1539-7Licence: CC BY

Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus
© The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Final published version, 2.61 MBLicence: CC BY

Cite this

Küpper, F. C., Miller, E. P., Andrews, S. J., Hughes, C., Carpenter, L. J., Meyer-Klaucke, W., Toyama, C., Muramatsu, Y., Feiters, M. C., & Carrano, C. J. (2018). Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus. Journal of Biological Inorganic Chemistry, 23(7), 1119-1128. https://doi.org/10.1007/s00775-018-1539-7

Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus. / Küpper, Frithjof C (Corresponding Author); Miller, Eric P.; Andrews, Stephen J. et al.
In: Journal of Biological Inorganic Chemistry, Vol. 23, No. 7, 10.2018, p. 1119-1128.

Research output: Contribution to journal › Article › peer-review

Küpper, FC, Miller, EP, Andrews, SJ, Hughes, C, Carpenter, LJ, Meyer-Klaucke, W, Toyama, C, Muramatsu, Y, Feiters, MC & Carrano, CJ 2018, 'Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus', Journal of Biological Inorganic Chemistry, vol. 23, no. 7, pp. 1119-1128. https://doi.org/10.1007/s00775-018-1539-7

@article{94eaac54ea444586af7cceef13b34795,

title = "Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus",

abstract = "This study explores key features of bromine and iodine metabolism in the filamentous brown alga and genomics model Ectocarpus siliculosus. Both elements are accumulated in Ectocarpus, albeit at much lower concentration factors (2-3 orders of magnitude for iodine, and < 1 order of magnitude for bromine) than e.g. in the kelp Laminaria digitata. Iodide competitively reduces the accumulation of bromide. Both iodide and bromide are accumulated in the cell wall (apoplast) of Ectocarpus, with minor amounts of bromine also detectable in the cytosol. Ectocarpus emits a range of volatile halogenated compounds, the most prominent of which by far is methyl iodide. Interestingly, biosynthesis of this compound cannot be accounted for by vanadium haloperoxidase since the latter have not been found to catalyze direct halogenation of an unactivated methyl group or hydrocarbon so a methyl halide transferase-type production mechanism is proposed.",

keywords = "energy-dispersive X-ray analysis, halocarbons, methyl iodide, phaeophyta, x-ray absorption spectroscopy",

author = "K{\"u}pper, {Frithjof C} and Miller, {Eric P.} and Andrews, {Stephen J.} and Claire Hughes and Carpenter, {Lucy J.} and Wolfram Meyer-Klaucke and Chiaki Toyama and Yasuyuki Muramatsu and Feiters, {Martin C.} and Carrano, {Carl J.}",

note = "Natural Environment Research Council (NERC) Grant numberNE/D521522/1, NE/F012705/1, NE/K000454/1, Oceans 2025 (WP4.5) National Science Foundation, USA Grant number CHE-1664657 Marine Alliance for Science and Technology for Scotland Grant number HR09011 Open Access via Springer Compact ",

year = "2018",

month = oct,

doi = "10.1007/s00775-018-1539-7",

language = "English",

volume = "23",

pages = "1119--1128",

journal = "Journal of Biological Inorganic Chemistry",

issn = "0949-8257",

publisher = "Springer ",

number = "7",

}

TY - JOUR

T1 - Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus

AU - Küpper, Frithjof C

AU - Miller, Eric P.

AU - Andrews, Stephen J.

AU - Hughes, Claire

AU - Carpenter, Lucy J.

AU - Meyer-Klaucke, Wolfram

AU - Toyama, Chiaki

AU - Muramatsu, Yasuyuki

AU - Feiters, Martin C.

AU - Carrano, Carl J.

N1 - Natural Environment Research Council (NERC) Grant numberNE/D521522/1, NE/F012705/1, NE/K000454/1, Oceans 2025 (WP4.5) National Science Foundation, USA Grant number CHE-1664657 Marine Alliance for Science and Technology for Scotland Grant number HR09011 Open Access via Springer Compact

PY - 2018/10

Y1 - 2018/10

N2 - This study explores key features of bromine and iodine metabolism in the filamentous brown alga and genomics model Ectocarpus siliculosus. Both elements are accumulated in Ectocarpus, albeit at much lower concentration factors (2-3 orders of magnitude for iodine, and < 1 order of magnitude for bromine) than e.g. in the kelp Laminaria digitata. Iodide competitively reduces the accumulation of bromide. Both iodide and bromide are accumulated in the cell wall (apoplast) of Ectocarpus, with minor amounts of bromine also detectable in the cytosol. Ectocarpus emits a range of volatile halogenated compounds, the most prominent of which by far is methyl iodide. Interestingly, biosynthesis of this compound cannot be accounted for by vanadium haloperoxidase since the latter have not been found to catalyze direct halogenation of an unactivated methyl group or hydrocarbon so a methyl halide transferase-type production mechanism is proposed.

AB - This study explores key features of bromine and iodine metabolism in the filamentous brown alga and genomics model Ectocarpus siliculosus. Both elements are accumulated in Ectocarpus, albeit at much lower concentration factors (2-3 orders of magnitude for iodine, and < 1 order of magnitude for bromine) than e.g. in the kelp Laminaria digitata. Iodide competitively reduces the accumulation of bromide. Both iodide and bromide are accumulated in the cell wall (apoplast) of Ectocarpus, with minor amounts of bromine also detectable in the cytosol. Ectocarpus emits a range of volatile halogenated compounds, the most prominent of which by far is methyl iodide. Interestingly, biosynthesis of this compound cannot be accounted for by vanadium haloperoxidase since the latter have not been found to catalyze direct halogenation of an unactivated methyl group or hydrocarbon so a methyl halide transferase-type production mechanism is proposed.

KW - energy-dispersive X-ray analysis

KW - halocarbons

KW - methyl iodide

KW - phaeophyta

KW - x-ray absorption spectroscopy

U2 - 10.1007/s00775-018-1539-7

DO - 10.1007/s00775-018-1539-7

M3 - Article

SN - 0949-8257

VL - 23

SP - 1119

EP - 1128

JO - Journal of Biological Inorganic Chemistry

JF - Journal of Biological Inorganic Chemistry

IS - 7

ER -

Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this