EMMPRIN (basigin/CD147) Expression is not Correlated With MMP Activity During Adult Mouse Mammary Gland Development

Malgorzata Szymanowska, Kay A. K. Hendry, Claire Robinson, Andreas Kolb

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Extracellular matrix metalloproteinase inducer (EMMPRIN/basigin/CD147) is a cell surface protein, which has been associated with the induction of matrix metalloproteinase (MMP) genes during cancer metastasis. EMMPRIN plays a role in a variety of physiological processes as is evident by the diverse deficiencies detectable in EMMPRIN knockout mice. We have analysed the role of EMMPRIN in the induction of MMP genes during mammary gland differentiation and involution. Co-transfection studies showed that EMMPRIN has diverse effects on MMP promoter activity in different mammary and non-mammary cell lines. Expression of EMMPRIN mRNA is enhanced markedly by insulin in a mammary gland cell line but appears to have no direct effect on MMP gene expression in these cells. Microarray analysis and quantitative PCR show that EMMPRIN is expressed throughout mammary gland differentiation in the mouse. Its expression decreases during early pregnancy and briefly after induction of mammary gland involution by litter removal. Immunohistochemical analysis shows that EMMPRIN expression is limited to the stromal compartment during pregnancy, whereas it is strongly expressed in the epithelium during lactation. In summary the data argue against a causal role for EMMPRIN for the induction of MMP gene expression during adult mammary gland development. These data therefore support a physiological rote for EMMPRIN other than MMP induction in mammary gland biology. J. Cell. Biochem. 106: 52-62, 2009. (C) 2008 Wiley-Liss, Inc.

    Original languageEnglish
    Pages (from-to)52-62
    Number of pages11
    JournalJournal of Cellular Biochemistry
    Volume106
    Issue number1
    Early online date11 Nov 2008
    DOIs
    Publication statusPublished - 1 Jan 2009

    Keywords

    • MAMMARY GLAND
    • DEVELOPMENT
    • MATRIX METALLOPROTEINASE
    • MATRIX-METALLOPROTEINASE INDUCER
    • IMMUNOGLOBULIN SUPERFAMILY
    • EPITHELIAL-CELLS
    • DEGRADING PROTEASES
    • CASEIN GENE
    • INVOLUTION
    • CAVEOLIN-1
    • RECEPTOR
    • CANCER
    • CD147
    • mammary gland
    • development
    • matrix metalloproteinase

    Cite this

    EMMPRIN (basigin/CD147) Expression is not Correlated With MMP Activity During Adult Mouse Mammary Gland Development. / Szymanowska, Malgorzata; Hendry, Kay A. K.; Robinson, Claire; Kolb, Andreas.

    In: Journal of Cellular Biochemistry, Vol. 106, No. 1, 01.01.2009, p. 52-62.

    Research output: Contribution to journalArticle

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    abstract = "Extracellular matrix metalloproteinase inducer (EMMPRIN/basigin/CD147) is a cell surface protein, which has been associated with the induction of matrix metalloproteinase (MMP) genes during cancer metastasis. EMMPRIN plays a role in a variety of physiological processes as is evident by the diverse deficiencies detectable in EMMPRIN knockout mice. We have analysed the role of EMMPRIN in the induction of MMP genes during mammary gland differentiation and involution. Co-transfection studies showed that EMMPRIN has diverse effects on MMP promoter activity in different mammary and non-mammary cell lines. Expression of EMMPRIN mRNA is enhanced markedly by insulin in a mammary gland cell line but appears to have no direct effect on MMP gene expression in these cells. Microarray analysis and quantitative PCR show that EMMPRIN is expressed throughout mammary gland differentiation in the mouse. Its expression decreases during early pregnancy and briefly after induction of mammary gland involution by litter removal. Immunohistochemical analysis shows that EMMPRIN expression is limited to the stromal compartment during pregnancy, whereas it is strongly expressed in the epithelium during lactation. In summary the data argue against a causal role for EMMPRIN for the induction of MMP gene expression during adult mammary gland development. These data therefore support a physiological rote for EMMPRIN other than MMP induction in mammary gland biology. J. Cell. Biochem. 106: 52-62, 2009. (C) 2008 Wiley-Liss, Inc.",
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    AU - Szymanowska, Malgorzata

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    AU - Kolb, Andreas

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    AB - Extracellular matrix metalloproteinase inducer (EMMPRIN/basigin/CD147) is a cell surface protein, which has been associated with the induction of matrix metalloproteinase (MMP) genes during cancer metastasis. EMMPRIN plays a role in a variety of physiological processes as is evident by the diverse deficiencies detectable in EMMPRIN knockout mice. We have analysed the role of EMMPRIN in the induction of MMP genes during mammary gland differentiation and involution. Co-transfection studies showed that EMMPRIN has diverse effects on MMP promoter activity in different mammary and non-mammary cell lines. Expression of EMMPRIN mRNA is enhanced markedly by insulin in a mammary gland cell line but appears to have no direct effect on MMP gene expression in these cells. Microarray analysis and quantitative PCR show that EMMPRIN is expressed throughout mammary gland differentiation in the mouse. Its expression decreases during early pregnancy and briefly after induction of mammary gland involution by litter removal. Immunohistochemical analysis shows that EMMPRIN expression is limited to the stromal compartment during pregnancy, whereas it is strongly expressed in the epithelium during lactation. In summary the data argue against a causal role for EMMPRIN for the induction of MMP gene expression during adult mammary gland development. These data therefore support a physiological rote for EMMPRIN other than MMP induction in mammary gland biology. J. Cell. Biochem. 106: 52-62, 2009. (C) 2008 Wiley-Liss, Inc.

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