Endothelin-1 overexpression and endothelial nitric oxide synthase knock-out induce different pathological responses in the heart of male and female mice

Nicolas Vignon-Zellweger, Katharina Relle, J Rahnenführer, Karima Schwab, Berthold Hocher, Franz Theuring

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Aims: The nitric oxide and endothelin systems are key components of a local paracrine hormone network in the heart. We previously reported that diastolic dysfunction observed in mice lacking the endothelial nitric oxide synthase (eNOS−/−) can be prevented by a genetic overexpression of ET-1. Sexual dimorphisms have been reported in both ET-1 and NO systems. Particularly, eNOS−/− mice present sex related phenotypic differences.

Main methods: We used the ET-1 transgenic (ET+/+), eNOS−/−, and crossbred ET+/+eNOS−/− mice, and wild type controls. We measured cardiac function by heart catheterization. Cardiac ventricles were collected for histological and molecular profiling.

Key findings: We report here that (i) the level of ET-1 expression in eNOS −/− mice was elevated in males but not in females. (ii) Left ventricular end-diastolic blood pressure was higher in male eNOS −/− mice than in females. (ii) eNOS −/− males but not females developed cardiomyocyte hypertrophy. (iv) Perivascular fibrosis of intracardiac arteries developed in female ET +/+ and eNOS −/− mice but not in males. Additionally, (v) the cardiac expression of metalloprotease-9 was higher in eNOS−/− males compared to females. Finally, (vi) cardiac proteome analysis revealed that the protein abundance of the oxidative stress related enzyme superoxide dismutase presented with sexual dimorphism in eNOS−/− and ET+/+ mice.

Significance: These results indicate that the cardiac phenotypes of ET-1 transgenic mice and eNOS knockout mice are sex specific. Since both systems are key players in the pathogenesis of cardiovascular diseases, our findings might be important in the context of gender differences in patients with such diseases.
Original languageEnglish
Pages (from-to)219-225
JournalLife Sciences
Volume118
Issue number2
Early online date16 Dec 2013
DOIs
Publication statusPublished - 24 Nov 2014

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