Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater

J. R. -M. Brown, I. P. Thompson, Graeme Iain Paton, A. C. Singer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aims:

To examine plant terpenoids as inducers of TCE (trichloroethylene) biotransformation by an indigenous microbial community originating from a plume of TCE-contaminated groundwater.

Methods and Results:

One-litre microcosms of groundwater were spiked with 100 mu mol 1-1 of TCE and amended weekly for 16 weeks with 20 mu l 1-1 of the following plant monoterpenes: linalool, pulegone, R-(+) carvone, S-(-) carvone, farnesol, cumene. Yeast extract-amended and unamended control treatments were also prepared. The addition of R-carvone and S-carvone, linalool and cumene resulted in the biotransformation of upwards of 88% of the TCE, significantly more than the unamendment control (61%). The aforementioned group of terpenes also significantly (P < 0 center dot 05) allowed more TCE to be degraded than the remaining two terpenes (farnesol and pulegone), and the yeast extract treatment which biotransformed 74-75% of the TCE. The microbial community profile was monitored by denaturing gradient gel electrophoresis and demonstrated much greater similarities between the microbial communities in terpene-amended treatments than in the yeast extract or unamended controls.

Conclusions:

TCE biotransformation can be significantly enhanced through the addition of selected plant terpenoids.

Significance and Impact of the Study:

Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation.

Original languageEnglish
Pages (from-to)769-774
Number of pages6
JournalLetters in Applied Microbiology
Volume49
Issue number6
Early online date18 Sep 2009
DOIs
Publication statusPublished - Dec 2009

Keywords

  • biostimulation
  • carvone
  • cumene
  • groundwater
  • plant terpenes
  • secondary plant metabolites
  • TCE
  • degrading bacteria
  • ribosomal-RNA
  • trichloroethylene
  • biodegradation
  • Bioremediation
  • metabolites
  • community
  • solvents
  • methane

Cite this

Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. / Brown, J. R. -M.; Thompson, I. P.; Paton, Graeme Iain; Singer, A. C.

In: Letters in Applied Microbiology, Vol. 49, No. 6, 12.2009, p. 769-774.

Research output: Contribution to journalArticle

Brown, J. R. -M. ; Thompson, I. P. ; Paton, Graeme Iain ; Singer, A. C. / Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. In: Letters in Applied Microbiology. 2009 ; Vol. 49, No. 6. pp. 769-774.
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abstract = "Aims:To examine plant terpenoids as inducers of TCE (trichloroethylene) biotransformation by an indigenous microbial community originating from a plume of TCE-contaminated groundwater.Methods and Results:One-litre microcosms of groundwater were spiked with 100 mu mol 1-1 of TCE and amended weekly for 16 weeks with 20 mu l 1-1 of the following plant monoterpenes: linalool, pulegone, R-(+) carvone, S-(-) carvone, farnesol, cumene. Yeast extract-amended and unamended control treatments were also prepared. The addition of R-carvone and S-carvone, linalool and cumene resulted in the biotransformation of upwards of 88{\%} of the TCE, significantly more than the unamendment control (61{\%}). The aforementioned group of terpenes also significantly (P < 0 center dot 05) allowed more TCE to be degraded than the remaining two terpenes (farnesol and pulegone), and the yeast extract treatment which biotransformed 74-75{\%} of the TCE. The microbial community profile was monitored by denaturing gradient gel electrophoresis and demonstrated much greater similarities between the microbial communities in terpene-amended treatments than in the yeast extract or unamended controls.Conclusions:TCE biotransformation can be significantly enhanced through the addition of selected plant terpenoids.Significance and Impact of the Study:Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation.",
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T1 - Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater

AU - Brown, J. R. -M.

AU - Thompson, I. P.

AU - Paton, Graeme Iain

AU - Singer, A. C.

PY - 2009/12

Y1 - 2009/12

N2 - Aims:To examine plant terpenoids as inducers of TCE (trichloroethylene) biotransformation by an indigenous microbial community originating from a plume of TCE-contaminated groundwater.Methods and Results:One-litre microcosms of groundwater were spiked with 100 mu mol 1-1 of TCE and amended weekly for 16 weeks with 20 mu l 1-1 of the following plant monoterpenes: linalool, pulegone, R-(+) carvone, S-(-) carvone, farnesol, cumene. Yeast extract-amended and unamended control treatments were also prepared. The addition of R-carvone and S-carvone, linalool and cumene resulted in the biotransformation of upwards of 88% of the TCE, significantly more than the unamendment control (61%). The aforementioned group of terpenes also significantly (P < 0 center dot 05) allowed more TCE to be degraded than the remaining two terpenes (farnesol and pulegone), and the yeast extract treatment which biotransformed 74-75% of the TCE. The microbial community profile was monitored by denaturing gradient gel electrophoresis and demonstrated much greater similarities between the microbial communities in terpene-amended treatments than in the yeast extract or unamended controls.Conclusions:TCE biotransformation can be significantly enhanced through the addition of selected plant terpenoids.Significance and Impact of the Study:Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation.

AB - Aims:To examine plant terpenoids as inducers of TCE (trichloroethylene) biotransformation by an indigenous microbial community originating from a plume of TCE-contaminated groundwater.Methods and Results:One-litre microcosms of groundwater were spiked with 100 mu mol 1-1 of TCE and amended weekly for 16 weeks with 20 mu l 1-1 of the following plant monoterpenes: linalool, pulegone, R-(+) carvone, S-(-) carvone, farnesol, cumene. Yeast extract-amended and unamended control treatments were also prepared. The addition of R-carvone and S-carvone, linalool and cumene resulted in the biotransformation of upwards of 88% of the TCE, significantly more than the unamendment control (61%). The aforementioned group of terpenes also significantly (P < 0 center dot 05) allowed more TCE to be degraded than the remaining two terpenes (farnesol and pulegone), and the yeast extract treatment which biotransformed 74-75% of the TCE. The microbial community profile was monitored by denaturing gradient gel electrophoresis and demonstrated much greater similarities between the microbial communities in terpene-amended treatments than in the yeast extract or unamended controls.Conclusions:TCE biotransformation can be significantly enhanced through the addition of selected plant terpenoids.Significance and Impact of the Study:Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation.

KW - biostimulation

KW - carvone

KW - cumene

KW - groundwater

KW - plant terpenes

KW - secondary plant metabolites

KW - TCE

KW - degrading bacteria

KW - ribosomal-RNA

KW - trichloroethylene

KW - biodegradation

KW - Bioremediation

KW - metabolites

KW - community

KW - solvents

KW - methane

U2 - 10.1111/j.1472-765X.2009.02738.x

DO - 10.1111/j.1472-765X.2009.02738.x

M3 - Article

VL - 49

SP - 769

EP - 774

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 6

ER -