Enhanced butyrate formation by cross-feeding between Faecalibacterium prausnitzii and Bifidobacterium adolescentis

David Rios-Covian, Miguel Gueimonde, Sylvia H Duncan, Harry J Flint, Clara G de Los Reyes-Gavilan

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Cross-feeding is an important metabolic interaction mechanism of bacterial groups inhabiting the human colon and includes features such as the utilization of acetate by butyrate-producing bacteria as may occur between Bifidobacterium and Faecalibacterium genera. In this study we assessed the utilization of different carbon sources (glucose, starch, inulin and fructo-oligosaccharides) by strains of both genera and selected the best suited combinations for evidencing this cross-feeding phenomenon. Co-cultures of B. adolescentis L2-32 with F. prausnitzii S3/L3 with fructo-oligosaccharides as carbon source, as well as with F. prausnitzii A2-165 in starch, were carried out and the production of short chain fatty acids was determined. In both co-cultures acetate levels decreased between 8 to 24 h of incubation and were lower than in the corresponding B. adolescentis monocultures. In contrast, butyrate concentrations were higher in co-cultures as compared to the respective F. prausnitzii monocultures, indicating enhanced formation of butyrate by F. prausnitzii in the presence of the bifidobacteria. Variations in the levels of acetate and butyrate were more pronounced in the co-culture with fructo-oligosaccharides than with starch. Our results provide a clear demonstration of cross-feeding between B. adolescentis and F. prausnitzii.

Original languageEnglish
Article numberfnv176
Number of pages7
JournalFEMS Microbiology Letters
Volume362
Issue number21
Early online date29 Sep 2015
DOIs
Publication statusPublished - Nov 2015

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Butyrates
Coculture Techniques
Oligosaccharides
Starch
Acetates
Bifidobacterium
Carbon
Inulin
Volatile Fatty Acids
varespladib methyl
Colon
Faecalibacterium prausnitzii
Bifidobacterium adolescentis
Bacteria
Glucose

Keywords

  • faecalibacterium prausnitzii
  • Bifidobacterium adolescentis
  • butyrate
  • acetate
  • starch
  • fructooligosaccharides (FOS)
  • probiotics

Cite this

Enhanced butyrate formation by cross-feeding between Faecalibacterium prausnitzii and Bifidobacterium adolescentis. / Rios-Covian, David; Gueimonde, Miguel; Duncan, Sylvia H; Flint, Harry J; de Los Reyes-Gavilan, Clara G.

In: FEMS Microbiology Letters, Vol. 362, No. 21, fnv176, 11.2015.

Research output: Contribution to journalArticle

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