Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system

O. Benzina, F. Frikha, H. Zouari-Mechichi, S. Woodward, L. Belbahri, E. Mnif, T. Mechichi

Research output: Contribution to journalArticle

Abstract

Response surface methodology (RSM) was applied to optimize the decolourization of the diazo dye Sirius rose BB (SR) by crude laccase from the white-rot fungus Trametes sp. strain CLBE55. A Box–Behnken design using RSM with six variables, namely pH, incubation temperature, enzyme (laccase) concentration, 1-hydroxybenzotriazol (HBT) concentration, dye concentration and incubation time was used in this study to determine significant correlations between the effects of these variables on the decolourization of Sirius rose. The optimum concentrations of HBT, dye and laccase were 0.5 mM, 60 mg/L and 0.1 U/mL, respectively, to obtain maximum decolourization of Sirius rose (approx. 99.5% in 150 min at 45 °C, pH 3). A quadratic model was obtained for dye decolourization using this design. Experimental values were in good agreement with values predicted by the model, giving highly significant correlations.
Original languageEnglish
Pages (from-to)149-157
Number of pages9
Journal3 Biotech
Volume2
Issue number2
Early online date16 Dec 2011
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Azo Compounds
azo dyes
Laccase
decolorization
laccase
dyes
dye
Rosa
Coloring Agents
response surface methodology
incubation
Trametes
white-rot fungi
Fungi
fungus
enzyme
Temperature
Enzymes
enzymes
temperature

Keywords

  • laccase
  • dyes
  • box-behnken
  • decolourization
  • response surface

Cite this

Benzina, O., Frikha, F., Zouari-Mechichi, H., Woodward, S., Belbahri, L., Mnif, E., & Mechichi, T. (2012). Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system. 3 Biotech, 2(2), 149-157. https://doi.org/10.1007/s13205-011-0041-5

Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system. / Benzina, O.; Frikha, F.; Zouari-Mechichi, H.; Woodward, S.; Belbahri, L.; Mnif, E.; Mechichi, T.

In: 3 Biotech, Vol. 2, No. 2, 06.2012, p. 149-157.

Research output: Contribution to journalArticle

Benzina, O, Frikha, F, Zouari-Mechichi, H, Woodward, S, Belbahri, L, Mnif, E & Mechichi, T 2012, 'Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system', 3 Biotech, vol. 2, no. 2, pp. 149-157. https://doi.org/10.1007/s13205-011-0041-5
Benzina O, Frikha F, Zouari-Mechichi H, Woodward S, Belbahri L, Mnif E et al. Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system. 3 Biotech. 2012 Jun;2(2):149-157. https://doi.org/10.1007/s13205-011-0041-5
Benzina, O. ; Frikha, F. ; Zouari-Mechichi, H. ; Woodward, S. ; Belbahri, L. ; Mnif, E. ; Mechichi, T. / Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system. In: 3 Biotech. 2012 ; Vol. 2, No. 2. pp. 149-157.
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