Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system

O. Benzina; F. Frikha; H. Zouari-Mechichi; S. Woodward; L. Belbahri; E. Mnif; T. Mechichi

doi:10.1007/s13205-011-0041-5

Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system

O. Benzina, F. Frikha, H. Zouari-Mechichi, S. Woodward, L. Belbahri, E. Mnif, T. Mechichi

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

Abstract

Response surface methodology (RSM) was applied to optimize the decolourization of the diazo dye Sirius rose BB (SR) by crude laccase from the white-rot fungus Trametes sp. strain CLBE55. A Box–Behnken design using RSM with six variables, namely pH, incubation temperature, enzyme (laccase) concentration, 1-hydroxybenzotriazol (HBT) concentration, dye concentration and incubation time was used in this study to determine significant correlations between the effects of these variables on the decolourization of Sirius rose. The optimum concentrations of HBT, dye and laccase were 0.5 mM, 60 mg/L and 0.1 U/mL, respectively, to obtain maximum decolourization of Sirius rose (approx. 99.5% in 150 min at 45 °C, pH 3). A quadratic model was obtained for dye decolourization using this design. Experimental values were in good agreement with values predicted by the model, giving highly significant correlations.

Original language	English
Pages (from-to)	149-157
Number of pages	9
Journal	3 Biotech
Volume	2
Issue number	2
Early online date	16 Dec 2011
DOIs	https://doi.org/10.1007/s13205-011-0041-5
Publication status	Published - Jun 2012

Keywords

laccase
dyes
box-behnken
decolourization
response surface

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10.1007/s13205-011-0041-5

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title = "Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system",

abstract = "Response surface methodology (RSM) was applied to optimize the decolourization of the diazo dye Sirius rose BB (SR) by crude laccase from the white-rot fungus Trametes sp. strain CLBE55. A Box–Behnken design using RSM with six variables, namely pH, incubation temperature, enzyme (laccase) concentration, 1-hydroxybenzotriazol (HBT) concentration, dye concentration and incubation time was used in this study to determine significant correlations between the effects of these variables on the decolourization of Sirius rose. The optimum concentrations of HBT, dye and laccase were 0.5 mM, 60 mg/L and 0.1 U/mL, respectively, to obtain maximum decolourization of Sirius rose (approx. 99.5% in 150 min at 45 °C, pH 3). A quadratic model was obtained for dye decolourization using this design. Experimental values were in good agreement with values predicted by the model, giving highly significant correlations.",

keywords = "laccase, dyes, box-behnken, decolourization, response surface",

author = "O. Benzina and F. Frikha and H. Zouari-Mechichi and S. Woodward and L. Belbahri and E. Mnif and T. Mechichi",

year = "2012",

month = jun,

doi = "10.1007/s13205-011-0041-5",

language = "English",

volume = "2",

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journal = "3 Biotech",

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T1 - Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system

AU - Benzina, O.

AU - Frikha, F.

AU - Zouari-Mechichi, H.

AU - Woodward, S.

AU - Belbahri, L.

AU - Mnif, E.

AU - Mechichi, T.

PY - 2012/6

Y1 - 2012/6

N2 - Response surface methodology (RSM) was applied to optimize the decolourization of the diazo dye Sirius rose BB (SR) by crude laccase from the white-rot fungus Trametes sp. strain CLBE55. A Box–Behnken design using RSM with six variables, namely pH, incubation temperature, enzyme (laccase) concentration, 1-hydroxybenzotriazol (HBT) concentration, dye concentration and incubation time was used in this study to determine significant correlations between the effects of these variables on the decolourization of Sirius rose. The optimum concentrations of HBT, dye and laccase were 0.5 mM, 60 mg/L and 0.1 U/mL, respectively, to obtain maximum decolourization of Sirius rose (approx. 99.5% in 150 min at 45 °C, pH 3). A quadratic model was obtained for dye decolourization using this design. Experimental values were in good agreement with values predicted by the model, giving highly significant correlations.

AB - Response surface methodology (RSM) was applied to optimize the decolourization of the diazo dye Sirius rose BB (SR) by crude laccase from the white-rot fungus Trametes sp. strain CLBE55. A Box–Behnken design using RSM with six variables, namely pH, incubation temperature, enzyme (laccase) concentration, 1-hydroxybenzotriazol (HBT) concentration, dye concentration and incubation time was used in this study to determine significant correlations between the effects of these variables on the decolourization of Sirius rose. The optimum concentrations of HBT, dye and laccase were 0.5 mM, 60 mg/L and 0.1 U/mL, respectively, to obtain maximum decolourization of Sirius rose (approx. 99.5% in 150 min at 45 °C, pH 3). A quadratic model was obtained for dye decolourization using this design. Experimental values were in good agreement with values predicted by the model, giving highly significant correlations.

KW - laccase

KW - dyes

KW - box-behnken

KW - decolourization

KW - response surface

U2 - 10.1007/s13205-011-0041-5

DO - 10.1007/s13205-011-0041-5

M3 - Article

SN - 2190-572X

VL - 2

SP - 149

EP - 157

JO - 3 Biotech

JF - 3 Biotech

IS - 2

ER -

Enhanced decolourization of the azo dye Sirius rose BB by laccase-HBT system

Abstract

Keywords

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