Enhanced survival of Pseudomonas fluorescens in soil following establishment of inoculum in a sterile soil carrier

M I Van Dyke, J I Prosser

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The method by which bacterial inocula are added to soil can greatly affect survival. This study assessed the establishment of bacteria in a carrier sail, prior to inoculation, as a method of enhancing survival in an agricultural soil. Preincubation of Pseudomons fluorescens in a sterile soil carrier led to greater survival in soil microcosms than preincubation in a non-sterile soil carrier or inoculation of a liquid cell suspension. Increased preincubation time in the sterile soil carrier resulted in greater survival after inoculation. P. fluorescens colonised in a sterile soil carrier for 2 weeks declined in concentration after inoculation by only 2 and 4 log units after 1 and 2 months, respectively, whereas bacteria inoculated using a cell suspension were undetectable by 1 month. Activity of P. fluorescens in soil microcosms, as determined by luminescence, decreased rapidly in all inoculation treatments to undetectable levels by day 4. Results show that establishment and growth using nutrients in sterile soil may adapt cells to soil environmental conditions, thereby increasing survival of bacterial inocula. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1377-1382
Number of pages6
JournalSoil Biology and Biochemistry
Volume32
Publication statusPublished - 2000

Keywords

  • bioluminescence
  • inoculum
  • Pseudomonas fluorescens
  • survival
  • soil
  • KAPPA-CARRAGEENAN
  • AERUGINOSA UG2LR
  • CELLS
  • RHIZOSPHERE
  • BACTERIA
  • STRAIN
  • GROWTH

Cite this

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title = "Enhanced survival of Pseudomonas fluorescens in soil following establishment of inoculum in a sterile soil carrier",
abstract = "The method by which bacterial inocula are added to soil can greatly affect survival. This study assessed the establishment of bacteria in a carrier sail, prior to inoculation, as a method of enhancing survival in an agricultural soil. Preincubation of Pseudomons fluorescens in a sterile soil carrier led to greater survival in soil microcosms than preincubation in a non-sterile soil carrier or inoculation of a liquid cell suspension. Increased preincubation time in the sterile soil carrier resulted in greater survival after inoculation. P. fluorescens colonised in a sterile soil carrier for 2 weeks declined in concentration after inoculation by only 2 and 4 log units after 1 and 2 months, respectively, whereas bacteria inoculated using a cell suspension were undetectable by 1 month. Activity of P. fluorescens in soil microcosms, as determined by luminescence, decreased rapidly in all inoculation treatments to undetectable levels by day 4. Results show that establishment and growth using nutrients in sterile soil may adapt cells to soil environmental conditions, thereby increasing survival of bacterial inocula. (C) 2000 Elsevier Science Ltd. All rights reserved.",
keywords = "bioluminescence, inoculum, Pseudomonas fluorescens, survival, soil, KAPPA-CARRAGEENAN, AERUGINOSA UG2LR, CELLS, RHIZOSPHERE, BACTERIA, STRAIN, GROWTH",
author = "{Van Dyke}, {M I} and Prosser, {J I}",
year = "2000",
language = "English",
volume = "32",
pages = "1377--1382",
journal = "Soil Biology and Biochemistry",
issn = "0038-0717",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Enhanced survival of Pseudomonas fluorescens in soil following establishment of inoculum in a sterile soil carrier

AU - Van Dyke, M I

AU - Prosser, J I

PY - 2000

Y1 - 2000

N2 - The method by which bacterial inocula are added to soil can greatly affect survival. This study assessed the establishment of bacteria in a carrier sail, prior to inoculation, as a method of enhancing survival in an agricultural soil. Preincubation of Pseudomons fluorescens in a sterile soil carrier led to greater survival in soil microcosms than preincubation in a non-sterile soil carrier or inoculation of a liquid cell suspension. Increased preincubation time in the sterile soil carrier resulted in greater survival after inoculation. P. fluorescens colonised in a sterile soil carrier for 2 weeks declined in concentration after inoculation by only 2 and 4 log units after 1 and 2 months, respectively, whereas bacteria inoculated using a cell suspension were undetectable by 1 month. Activity of P. fluorescens in soil microcosms, as determined by luminescence, decreased rapidly in all inoculation treatments to undetectable levels by day 4. Results show that establishment and growth using nutrients in sterile soil may adapt cells to soil environmental conditions, thereby increasing survival of bacterial inocula. (C) 2000 Elsevier Science Ltd. All rights reserved.

AB - The method by which bacterial inocula are added to soil can greatly affect survival. This study assessed the establishment of bacteria in a carrier sail, prior to inoculation, as a method of enhancing survival in an agricultural soil. Preincubation of Pseudomons fluorescens in a sterile soil carrier led to greater survival in soil microcosms than preincubation in a non-sterile soil carrier or inoculation of a liquid cell suspension. Increased preincubation time in the sterile soil carrier resulted in greater survival after inoculation. P. fluorescens colonised in a sterile soil carrier for 2 weeks declined in concentration after inoculation by only 2 and 4 log units after 1 and 2 months, respectively, whereas bacteria inoculated using a cell suspension were undetectable by 1 month. Activity of P. fluorescens in soil microcosms, as determined by luminescence, decreased rapidly in all inoculation treatments to undetectable levels by day 4. Results show that establishment and growth using nutrients in sterile soil may adapt cells to soil environmental conditions, thereby increasing survival of bacterial inocula. (C) 2000 Elsevier Science Ltd. All rights reserved.

KW - bioluminescence

KW - inoculum

KW - Pseudomonas fluorescens

KW - survival

KW - soil

KW - KAPPA-CARRAGEENAN

KW - AERUGINOSA UG2LR

KW - CELLS

KW - RHIZOSPHERE

KW - BACTERIA

KW - STRAIN

KW - GROWTH

M3 - Article

VL - 32

SP - 1377

EP - 1382

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

ER -