Abstract
MicroRNAs (miRNAs) are small noncoding molecules that control gene expression posttranscriptionally, with microRNA-155 (miR-155) one of the first to be implicated in immune regulation. Here, we show that miR-155-deficient mice are less able to eradicate a mucosal Citrobacter rodentium infection than wild-type C57BL/6 mice. miR-155-deficient mice exhibited prolonged colonization associated with a higher C. rodentium burden in gastrointestinal tissue and spread into systemic tissues. Germinal center formation and humoral immune responses against C. rodentium were severely impaired in infected miR-155-deficient mice. A similarly susceptible phenotype was observed in μMT mice reconstituted with miR-155-deficient B cells, indicating that miR-155 is required intrinsically for mediating protection against this predominantly luminal bacterial pathogen.
Original language | English |
---|---|
Pages (from-to) | 723-732 |
Number of pages | 10 |
Journal | Infection and Immunity |
Volume | 81 |
Issue number | 3 |
Early online date | 21 Dec 2012 |
DOIs | |
Publication status | Published - Mar 2013 |
Keywords
- Animals
- Citrobacter rodentium
- Colitis
- Enterobacteriaceae Infections
- Female
- Gene Expression Regulation
- Genetic Predisposition to Disease
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- MicroRNAs
- RNA, Ribosomal, 16S
- Real-Time Polymerase Chain Reaction
- Transcriptome