Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice

Simon Clare, Victoria John, Alan W Walker, Jennifer L Hill, Cei Abreu-Goodger, Christine Hale, David Goulding, Trevor D Lawley, Pietro Mastroeni, Gadi Frankel, Anton J Enright, Elena Vigorito, Gordon Dougan

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small noncoding molecules that control gene expression posttranscriptionally, with microRNA-155 (miR-155) one of the first to be implicated in immune regulation. Here, we show that miR-155-deficient mice are less able to eradicate a mucosal Citrobacter rodentium infection than wild-type C57BL/6 mice. miR-155-deficient mice exhibited prolonged colonization associated with a higher C. rodentium burden in gastrointestinal tissue and spread into systemic tissues. Germinal center formation and humoral immune responses against C. rodentium were severely impaired in infected miR-155-deficient mice. A similarly susceptible phenotype was observed in μMT mice reconstituted with miR-155-deficient B cells, indicating that miR-155 is required intrinsically for mediating protection against this predominantly luminal bacterial pathogen.
Original languageEnglish
Pages (from-to)723-732
Number of pages10
JournalInfection and Immunity
Volume81
Issue number3
Early online date21 Dec 2012
DOIs
Publication statusPublished - Mar 2013

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Citrobacter rodentium
MicroRNAs
Infection
Germinal Center
Humoral Immunity
Inbred C57BL Mouse
B-Lymphocytes
Phenotype
Gene Expression
Mouse Mirn155 microRNA

Keywords

  • Animals
  • Citrobacter rodentium
  • Colitis
  • Enterobacteriaceae Infections
  • Female
  • Gene Expression Regulation
  • Genetic Predisposition to Disease
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • MicroRNAs
  • RNA, Ribosomal, 16S
  • Real-Time Polymerase Chain Reaction
  • Transcriptome

Cite this

Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice. / Clare, Simon; John, Victoria; Walker, Alan W; Hill, Jennifer L; Abreu-Goodger, Cei; Hale, Christine; Goulding, David; Lawley, Trevor D; Mastroeni, Pietro; Frankel, Gadi; Enright, Anton J; Vigorito, Elena; Dougan, Gordon.

In: Infection and Immunity, Vol. 81, No. 3, 03.2013, p. 723-732.

Research output: Contribution to journalArticle

Clare, S, John, V, Walker, AW, Hill, JL, Abreu-Goodger, C, Hale, C, Goulding, D, Lawley, TD, Mastroeni, P, Frankel, G, Enright, AJ, Vigorito, E & Dougan, G 2013, 'Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice' Infection and Immunity, vol. 81, no. 3, pp. 723-732. https://doi.org/10.1128/IAI.00969-12
Clare, Simon ; John, Victoria ; Walker, Alan W ; Hill, Jennifer L ; Abreu-Goodger, Cei ; Hale, Christine ; Goulding, David ; Lawley, Trevor D ; Mastroeni, Pietro ; Frankel, Gadi ; Enright, Anton J ; Vigorito, Elena ; Dougan, Gordon. / Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice. In: Infection and Immunity. 2013 ; Vol. 81, No. 3. pp. 723-732.
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KW - Animals

KW - Citrobacter rodentium

KW - Colitis

KW - Enterobacteriaceae Infections

KW - Female

KW - Gene Expression Regulation

KW - Genetic Predisposition to Disease

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

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KW - RNA, Ribosomal, 16S

KW - Real-Time Polymerase Chain Reaction

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