Erythrocyte membrane fatty acid composition of smokers and non-smokers

effects of vitamin E supplementation

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: Adequate dietary intakes of vitamin E and essential polyunsaturated fatty acids are important to maintain cell membrane integrity, and deficiencies have been associated with smoking related cardiovascular disease. Sufficient vitamin E is required to prevent free radical mediated peroxidation of membrane lipids. Consequently, smokers may have a greater requirement for this antioxidant. To investigate, we assessed the concurrent influences of smoking, vitamin E supplementation and red blood cell (RBC) PUFA composition on the susceptibility of the cells to lipid peroxidation in adult males.

Design and subjects: Thirty male smokers and thirty male non-smokers were randomly ascribed to daily 280 mg vitamin E or placebo supplements for 10 weeks. RBC were analysed at weeks 0 and IO for fatty acid methyl esters, vitamin E, and their susceptibility to in vitro H2O2 induced lipid peroxidation.

Results: Concentrations of essential fatty acids (EFA) in RBC were lower in smokers than in non-smokers. Supplementation with vitamin E increased levers of RBC EFA in smokers to match those of non-smokers. Furthermore, the ratio of vitamin E to PUFA in RBC from smokers and non-smokers was inversely correlated with their susceptibility to peroxidation.

Conclusions: An adequate vitamin E to PUFA ratio is required to protect cell membranes from oxidative damage. The significant correlation between susceptibility to peroxidation and the PUFA content of RBC before supplementation suggests an inadequate intake of vitamin E in relation to PUFA intake. Moreover. the requirement for vitamin E appears to be greater in smokers than in non-smokers.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalEuropean Journal of Clinical Nutrition
Volume52
Issue number2
Publication statusPublished - Feb 1998

Keywords

  • vitamin E
  • polyunsaturated fatty acids
  • lipid peroxidation
  • smoking
  • lipid-peroxidation
  • cigarette-smoke
  • plasma-lipoproteins
  • respiratory burst
  • hydrogen-peroxide
  • free-radicals
  • blood
  • antioxidant
  • nonsmokers
  • tissue

Cite this

@article{bd5cd21173c444b58d346177e2897640,
title = "Erythrocyte membrane fatty acid composition of smokers and non-smokers: effects of vitamin E supplementation",
abstract = "Objective: Adequate dietary intakes of vitamin E and essential polyunsaturated fatty acids are important to maintain cell membrane integrity, and deficiencies have been associated with smoking related cardiovascular disease. Sufficient vitamin E is required to prevent free radical mediated peroxidation of membrane lipids. Consequently, smokers may have a greater requirement for this antioxidant. To investigate, we assessed the concurrent influences of smoking, vitamin E supplementation and red blood cell (RBC) PUFA composition on the susceptibility of the cells to lipid peroxidation in adult males.Design and subjects: Thirty male smokers and thirty male non-smokers were randomly ascribed to daily 280 mg vitamin E or placebo supplements for 10 weeks. RBC were analysed at weeks 0 and IO for fatty acid methyl esters, vitamin E, and their susceptibility to in vitro H2O2 induced lipid peroxidation.Results: Concentrations of essential fatty acids (EFA) in RBC were lower in smokers than in non-smokers. Supplementation with vitamin E increased levers of RBC EFA in smokers to match those of non-smokers. Furthermore, the ratio of vitamin E to PUFA in RBC from smokers and non-smokers was inversely correlated with their susceptibility to peroxidation.Conclusions: An adequate vitamin E to PUFA ratio is required to protect cell membranes from oxidative damage. The significant correlation between susceptibility to peroxidation and the PUFA content of RBC before supplementation suggests an inadequate intake of vitamin E in relation to PUFA intake. Moreover. the requirement for vitamin E appears to be greater in smokers than in non-smokers.",
keywords = "vitamin E, polyunsaturated fatty acids, lipid peroxidation, smoking, lipid-peroxidation, cigarette-smoke, plasma-lipoproteins, respiratory burst, hydrogen-peroxide, free-radicals, blood, antioxidant, nonsmokers, tissue",
author = "Brown, {K M} and Morrice, {P C} and Duthie, {G G}",
year = "1998",
month = "2",
language = "English",
volume = "52",
pages = "145--150",
journal = "European Journal of Clinical Nutrition",
issn = "0954-3007",
publisher = "NATURE PUBLISHING GROUP",
number = "2",

}

TY - JOUR

T1 - Erythrocyte membrane fatty acid composition of smokers and non-smokers

T2 - effects of vitamin E supplementation

AU - Brown, K M

AU - Morrice, P C

AU - Duthie, G G

PY - 1998/2

Y1 - 1998/2

N2 - Objective: Adequate dietary intakes of vitamin E and essential polyunsaturated fatty acids are important to maintain cell membrane integrity, and deficiencies have been associated with smoking related cardiovascular disease. Sufficient vitamin E is required to prevent free radical mediated peroxidation of membrane lipids. Consequently, smokers may have a greater requirement for this antioxidant. To investigate, we assessed the concurrent influences of smoking, vitamin E supplementation and red blood cell (RBC) PUFA composition on the susceptibility of the cells to lipid peroxidation in adult males.Design and subjects: Thirty male smokers and thirty male non-smokers were randomly ascribed to daily 280 mg vitamin E or placebo supplements for 10 weeks. RBC were analysed at weeks 0 and IO for fatty acid methyl esters, vitamin E, and their susceptibility to in vitro H2O2 induced lipid peroxidation.Results: Concentrations of essential fatty acids (EFA) in RBC were lower in smokers than in non-smokers. Supplementation with vitamin E increased levers of RBC EFA in smokers to match those of non-smokers. Furthermore, the ratio of vitamin E to PUFA in RBC from smokers and non-smokers was inversely correlated with their susceptibility to peroxidation.Conclusions: An adequate vitamin E to PUFA ratio is required to protect cell membranes from oxidative damage. The significant correlation between susceptibility to peroxidation and the PUFA content of RBC before supplementation suggests an inadequate intake of vitamin E in relation to PUFA intake. Moreover. the requirement for vitamin E appears to be greater in smokers than in non-smokers.

AB - Objective: Adequate dietary intakes of vitamin E and essential polyunsaturated fatty acids are important to maintain cell membrane integrity, and deficiencies have been associated with smoking related cardiovascular disease. Sufficient vitamin E is required to prevent free radical mediated peroxidation of membrane lipids. Consequently, smokers may have a greater requirement for this antioxidant. To investigate, we assessed the concurrent influences of smoking, vitamin E supplementation and red blood cell (RBC) PUFA composition on the susceptibility of the cells to lipid peroxidation in adult males.Design and subjects: Thirty male smokers and thirty male non-smokers were randomly ascribed to daily 280 mg vitamin E or placebo supplements for 10 weeks. RBC were analysed at weeks 0 and IO for fatty acid methyl esters, vitamin E, and their susceptibility to in vitro H2O2 induced lipid peroxidation.Results: Concentrations of essential fatty acids (EFA) in RBC were lower in smokers than in non-smokers. Supplementation with vitamin E increased levers of RBC EFA in smokers to match those of non-smokers. Furthermore, the ratio of vitamin E to PUFA in RBC from smokers and non-smokers was inversely correlated with their susceptibility to peroxidation.Conclusions: An adequate vitamin E to PUFA ratio is required to protect cell membranes from oxidative damage. The significant correlation between susceptibility to peroxidation and the PUFA content of RBC before supplementation suggests an inadequate intake of vitamin E in relation to PUFA intake. Moreover. the requirement for vitamin E appears to be greater in smokers than in non-smokers.

KW - vitamin E

KW - polyunsaturated fatty acids

KW - lipid peroxidation

KW - smoking

KW - lipid-peroxidation

KW - cigarette-smoke

KW - plasma-lipoproteins

KW - respiratory burst

KW - hydrogen-peroxide

KW - free-radicals

KW - blood

KW - antioxidant

KW - nonsmokers

KW - tissue

M3 - Article

VL - 52

SP - 145

EP - 150

JO - European Journal of Clinical Nutrition

JF - European Journal of Clinical Nutrition

SN - 0954-3007

IS - 2

ER -