TY - JOUR
T1 - Establishing a macrophage model of inflammasome activation under conditions mimicking sepsis to characterise the effects of melatonin
AU - Musatti, Elena
AU - Galley, Helen F.
AU - Webster, Nigel
N1 - Acknowledgements: We acknowledge the support of the BJA/RCoA for a PhD studentship for Elena Musatti.
PY - 2019/10
Y1 - 2019/10
N2 - Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated inflammatory host response to infection. Melatonin has antioxidant and anti-inflammatory activity and is currently under trial as a treatment for sepsis. The inflammasome is a cytosolic protein complex that intensifies inflammation in response to signals pathogen- and danger-associated molecular patterns, extracellular adenosine 5′-triphosphate (ATP), metabolic dysfunction, K+ efflux, and mitochondrial DNA. Once active, the inflammasome triggers caspase-1-dependent maturation of interleukin (IL)-1β, an important pro-inflammatory cytokine. Nigericin is a polyether ionophore which catalyses exchange of K+. The aim of this study was to develop an inflammasome activation model in macrophages under conditions mimicking sepsis using nigericin as a specific activator, in order to determine the effects of melatonin. In this study we describe the characterisation of the model.
AB - Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated inflammatory host response to infection. Melatonin has antioxidant and anti-inflammatory activity and is currently under trial as a treatment for sepsis. The inflammasome is a cytosolic protein complex that intensifies inflammation in response to signals pathogen- and danger-associated molecular patterns, extracellular adenosine 5′-triphosphate (ATP), metabolic dysfunction, K+ efflux, and mitochondrial DNA. Once active, the inflammasome triggers caspase-1-dependent maturation of interleukin (IL)-1β, an important pro-inflammatory cytokine. Nigericin is a polyether ionophore which catalyses exchange of K+. The aim of this study was to develop an inflammasome activation model in macrophages under conditions mimicking sepsis using nigericin as a specific activator, in order to determine the effects of melatonin. In this study we describe the characterisation of the model.
U2 - 10.1016/j.bja.2019.04.011
DO - 10.1016/j.bja.2019.04.011
M3 - Abstract
SN - 0007-0912
VL - 123
SP - E496
JO - British Journal of Anaesthesia
JF - British Journal of Anaesthesia
IS - 4
T2 - BJA Research Forum
Y2 - 8 May 2019 through 9 May 2019
ER -