Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests

P. Joana Dias, Seema Fotedar, Julieta Munoz, Matthew J. Hewitt, Sherralee Lukehurst, Mathew Hourston, Claire Wellington, Roger Duggan, Samantha Bridgwood, Marion Massam, Victoria Aitken, Paul de Lestang, Simon McKirdy, Richard Willan, Lisa Kirkendale, Jennifer Giannetta, Maria Corsini-Foka, Steve Pothoven, Fiona Gower, Frédérique ViardChristian Buschbaum, Giuseppe Scarcella, Pierluigi Strafella, Melanie J. Bishop, Timothy Sullivan, Isabella Buttino, Hawis Madduppa, Mareike Huhn, Chela J. Zabin, Karolina Bacela-Spychalska, Dagmara Wójcik-Fudalewska, Alexandra Markert, Alexey Maximov, Lena Kautsky, Cornelia Jaspers, Jonne Kotta, Merli Pärnoja, Daniel Robledo, Konstantinos Tsiamis, Frithjof C. Kuepper, Ante Žuljević, Justin I. McDonald, Michael Snow

Research output: Contribution to journalArticle

12 Citations (Scopus)
3 Downloads (Pure)

Abstract

Introduced Marine Pests (IMP, = non-indigenous marine species) prevention, early detection and risk-based management strategies have become the priority for biosecurity operations worldwide, in recognition of the fact that, once established, the effective management of marine pests can rapidly become cost prohibitive or impractical. In Western Australia (WA), biosecurity management is guided by the “Western Australian Prevention List for Introduced Marine Pests” which is a policy tool that details species or genera as being of high risk to the region. This list forms the basis of management efforts to prevent introduction of these species, monitoring efforts to detect them at an early stage, and rapid response should they be detected. It is therefore essential that the species listed can be rapid and confidently identified and discriminated from native species by a range of government and industry stakeholders. Recognising that identification of these species requires very specialist expertise which may be in short supply and not readily accessible in a regulatory environment, and the fact that much publicly available data is not verifiable or suitable for regulatory enforcement, the WA government commissioned the current project to collate a reference collection of these marine pest specimens. In this work, we thus established collaboration with researchers worldwide in order to source representative specimens of the species listed. Our main objective was to build a reference collection of taxonomically vouchered specimens and subsequently to generate species-specific DNA barcodes suited to supporting their future identification. To date, we were able to obtain specimens of 75 species (representative of all but four of the pests listed) which have been identified by experts and placed with the WA Government Department of Fisheries and, where possible, in accessible museums and institutions in Australasia. The reference collection supports the fast and reliable taxonomic and molecular identification of marine pests in WA and constitutes a valuable resource for training of stakeholders with interest in IMP recognition in Australia. The reference collection is also useful in supporting the development of a variety of DNA-based detection strategies such as real-time PCR and metabarcoding of complex environmental samples (e.g. biofouling ommunities). The Prevention List is under regular review to ensure its continued relevance and that it remains evidence and risk-based. Similarly, its associated reference collection also remains to some extent a work in progress. In recognition of this fact, this report seeks to provide details of this continually evolving information repository publicly available to the biosecurity management community worldwide.
Original languageEnglish
Pages (from-to)215-225
Number of pages11
JournalManagement of Biological Invasions
Volume8
Issue number2
Early online date30 Dec 2016
DOIs
Publication statusPublished - Jun 2017
Event9th International Conference on Marine Bioinvasions (ICMB-IX) - Sydney, Australia
Duration: 19 Jan 201621 Jan 2016

Fingerprint

pests
Western Australia
biosecurity
stakeholders
biofouling
stakeholder
Australasian region
DNA barcoding
DNA
species identification
pest
quantitative polymerase chain reaction
indigenous species
researchers
fisheries
repository
native species
museum
industry
monitoring

Keywords

  • Introduced Marine Pests (IMP)
  • taxonomic voucher
  • cytochrome c oxidase I
  • COI
  • The Barcode of Life Data System
  • BoLD
  • marine biosecurity

Cite this

Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests. / Dias, P. Joana; Fotedar, Seema; Munoz, Julieta; Hewitt, Matthew J.; Lukehurst, Sherralee; Hourston, Mathew; Wellington, Claire; Duggan, Roger; Bridgwood, Samantha; Massam, Marion; Aitken, Victoria; de Lestang, Paul; McKirdy, Simon; Willan, Richard; Kirkendale, Lisa; Giannetta, Jennifer; Corsini-Foka, Maria; Pothoven, Steve; Gower, Fiona; Viard, Frédérique; Buschbaum, Christian; Scarcella, Giuseppe; Strafella, Pierluigi; Bishop, Melanie J.; Sullivan, Timothy; Buttino, Isabella; Madduppa, Hawis; Huhn, Mareike; Zabin, Chela J.; Bacela-Spychalska, Karolina; Wójcik-Fudalewska, Dagmara; Markert, Alexandra; Maximov, Alexey; Kautsky, Lena; Jaspers, Cornelia; Kotta, Jonne; Pärnoja, Merli; Robledo, Daniel; Tsiamis, Konstantinos; Kuepper, Frithjof C.; Žuljević, Ante; McDonald, Justin I.; Snow, Michael.

In: Management of Biological Invasions, Vol. 8, No. 2, 06.2017, p. 215-225.

Research output: Contribution to journalArticle

Dias, PJ, Fotedar, S, Munoz, J, Hewitt, MJ, Lukehurst, S, Hourston, M, Wellington, C, Duggan, R, Bridgwood, S, Massam, M, Aitken, V, de Lestang, P, McKirdy, S, Willan, R, Kirkendale, L, Giannetta, J, Corsini-Foka, M, Pothoven, S, Gower, F, Viard, F, Buschbaum, C, Scarcella, G, Strafella, P, Bishop, MJ, Sullivan, T, Buttino, I, Madduppa, H, Huhn, M, Zabin, CJ, Bacela-Spychalska, K, Wójcik-Fudalewska, D, Markert, A, Maximov, A, Kautsky, L, Jaspers, C, Kotta, J, Pärnoja, M, Robledo, D, Tsiamis, K, Kuepper, FC, Žuljević, A, McDonald, JI & Snow, M 2017, 'Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests', Management of Biological Invasions, vol. 8, no. 2, pp. 215-225. https://doi.org/10.3391/mbi.2017.8.2.09
Dias, P. Joana ; Fotedar, Seema ; Munoz, Julieta ; Hewitt, Matthew J. ; Lukehurst, Sherralee ; Hourston, Mathew ; Wellington, Claire ; Duggan, Roger ; Bridgwood, Samantha ; Massam, Marion ; Aitken, Victoria ; de Lestang, Paul ; McKirdy, Simon ; Willan, Richard ; Kirkendale, Lisa ; Giannetta, Jennifer ; Corsini-Foka, Maria ; Pothoven, Steve ; Gower, Fiona ; Viard, Frédérique ; Buschbaum, Christian ; Scarcella, Giuseppe ; Strafella, Pierluigi ; Bishop, Melanie J. ; Sullivan, Timothy ; Buttino, Isabella ; Madduppa, Hawis ; Huhn, Mareike ; Zabin, Chela J. ; Bacela-Spychalska, Karolina ; Wójcik-Fudalewska, Dagmara ; Markert, Alexandra ; Maximov, Alexey ; Kautsky, Lena ; Jaspers, Cornelia ; Kotta, Jonne ; Pärnoja, Merli ; Robledo, Daniel ; Tsiamis, Konstantinos ; Kuepper, Frithjof C. ; Žuljević, Ante ; McDonald, Justin I. ; Snow, Michael. / Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests. In: Management of Biological Invasions. 2017 ; Vol. 8, No. 2. pp. 215-225.
@article{bc236824cb734d57a6b3fa18399a0724,
title = "Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests",
abstract = "Introduced Marine Pests (IMP, = non-indigenous marine species) prevention, early detection and risk-based management strategies have become the priority for biosecurity operations worldwide, in recognition of the fact that, once established, the effective management of marine pests can rapidly become cost prohibitive or impractical. In Western Australia (WA), biosecurity management is guided by the “Western Australian Prevention List for Introduced Marine Pests” which is a policy tool that details species or genera as being of high risk to the region. This list forms the basis of management efforts to prevent introduction of these species, monitoring efforts to detect them at an early stage, and rapid response should they be detected. It is therefore essential that the species listed can be rapid and confidently identified and discriminated from native species by a range of government and industry stakeholders. Recognising that identification of these species requires very specialist expertise which may be in short supply and not readily accessible in a regulatory environment, and the fact that much publicly available data is not verifiable or suitable for regulatory enforcement, the WA government commissioned the current project to collate a reference collection of these marine pest specimens. In this work, we thus established collaboration with researchers worldwide in order to source representative specimens of the species listed. Our main objective was to build a reference collection of taxonomically vouchered specimens and subsequently to generate species-specific DNA barcodes suited to supporting their future identification. To date, we were able to obtain specimens of 75 species (representative of all but four of the pests listed) which have been identified by experts and placed with the WA Government Department of Fisheries and, where possible, in accessible museums and institutions in Australasia. The reference collection supports the fast and reliable taxonomic and molecular identification of marine pests in WA and constitutes a valuable resource for training of stakeholders with interest in IMP recognition in Australia. The reference collection is also useful in supporting the development of a variety of DNA-based detection strategies such as real-time PCR and metabarcoding of complex environmental samples (e.g. biofouling ommunities). The Prevention List is under regular review to ensure its continued relevance and that it remains evidence and risk-based. Similarly, its associated reference collection also remains to some extent a work in progress. In recognition of this fact, this report seeks to provide details of this continually evolving information repository publicly available to the biosecurity management community worldwide.",
keywords = "Introduced Marine Pests (IMP), taxonomic voucher, cytochrome c oxidase I, COI, The Barcode of Life Data System, BoLD, marine biosecurity",
author = "Dias, {P. Joana} and Seema Fotedar and Julieta Munoz and Hewitt, {Matthew J.} and Sherralee Lukehurst and Mathew Hourston and Claire Wellington and Roger Duggan and Samantha Bridgwood and Marion Massam and Victoria Aitken and {de Lestang}, Paul and Simon McKirdy and Richard Willan and Lisa Kirkendale and Jennifer Giannetta and Maria Corsini-Foka and Steve Pothoven and Fiona Gower and Fr{\'e}d{\'e}rique Viard and Christian Buschbaum and Giuseppe Scarcella and Pierluigi Strafella and Bishop, {Melanie J.} and Timothy Sullivan and Isabella Buttino and Hawis Madduppa and Mareike Huhn and Zabin, {Chela J.} and Karolina Bacela-Spychalska and Dagmara W{\'o}jcik-Fudalewska and Alexandra Markert and Alexey Maximov and Lena Kautsky and Cornelia Jaspers and Jonne Kotta and Merli P{\"a}rnoja and Daniel Robledo and Konstantinos Tsiamis and Kuepper, {Frithjof C.} and Ante Žuljević and McDonald, {Justin I.} and Michael Snow",
note = "We acknowledge field sample collection assistance of the WA Government Department of Fisheries (DoF) Biosecurity Research and Compliance teams in obtaining specimens of IMP present in Australia. We also would like to acknowledge field sampling and cooperation of stakeholders in WA. We are most grateful to everyone in Australia and abroad that provided us with specimens including John Lewis at ES Link Services, Craig Boys and Anthony Fowler at the NSW Department of Primary Industries, Simon Grove and Kirrily Moore at the Department of State Growth in Tasmania, and Matt Koopman at Fishwell Consulting in Australia; Eric A. Hoffman at University of Central Florida, Ximing Guo at Rutgers University, Steve Palumbi at Stanford University, Charles Epifanio at the University of Delaware, Toni Renee Ignoffo and Wim Kimmerer at San Francisco State University, Joseph Pawlik and Jack Cushman Koch at the University of North Carolina Wilmington and Kimberly S. Reece at the Virginia Institute of Marine Science, USA; Keith Hiscock at Plymouth Marine Biological Association and Elizabeth Cook at the Scottish Association for Marine Science, UK; Per Jonsson and Gunnar Cervin at the University of Gothenburg and Ann-Britt Florin at the Swedish University of Agricultural Sciences, Sweden; Bente Edvardsen and Wenche Eikrem at the University of Oslo, Norway; Cynthia McKenzie at the Department of Fisheries and Oceans Canada; Annick Verween at Ghent University, Belgium; Nathalie Cochennec-Laureau at IFREMER, France; Bartlomiej Arciszewski at the University of Gdansk, Poland; Marcus Anders Krag and Peter Rask Moller at the Natural History Museum of Denmark; Taeko Kimura at Mie University, Japan; Xiao Liu at the Institute of Oceanology in Qingdao, China; Tsang Ling Ming at National Taiwan Ocean University; Ho Young Soh at Chonnam National University, Republic of Korea; Ronaldo Sousa at University of Minho and Alexandra Teodosio at the University of Algarve, Portugal. We would like to acknowledge the most valuable ongoing support of John Huisman at the Western Australian Herbarium and Serena Wilkens and Mike Page at the New Zealand Marine Invasive Taxonomic Service (MITS) and the National Institute of Water and Atmospheric Research (NIWA). Thank you to Elena Kupriyanova at the Australian Museum Research Institute and to Glenn Moore and Andrew Hosie at the Western Australian Museum for taxonomic support. We are also grateful to Christine Shonberg at the Australian Institute for Marine Science in WA for helpful discussions about the sponge C. thoosina and to Ingrid Knapp, Anuschka Faucci and Brian Neved at the University of Hawaii for their time and effort attempting to collect specimens in Hawaii. Thank you to two internal (DoF) reviewers and three anonymous referees for their comments. Cornelia Jaspers received support from the Danish Council for Independent Research (No.600207). This project was financially supported by Chevron Australia and the WA Department of Fisheries.",
year = "2017",
month = "6",
doi = "10.3391/mbi.2017.8.2.09",
language = "English",
volume = "8",
pages = "215--225",
journal = "Management of Biological Invasions",
issn = "1989-8649",
publisher = "Regional Euro-Asian Biological Invasions Centre",
number = "2",

}

TY - JOUR

T1 - Establishment of a taxonomic and molecular reference collection to support the identification of species regulated by the Western Australian Prevention List for Introduced Marine Pests

AU - Dias, P. Joana

AU - Fotedar, Seema

AU - Munoz, Julieta

AU - Hewitt, Matthew J.

AU - Lukehurst, Sherralee

AU - Hourston, Mathew

AU - Wellington, Claire

AU - Duggan, Roger

AU - Bridgwood, Samantha

AU - Massam, Marion

AU - Aitken, Victoria

AU - de Lestang, Paul

AU - McKirdy, Simon

AU - Willan, Richard

AU - Kirkendale, Lisa

AU - Giannetta, Jennifer

AU - Corsini-Foka, Maria

AU - Pothoven, Steve

AU - Gower, Fiona

AU - Viard, Frédérique

AU - Buschbaum, Christian

AU - Scarcella, Giuseppe

AU - Strafella, Pierluigi

AU - Bishop, Melanie J.

AU - Sullivan, Timothy

AU - Buttino, Isabella

AU - Madduppa, Hawis

AU - Huhn, Mareike

AU - Zabin, Chela J.

AU - Bacela-Spychalska, Karolina

AU - Wójcik-Fudalewska, Dagmara

AU - Markert, Alexandra

AU - Maximov, Alexey

AU - Kautsky, Lena

AU - Jaspers, Cornelia

AU - Kotta, Jonne

AU - Pärnoja, Merli

AU - Robledo, Daniel

AU - Tsiamis, Konstantinos

AU - Kuepper, Frithjof C.

AU - Žuljević, Ante

AU - McDonald, Justin I.

AU - Snow, Michael

N1 - We acknowledge field sample collection assistance of the WA Government Department of Fisheries (DoF) Biosecurity Research and Compliance teams in obtaining specimens of IMP present in Australia. We also would like to acknowledge field sampling and cooperation of stakeholders in WA. We are most grateful to everyone in Australia and abroad that provided us with specimens including John Lewis at ES Link Services, Craig Boys and Anthony Fowler at the NSW Department of Primary Industries, Simon Grove and Kirrily Moore at the Department of State Growth in Tasmania, and Matt Koopman at Fishwell Consulting in Australia; Eric A. Hoffman at University of Central Florida, Ximing Guo at Rutgers University, Steve Palumbi at Stanford University, Charles Epifanio at the University of Delaware, Toni Renee Ignoffo and Wim Kimmerer at San Francisco State University, Joseph Pawlik and Jack Cushman Koch at the University of North Carolina Wilmington and Kimberly S. Reece at the Virginia Institute of Marine Science, USA; Keith Hiscock at Plymouth Marine Biological Association and Elizabeth Cook at the Scottish Association for Marine Science, UK; Per Jonsson and Gunnar Cervin at the University of Gothenburg and Ann-Britt Florin at the Swedish University of Agricultural Sciences, Sweden; Bente Edvardsen and Wenche Eikrem at the University of Oslo, Norway; Cynthia McKenzie at the Department of Fisheries and Oceans Canada; Annick Verween at Ghent University, Belgium; Nathalie Cochennec-Laureau at IFREMER, France; Bartlomiej Arciszewski at the University of Gdansk, Poland; Marcus Anders Krag and Peter Rask Moller at the Natural History Museum of Denmark; Taeko Kimura at Mie University, Japan; Xiao Liu at the Institute of Oceanology in Qingdao, China; Tsang Ling Ming at National Taiwan Ocean University; Ho Young Soh at Chonnam National University, Republic of Korea; Ronaldo Sousa at University of Minho and Alexandra Teodosio at the University of Algarve, Portugal. We would like to acknowledge the most valuable ongoing support of John Huisman at the Western Australian Herbarium and Serena Wilkens and Mike Page at the New Zealand Marine Invasive Taxonomic Service (MITS) and the National Institute of Water and Atmospheric Research (NIWA). Thank you to Elena Kupriyanova at the Australian Museum Research Institute and to Glenn Moore and Andrew Hosie at the Western Australian Museum for taxonomic support. We are also grateful to Christine Shonberg at the Australian Institute for Marine Science in WA for helpful discussions about the sponge C. thoosina and to Ingrid Knapp, Anuschka Faucci and Brian Neved at the University of Hawaii for their time and effort attempting to collect specimens in Hawaii. Thank you to two internal (DoF) reviewers and three anonymous referees for their comments. Cornelia Jaspers received support from the Danish Council for Independent Research (No.600207). This project was financially supported by Chevron Australia and the WA Department of Fisheries.

PY - 2017/6

Y1 - 2017/6

N2 - Introduced Marine Pests (IMP, = non-indigenous marine species) prevention, early detection and risk-based management strategies have become the priority for biosecurity operations worldwide, in recognition of the fact that, once established, the effective management of marine pests can rapidly become cost prohibitive or impractical. In Western Australia (WA), biosecurity management is guided by the “Western Australian Prevention List for Introduced Marine Pests” which is a policy tool that details species or genera as being of high risk to the region. This list forms the basis of management efforts to prevent introduction of these species, monitoring efforts to detect them at an early stage, and rapid response should they be detected. It is therefore essential that the species listed can be rapid and confidently identified and discriminated from native species by a range of government and industry stakeholders. Recognising that identification of these species requires very specialist expertise which may be in short supply and not readily accessible in a regulatory environment, and the fact that much publicly available data is not verifiable or suitable for regulatory enforcement, the WA government commissioned the current project to collate a reference collection of these marine pest specimens. In this work, we thus established collaboration with researchers worldwide in order to source representative specimens of the species listed. Our main objective was to build a reference collection of taxonomically vouchered specimens and subsequently to generate species-specific DNA barcodes suited to supporting their future identification. To date, we were able to obtain specimens of 75 species (representative of all but four of the pests listed) which have been identified by experts and placed with the WA Government Department of Fisheries and, where possible, in accessible museums and institutions in Australasia. The reference collection supports the fast and reliable taxonomic and molecular identification of marine pests in WA and constitutes a valuable resource for training of stakeholders with interest in IMP recognition in Australia. The reference collection is also useful in supporting the development of a variety of DNA-based detection strategies such as real-time PCR and metabarcoding of complex environmental samples (e.g. biofouling ommunities). The Prevention List is under regular review to ensure its continued relevance and that it remains evidence and risk-based. Similarly, its associated reference collection also remains to some extent a work in progress. In recognition of this fact, this report seeks to provide details of this continually evolving information repository publicly available to the biosecurity management community worldwide.

AB - Introduced Marine Pests (IMP, = non-indigenous marine species) prevention, early detection and risk-based management strategies have become the priority for biosecurity operations worldwide, in recognition of the fact that, once established, the effective management of marine pests can rapidly become cost prohibitive or impractical. In Western Australia (WA), biosecurity management is guided by the “Western Australian Prevention List for Introduced Marine Pests” which is a policy tool that details species or genera as being of high risk to the region. This list forms the basis of management efforts to prevent introduction of these species, monitoring efforts to detect them at an early stage, and rapid response should they be detected. It is therefore essential that the species listed can be rapid and confidently identified and discriminated from native species by a range of government and industry stakeholders. Recognising that identification of these species requires very specialist expertise which may be in short supply and not readily accessible in a regulatory environment, and the fact that much publicly available data is not verifiable or suitable for regulatory enforcement, the WA government commissioned the current project to collate a reference collection of these marine pest specimens. In this work, we thus established collaboration with researchers worldwide in order to source representative specimens of the species listed. Our main objective was to build a reference collection of taxonomically vouchered specimens and subsequently to generate species-specific DNA barcodes suited to supporting their future identification. To date, we were able to obtain specimens of 75 species (representative of all but four of the pests listed) which have been identified by experts and placed with the WA Government Department of Fisheries and, where possible, in accessible museums and institutions in Australasia. The reference collection supports the fast and reliable taxonomic and molecular identification of marine pests in WA and constitutes a valuable resource for training of stakeholders with interest in IMP recognition in Australia. The reference collection is also useful in supporting the development of a variety of DNA-based detection strategies such as real-time PCR and metabarcoding of complex environmental samples (e.g. biofouling ommunities). The Prevention List is under regular review to ensure its continued relevance and that it remains evidence and risk-based. Similarly, its associated reference collection also remains to some extent a work in progress. In recognition of this fact, this report seeks to provide details of this continually evolving information repository publicly available to the biosecurity management community worldwide.

KW - Introduced Marine Pests (IMP)

KW - taxonomic voucher

KW - cytochrome c oxidase I

KW - COI

KW - The Barcode of Life Data System

KW - BoLD

KW - marine biosecurity

U2 - 10.3391/mbi.2017.8.2.09

DO - 10.3391/mbi.2017.8.2.09

M3 - Article

VL - 8

SP - 215

EP - 225

JO - Management of Biological Invasions

JF - Management of Biological Invasions

SN - 1989-8649

IS - 2

ER -