Estradiol stimulates growth hormone production in female goldfish

Jun Zou, V L Trudeau, Z. Cui, J Brechin, Kevin Scott MacKenzie, Z. Zhu, Dominic Houlihan, R.E. Peter

Research output: Contribution to journalArticle

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Abstract

The effects of estradiol (E2) on growth hormone (GH) production was investigated in gonad-intact female goldfish. It was first necessary to generate a specific antibody for use in immunocytochemistry, Western, and dot-blot analyses of GH production. To accomplish this, grass carp GH (gcGH) cDNA was cloned by the reverse transcription polymerase chain reaction (RT-PCR) and expressed in Echerichia coli and a specific polyclonal antibody to recombinant gcGH was generated in the rabbit. In Western blot, the anti-gcGH antibody specifically immunoreacted with recombinant gcGH, purified natural common carp GH, and with a single 21.5-kDa GH form from pituitary extracts of grass carp, common carp, goldfish, and zebrafish but not salmon, trout, or tilapia. Intraperitoneal injection of the recombinant gcGH enhanced the growth rates of juvenile common carp demonstrating biological activity of this GH preparation. Electron microscopic studies showed that the anti-gcGH-I antibody specifically reacted with GH localized in the secretory granules of the goldfish somatotroph. Using anti-gcGH-I in a dot-blot assay, it was found that in vivo implantation of solid silastic pellets containing E2, (100 micrograms/g body weight for 5 days) increased pituitary GH content by 150% in female goldfish. In a second, independent study employing a previously characterized anticommon carp GH antibody for radioimmunoassay, it was found that E2 increased pituitary GH content by 170% and serum GH levels by approximately 350%. The E2-induced hypersecretion of GH and increase in pituitary GH levels was not associated with changes in steady-state pituitary GH mRNA levels, suggesting that this sex steroid may enhance GH synthesis at the posttranscriptional or translational level. Previous observations indicate that GH can stimulate ovarian E2 production. The present results show that E2 can in turn stimulate GH production, indicating the existence of a novel pituitary GH-ovarian feedback system in goldfish.
Original languageEnglish
Pages (from-to)102-112
Number of pages11
JournalGeneral and Comparative Endocrinology
Volume106
Issue number1
DOIs
Publication statusPublished - Apr 1997

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Goldfish
goldfish
somatotropin
Growth Hormone
Carps
estradiol
Estradiol
Ctenopharyngodon idella
Cyprinus carpio
Antibodies
antibodies
Western Blotting
Somatotrophs
hypersecretion
Tilapia
Trout
Salmon
secretory granules
Secretory Vesicles
Gonads

Cite this

Zou, J., Trudeau, V. L., Cui, Z., Brechin, J., MacKenzie, K. S., Zhu, Z., ... Peter, R. E. (1997). Estradiol stimulates growth hormone production in female goldfish. General and Comparative Endocrinology, 106(1), 102-112. https://doi.org/10.1006/gcen.1996.6857

Estradiol stimulates growth hormone production in female goldfish. / Zou, Jun; Trudeau, V L ; Cui, Z. ; Brechin, J; MacKenzie, Kevin Scott; Zhu, Z.; Houlihan, Dominic; Peter, R.E.

In: General and Comparative Endocrinology, Vol. 106, No. 1, 04.1997, p. 102-112.

Research output: Contribution to journalArticle

Zou, J, Trudeau, VL, Cui, Z, Brechin, J, MacKenzie, KS, Zhu, Z, Houlihan, D & Peter, RE 1997, 'Estradiol stimulates growth hormone production in female goldfish', General and Comparative Endocrinology, vol. 106, no. 1, pp. 102-112. https://doi.org/10.1006/gcen.1996.6857
Zou, Jun ; Trudeau, V L ; Cui, Z. ; Brechin, J ; MacKenzie, Kevin Scott ; Zhu, Z. ; Houlihan, Dominic ; Peter, R.E. / Estradiol stimulates growth hormone production in female goldfish. In: General and Comparative Endocrinology. 1997 ; Vol. 106, No. 1. pp. 102-112.
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