Abstract
Objective
The plant Terminalia ivorensis is used in traditional medicine as a diuretic and in the management of renal failure. We reported previously that the ethanol stem bark extract of the plant protects against gentamicin-induced renal and hepatic damage in rats. To further elucidate the mechanism of its renoprotective activity, we studied the effects of the extract on Potassium dichromate–induced nephrotoxicity in rats. The present study assessed the effectiveness of the ethanol stem bark extract of Terminalia ivorensis - against renal oxidative injury evoked by potassium dichromate.
Methods
Adult Sprague Dawley rats pre-treated with (100–1000 mg/kg p.o. bwt) of Terminalia ivorensis extract for 5 days were challenged with a single dose of Potassium dichromate (20 mg/kg Sc) in the neck region on the 4th day. On the sixth day, renal function and markers of oxidative injury were assessed.
Results
Terminalia ivorensis (300–1000 mg/kg p.o) pre-treatment dose dependently prevented decreases in urine output in rats challenged with a nephrotoxic dose of Potassium Dichromate. The extract also protected the rats against Potassium dichromate-induced rise in serum electrolytes, urea and creatinine. Furthermore, it dose dependently prevented Potassium dichromate-induced decrease in renal glutathione (GSH) levels whereas tissue oxidative enzymes Superoxide dismutase (SOD) and catalase were protected from damage. Markers of lipid peroxidation such as level of renal Malondialdehyde (MDA) and myeloperoxidase (MPO) also decreased dose dependently when compare with Potassium dichromate treated groups. The extract also protected the histomorphology of the kidney against Potassium dichromate induced damage.
Conclusion
The ethanol stem bark extract of Terminalia ivorensis protects kidney against Potassium dichromate-induced renal damage.
The plant Terminalia ivorensis is used in traditional medicine as a diuretic and in the management of renal failure. We reported previously that the ethanol stem bark extract of the plant protects against gentamicin-induced renal and hepatic damage in rats. To further elucidate the mechanism of its renoprotective activity, we studied the effects of the extract on Potassium dichromate–induced nephrotoxicity in rats. The present study assessed the effectiveness of the ethanol stem bark extract of Terminalia ivorensis - against renal oxidative injury evoked by potassium dichromate.
Methods
Adult Sprague Dawley rats pre-treated with (100–1000 mg/kg p.o. bwt) of Terminalia ivorensis extract for 5 days were challenged with a single dose of Potassium dichromate (20 mg/kg Sc) in the neck region on the 4th day. On the sixth day, renal function and markers of oxidative injury were assessed.
Results
Terminalia ivorensis (300–1000 mg/kg p.o) pre-treatment dose dependently prevented decreases in urine output in rats challenged with a nephrotoxic dose of Potassium Dichromate. The extract also protected the rats against Potassium dichromate-induced rise in serum electrolytes, urea and creatinine. Furthermore, it dose dependently prevented Potassium dichromate-induced decrease in renal glutathione (GSH) levels whereas tissue oxidative enzymes Superoxide dismutase (SOD) and catalase were protected from damage. Markers of lipid peroxidation such as level of renal Malondialdehyde (MDA) and myeloperoxidase (MPO) also decreased dose dependently when compare with Potassium dichromate treated groups. The extract also protected the histomorphology of the kidney against Potassium dichromate induced damage.
Conclusion
The ethanol stem bark extract of Terminalia ivorensis protects kidney against Potassium dichromate-induced renal damage.
| Original language | English |
|---|---|
| Article number | e00410 |
| Number of pages | 9 |
| Journal | Scientific Africa |
| Volume | 8 |
| Early online date | 20 Jun 2020 |
| DOIs | |
| Publication status | Published - Jul 2020 |
Bibliographical note
Authors are grateful to Mr. Thomas Ansah and Mr. Clifford Asare, KNUST Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Science, Kwame Nkrumah University of Science and Technology, and Tracey Willox and Savannah Jacobs of School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen,UK for their technical support.Funding
Authors had no funding
Keywords
- Nephroprotection
- Oxidative stress
- Glutathione
- Superoxide dismutase
- Traditional medicine