ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis

JJ Rochford, RK Semple, M Laudes, KB Boyle, C Christodoulides, C Mulligan, CJ Lelliott, S Schinner, D Hadaschik, M Mahadevan, JK Sethi, A Vidal-Puig, S O'Rahilly*

*Corresponding author for this work

    Research output: Contribution to journalArticle

    57 Citations (Scopus)

    Abstract

    The putative transcriptional corepressor ETO/MTG8 has been extensively studied due to its involvement in a chromosomal translocation causing the t(8;21) form of acute myeloid leukemia. Despite this, the role of ETO in normal physiology has remained obscure. Here we show that ETO is highly expressed in preadipocytes and acts as an inhibitor of C/EBPbeta during early adipogenesis, contributing to its characteristically delayed activation. ETO prevents both the transcriptional activation of the C/EBPalpha promoter by C/EBPbeta and its concurrent accumulation in centromeric sites during early adipogenesis. ETO expression rapidly reduces after the initiation of adipogenesis, and this is essential to the normal induction of adipogenic gene expression. These findings define, for the first time, a molecular role for ETO in normal physiology as an inhibitor of C/EBPbeta and a novel regulator of early adipogenesis.

    Original languageEnglish
    Pages (from-to)9863-9872
    Number of pages10
    JournalMolecular and Cellular Biology
    Volume24
    Issue number22
    DOIs
    Publication statusPublished - Nov 2004

    Keywords

    • CCAAT/ENHANCER-BINDING PROTEINS
    • RECEPTOR GAMMA-2 PROMOTER
    • ADIPOCYTE DIFFERENTIATION
    • 3T3-L1 PREADIPOCYTES
    • INSULIN SENSITIVITY
    • FUSION PROTEIN
    • GENE PROMOTER
    • GROWTH-FACTOR
    • MICE LACKING
    • ALPHA-GENE

    Cite this

    Rochford, JJ., Semple, RK., Laudes, M., Boyle, KB., Christodoulides, C., Mulligan, C., Lelliott, CJ., Schinner, S., Hadaschik, D., Mahadevan, M., Sethi, JK., Vidal-Puig, A., & O'Rahilly, S. (2004). ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis. Molecular and Cellular Biology, 24(22), 9863-9872. https://doi.org/10.1128/MCB.24.22.9863-9872.2004