Evaluation of Loading Strategies to Improve Tumor Uptake of Gemcitabine in a Murine Orthotopic Bladder Cancer Model Using Ultrasound and Microbubbles

Jia Ling Ruan, Richard J. Browning, Yesna O. Yildiz, Luca Bau, Sukanta Kamila, Michael D. Gray, Lisa Folkes, Alix Hampson, Anthony P. McHale, John F. Callan, Borivoj Vojnovic, Anne E. Kiltie, Eleanor Stride*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In this study we compared three different microbubble-based approaches to the delivery of a widely used chemotherapy drug, gemcitabine: (i) co-administration of gemcitabine and microbubbles (Gem+MB); (ii) conjugates of microbubbles and gemcitabine-loaded liposomes (GemlipoMB); and (iii) microbubbles with gemcitabine directly bound to their surfaces (GembioMB). Both in vitro and in vivo investigations were carried out, respectively, in the RT112 bladder cancer cell line and in a murine orthotopic muscle-invasive bladder cancer model. The in vitro (in vivo) ultrasound exposure conditions were a 1 (1.1) MHz centre frequency, 0.07 (1.0) MPa peak negative pressure, 3000 (20,000) cycles and 100 (0.5) Hz pulse repetition frequency. Ultrasound exposure produced no significant increase in drug uptake either in vitro or in vivo compared with the drug-only control for co-administered gemcitabine and microbubbles. In vivo, GemlipoMB prolonged the plasma circulation time of gemcitabine, but only GembioMB produced a statistically significant increase in cleaved caspase 3 expression in the tumor, indicative of gemcitabine-induced apoptosis.

Original languageEnglish
Pages (from-to)1596-1615
Number of pages20
JournalUltrasound in Medicine and Biology
Volume47
Issue number6
Early online date8 Mar 2021
DOIs
Publication statusPublished - 1 Jun 2021

Keywords

  • Biotinylation
  • Bladder cancer
  • Gemcitabine
  • Liposome
  • Microbubbles
  • Nanoparticles
  • Orthotopic model
  • Ultrasound-mediated drug delivery

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