Evaluation of Maize Streak Virus Pathogenicity in Differentially Resistant Zea mays Genotypes

D P Martin, J A Willment, E P Rybicki

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

ABSTRACT We devised a rapid technique for the objective and precise assessment of both the pathogenicity of maize streak virus (MSV) isolates and the MSV resistance of maize genotypes. The technique involves the use of agroinoculation to infect maize seedlings and the objective symptom evaluation by quantification of infection rates, stunting, and chlorotic leaf areas. In assessing the MSV resistance of 19 maize genotypes, we describe how the use of differentially virulent virus isolates enables the analysis of MSV resistance phenotypes, ranging from extremely susceptible to completely immune. We further demonstrate how quantification of chlorotic leaf areas by image analysis permits differentiation between degrees of MSV resistance that are indistinguishable from one another using currently employed symptom assessment approaches. Using chlorotic area measurements, we quantify the virulence of a diverse group of 10 MSV isolates and, through agroinoculation of differentially susceptible maize genotypes, we demonstrate the use of our technique in evaluating the pathogenicity of these isolates.
Original languageEnglish
Pages (from-to)695-700
Number of pages6
JournalPhytopathology
Volume89
Issue number8
DOIs
Publication statusPublished - 1999

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Maize streak virus
pathogenicity
Zea mays
genotype
corn
signs and symptoms (plants)
leaf area
growth retardation
virulence
methodology
image analysis
phenotype
viruses
seedlings
infection

Cite this

Evaluation of Maize Streak Virus Pathogenicity in Differentially Resistant Zea mays Genotypes. / Martin, D P; Willment, J A; Rybicki, E P.

In: Phytopathology, Vol. 89, No. 8, 1999, p. 695-700.

Research output: Contribution to journalArticle

Martin, D P ; Willment, J A ; Rybicki, E P. / Evaluation of Maize Streak Virus Pathogenicity in Differentially Resistant Zea mays Genotypes. In: Phytopathology. 1999 ; Vol. 89, No. 8. pp. 695-700.
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