Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils: A review

H. Wallander, A. Ekblad, D.L. Godbold, D. Johnson, A. Bahr, P. Baldrian, R.G. Björk, B. Kieliszewska-Rokicka, R. Kjøller, H. Kraigher, C. Plassard, M. Rudawska

Research output: Contribution to journalArticle

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Abstract

Mycorrhizal fungi constitute a considerable sink for carbon in most ecosystems. This carbon is used for building extensive mycelial networks in the soil as well as for metabolic activity related to nutrient uptake. A number of methods have been developed recently to quantify production, standing biomass and turnover of extramatrical mycorrhizal mycelia (EMM) in the field. These methods include minirhizotrons, in-growth mesh bags and cores, and indirect measurements of EMM based on classification of ectomycorrhizal fungi into exploration types. Here we review the state of the art of this methodology and discuss how it can be developed and applied most effectively in the field. Furthermore, we also discuss different ways to quantify fungal biomass based on biomarkers such as chitin, ergosterol and PLFAs, as well as molecular methods, such as qPCR. The evidence thus far indicates that mycorrhizal fungi are key components of microbial biomass in many ecosystems. We highlight the need to extend the application of current methods to focus on a greater range of habitats and mycorrhizal types enabling incorporation of mycorrhizal fungal biomass and turnover into biogeochemical cycling models.
Original languageEnglish
Pages (from-to)1034-1047
Number of pages14
JournalSoil Biology and Biochemistry
Volume57
Early online date10 Sep 2012
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Mycelium
Biomass
forest soils
forest soil
mycelium
biomass production
turnover
Soil
Ecosystem
Fungi
biomass
fungus
Carbon Sequestration
mycorrhizal fungi
Ergosterol
ergosterol
Chitin
chitin
ecosystem
carbon

Keywords

  • chitin
  • exploration type
  • ergosterol
  • extramatrical mycelium
  • in-growth bag
  • minirhizotrin
  • PLFA
  • rizomorphs
  • sampling design
  • turnover rates

Cite this

Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils : A review. / Wallander, H.; Ekblad, A.; Godbold, D.L.; Johnson, D.; Bahr, A.; Baldrian, P.; Björk, R.G.; Kieliszewska-Rokicka, B.; Kjøller, R.; Kraigher, H.; Plassard, C.; Rudawska, M.

In: Soil Biology and Biochemistry, Vol. 57, 02.2013, p. 1034-1047.

Research output: Contribution to journalArticle

Wallander, H, Ekblad, A, Godbold, DL, Johnson, D, Bahr, A, Baldrian, P, Björk, RG, Kieliszewska-Rokicka, B, Kjøller, R, Kraigher, H, Plassard, C & Rudawska, M 2013, 'Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils: A review' Soil Biology and Biochemistry, vol. 57, pp. 1034-1047. https://doi.org/10.1016/j.soilbio.2012.08.027
Wallander, H. ; Ekblad, A. ; Godbold, D.L. ; Johnson, D. ; Bahr, A. ; Baldrian, P. ; Björk, R.G. ; Kieliszewska-Rokicka, B. ; Kjøller, R. ; Kraigher, H. ; Plassard, C. ; Rudawska, M. / Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils : A review. In: Soil Biology and Biochemistry. 2013 ; Vol. 57. pp. 1034-1047.
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