Evidence for activity-dependent modulation of sensory-terminal excitability in spindles by glutamate release from synaptic-like vesicles

Robert W Banks, Guy Smith Bewick, Christine Richardson, Brian Reid

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Sensory terminals of muscle spindles and similar mechanosensory neurons contain large numbers of 50 nm, "synaptic-like" vesicles (SLVs), about whose role very little is known. Using fluorescence microscopy, immunocytochemistry and electrophysiological recording, we present evidence that SLVs undergo a recycling process, and that they release glutamate that has an autogenic excitatory effect on mechanosensory transduction, probably involving a metabotropic receptor linked to phospholipase D. The rate of recycling of SLVs is activity dependent, at least in part, as shown by an increased rate of destaining of preparations labelled with FMI-43 during high-frequency, small-amplitude vibration. Immunogold labelling showed levels of glutamate-like reactivity in the sensory terminals at least as great as in probable Ia presynaptic terminals in the spinal cord. Exogenously applied glutamate has an excitatory effect on the spindle's response to stretch, which is blocked by 3,5-dihydroxyphenylglycine.
Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalAdvances in Experimental Medicine and Biology
Volume508
Publication statusPublished - 1 Jan 2002

Fingerprint

Synaptic Vesicles
Glutamic Acid
Modulation
Recycling
Cellular Mechanotransduction
Muscle Spindles
Phospholipase D
Fluorescence microscopy
Presynaptic Terminals
Vibration
Fluorescence Microscopy
Labeling
Neurons
Muscle
Spinal Cord
Immunohistochemistry

Keywords

  • Animals
  • Electrophysiology
  • Glutamic Acid
  • Immunohistochemistry
  • Microscopy, Fluorescence
  • Muscle Spindles
  • Rats
  • Sensation
  • Stress, Mechanical
  • Synaptic Vesicles

Cite this

Evidence for activity-dependent modulation of sensory-terminal excitability in spindles by glutamate release from synaptic-like vesicles. / Banks, Robert W; Bewick, Guy Smith; Richardson, Christine; Reid, Brian.

In: Advances in Experimental Medicine and Biology, Vol. 508, 01.01.2002, p. 13-18.

Research output: Contribution to journalArticle

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N2 - Sensory terminals of muscle spindles and similar mechanosensory neurons contain large numbers of 50 nm, "synaptic-like" vesicles (SLVs), about whose role very little is known. Using fluorescence microscopy, immunocytochemistry and electrophysiological recording, we present evidence that SLVs undergo a recycling process, and that they release glutamate that has an autogenic excitatory effect on mechanosensory transduction, probably involving a metabotropic receptor linked to phospholipase D. The rate of recycling of SLVs is activity dependent, at least in part, as shown by an increased rate of destaining of preparations labelled with FMI-43 during high-frequency, small-amplitude vibration. Immunogold labelling showed levels of glutamate-like reactivity in the sensory terminals at least as great as in probable Ia presynaptic terminals in the spinal cord. Exogenously applied glutamate has an excitatory effect on the spindle's response to stretch, which is blocked by 3,5-dihydroxyphenylglycine.

AB - Sensory terminals of muscle spindles and similar mechanosensory neurons contain large numbers of 50 nm, "synaptic-like" vesicles (SLVs), about whose role very little is known. Using fluorescence microscopy, immunocytochemistry and electrophysiological recording, we present evidence that SLVs undergo a recycling process, and that they release glutamate that has an autogenic excitatory effect on mechanosensory transduction, probably involving a metabotropic receptor linked to phospholipase D. The rate of recycling of SLVs is activity dependent, at least in part, as shown by an increased rate of destaining of preparations labelled with FMI-43 during high-frequency, small-amplitude vibration. Immunogold labelling showed levels of glutamate-like reactivity in the sensory terminals at least as great as in probable Ia presynaptic terminals in the spinal cord. Exogenously applied glutamate has an excitatory effect on the spindle's response to stretch, which is blocked by 3,5-dihydroxyphenylglycine.

KW - Animals

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KW - Stress, Mechanical

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JO - Advances in Experimental Medicine and Biology

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