Evidence for differences in the post-transcriptional regulation of rat metallothionein isoforms

M H Vasconcelos, S C Tam, J H Beattie, J E Hesketh

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    The expression of metallothionein (MT)-1 and -2 mRNAs in rat liver following administration of Cd or Cu was investigated using specific oligonucleotides. The specificity was confirmed using a competitive prehybridization assay. Cd injection caused a biphasic induction of both isoform mRNAs, whereas Cu induced a sustained, monophasic response. Analysis of polyribosomal RNA showed that, after both Cd and Cu treatments, the recruitment of MT-1 mRNA into polyribosomes paralleled the increase in transcription, but the increase of polyribosomal MT-2 mRNA was less than that of total MT-2 mRNA. This indicates that not all the MT-2 mRNA induced was translated, suggesting that there is translational control of MT-2 mRNA expression, but not of MT-1 mRNA. This hypothesis was supported by the observation that, after Cu treatment, the induction of MT-1 protein was induced to the same extent as MT-1 mRNA, whereas the total MT protein (MT-1+MT-2) was increased far less (7-fold) than MT-2 mRNA (30-fold).

    Original languageEnglish
    Pages (from-to)665-671
    Number of pages7
    JournalBiochemical Journal
    Volume315
    Publication statusPublished - 15 Apr 1996

    Keywords

    • MESSENGER-RNA
    • HEPATIC METALLOTHIONEIN
    • LIVER
    • INDUCTION
    • COPPER
    • EXPRESSION
    • INJECTION
    • GENES
    • FETAL
    • ACID

    Cite this

    Vasconcelos, M. H., Tam, S. C., Beattie, J. H., & Hesketh, J. E. (1996). Evidence for differences in the post-transcriptional regulation of rat metallothionein isoforms. Biochemical Journal, 315, 665-671.

    Evidence for differences in the post-transcriptional regulation of rat metallothionein isoforms. / Vasconcelos, M H ; Tam, S C ; Beattie, J H ; Hesketh, J E .

    In: Biochemical Journal, Vol. 315, 15.04.1996, p. 665-671.

    Research output: Contribution to journalArticle

    Vasconcelos, MH, Tam, SC, Beattie, JH & Hesketh, JE 1996, 'Evidence for differences in the post-transcriptional regulation of rat metallothionein isoforms', Biochemical Journal, vol. 315, pp. 665-671.
    Vasconcelos MH, Tam SC, Beattie JH, Hesketh JE. Evidence for differences in the post-transcriptional regulation of rat metallothionein isoforms. Biochemical Journal. 1996 Apr 15;315:665-671.
    Vasconcelos, M H ; Tam, S C ; Beattie, J H ; Hesketh, J E . / Evidence for differences in the post-transcriptional regulation of rat metallothionein isoforms. In: Biochemical Journal. 1996 ; Vol. 315. pp. 665-671.
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    abstract = "The expression of metallothionein (MT)-1 and -2 mRNAs in rat liver following administration of Cd or Cu was investigated using specific oligonucleotides. The specificity was confirmed using a competitive prehybridization assay. Cd injection caused a biphasic induction of both isoform mRNAs, whereas Cu induced a sustained, monophasic response. Analysis of polyribosomal RNA showed that, after both Cd and Cu treatments, the recruitment of MT-1 mRNA into polyribosomes paralleled the increase in transcription, but the increase of polyribosomal MT-2 mRNA was less than that of total MT-2 mRNA. This indicates that not all the MT-2 mRNA induced was translated, suggesting that there is translational control of MT-2 mRNA expression, but not of MT-1 mRNA. This hypothesis was supported by the observation that, after Cu treatment, the induction of MT-1 protein was induced to the same extent as MT-1 mRNA, whereas the total MT protein (MT-1+MT-2) was increased far less (7-fold) than MT-2 mRNA (30-fold).",
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    AU - Vasconcelos, M H

    AU - Tam, S C

    AU - Beattie, J H

    AU - Hesketh, J E

    PY - 1996/4/15

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    N2 - The expression of metallothionein (MT)-1 and -2 mRNAs in rat liver following administration of Cd or Cu was investigated using specific oligonucleotides. The specificity was confirmed using a competitive prehybridization assay. Cd injection caused a biphasic induction of both isoform mRNAs, whereas Cu induced a sustained, monophasic response. Analysis of polyribosomal RNA showed that, after both Cd and Cu treatments, the recruitment of MT-1 mRNA into polyribosomes paralleled the increase in transcription, but the increase of polyribosomal MT-2 mRNA was less than that of total MT-2 mRNA. This indicates that not all the MT-2 mRNA induced was translated, suggesting that there is translational control of MT-2 mRNA expression, but not of MT-1 mRNA. This hypothesis was supported by the observation that, after Cu treatment, the induction of MT-1 protein was induced to the same extent as MT-1 mRNA, whereas the total MT protein (MT-1+MT-2) was increased far less (7-fold) than MT-2 mRNA (30-fold).

    AB - The expression of metallothionein (MT)-1 and -2 mRNAs in rat liver following administration of Cd or Cu was investigated using specific oligonucleotides. The specificity was confirmed using a competitive prehybridization assay. Cd injection caused a biphasic induction of both isoform mRNAs, whereas Cu induced a sustained, monophasic response. Analysis of polyribosomal RNA showed that, after both Cd and Cu treatments, the recruitment of MT-1 mRNA into polyribosomes paralleled the increase in transcription, but the increase of polyribosomal MT-2 mRNA was less than that of total MT-2 mRNA. This indicates that not all the MT-2 mRNA induced was translated, suggesting that there is translational control of MT-2 mRNA expression, but not of MT-1 mRNA. This hypothesis was supported by the observation that, after Cu treatment, the induction of MT-1 protein was induced to the same extent as MT-1 mRNA, whereas the total MT protein (MT-1+MT-2) was increased far less (7-fold) than MT-2 mRNA (30-fold).

    KW - MESSENGER-RNA

    KW - HEPATIC METALLOTHIONEIN

    KW - LIVER

    KW - INDUCTION

    KW - COPPER

    KW - EXPRESSION

    KW - INJECTION

    KW - GENES

    KW - FETAL

    KW - ACID

    M3 - Article

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    JO - Biochemical Journal

    JF - Biochemical Journal

    SN - 0264-6021

    ER -