Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy

Daniel S Read, Dan J Woodcock, Norval J C Strachan, Kenneth J Forbes, Frances M Colles, Martin C J Maiden, Felicity Clifton-Hadley, Anne Ridley, Ana Vidal, John Rodgers, Andrew S Whiteley, Samuel K Sheppard

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Closely related bacterial isolates can display divergent phenotypes. This can limit the usefulness of phylogenetic studies for understanding bacterial ecology and evolution. Here, we compare phenotyping based on Raman spectrometric analysis of cellular composition to phylogenetic classification by ribosomal multilocus sequence typing (rMLST) in 108 isolates of the zoonotic pathogens Campylobacter jejuni and C. coli. Automatic relevance determination (ARD) was used to identify informative peaks in the Raman spectra that could be used to distinguish strains in taxonomic and host source groups (species, clade, clonal complex, and isolate source/host). Phenotypic characterization based on Raman spectra showed a degree of agreement with genotypic classification using rMLST, with segregation accuracy between species (83.95%), clade (in C. coli, 98.41%), and, to some extent, clonal complex (86.89% C. jejuni ST-21 and ST-45 complexes) being achieved. This confirmed the utility of Raman spectroscopy for lineage classification and the correlation between genotypic and phenotypic classification. In parallel analysis, relatively distantly related isolates (different clonal complexes) were assigned the correct host origin irrespective of the clonal origin (74.07 to 96.97% accuracy) based upon different Raman peaks. This suggests that the phenotypic characteristics, from which the phenotypic signal is derived, are not fixed by clonal descent but are influenced by the host environment and change as strains move between hosts.
Original languageEnglish
Pages (from-to)965-973
Number of pages9
JournalApplied and Environmental Microbiology
Volume79
Issue number3
Early online date30 Nov 2012
DOIs
Publication statusPublished - Feb 2013

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Multilocus Sequence Typing
Raman spectroscopy
Campylobacter coli
Campylobacter jejuni
Raman Spectrum Analysis
phenotypic plasticity
taxonomy
Zoonoses
phylogenetics
phenotype
Ecology
phylogeny
Phenotype
pathogen
multilocus sequence typing
ecology
pathogens

Cite this

Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy. / Read, Daniel S; Woodcock, Dan J; Strachan, Norval J C; Forbes, Kenneth J; Colles, Frances M; Maiden, Martin C J; Clifton-Hadley, Felicity; Ridley, Anne; Vidal, Ana; Rodgers, John; Whiteley, Andrew S; Sheppard, Samuel K.

In: Applied and Environmental Microbiology, Vol. 79, No. 3, 02.2013, p. 965-973.

Research output: Contribution to journalArticle

Read, DS, Woodcock, DJ, Strachan, NJC, Forbes, KJ, Colles, FM, Maiden, MCJ, Clifton-Hadley, F, Ridley, A, Vidal, A, Rodgers, J, Whiteley, AS & Sheppard, SK 2013, 'Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy', Applied and Environmental Microbiology, vol. 79, no. 3, pp. 965-973. https://doi.org/10.1128/AEM.02521-12
Read, Daniel S ; Woodcock, Dan J ; Strachan, Norval J C ; Forbes, Kenneth J ; Colles, Frances M ; Maiden, Martin C J ; Clifton-Hadley, Felicity ; Ridley, Anne ; Vidal, Ana ; Rodgers, John ; Whiteley, Andrew S ; Sheppard, Samuel K. / Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy. In: Applied and Environmental Microbiology. 2013 ; Vol. 79, No. 3. pp. 965-973.
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