Evidence that the hypothermic response of mice to delta-9-tetrahydrocannabinol is not mediated by changes in thermogenesis in brown adipose-tissue

Roger Guy Pertwee, K Nash, P Trayhurn

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

DELTA-9-Tetrahydrocannabinol (20 mg/kg i.p.) and propranolol (20 and 50 mg/kg i.p.) produced marked falls in the rectal temperatures of mice kept at an ambient temperature of 22-degrees-C. Propranolol (50 mg/kg i.p.) also decreased the thermogenic activity of brown fat, as measured by a decrease in the level of [H-3]GDP binding to mitochondria obtained from mouse interscapular brown adipose tissue. In contrast, DELTA-9-tetrahydrocannabinol (20 mg/kg i.p.) did not affect mitochondrial GDP binding even though the dose used was one shown previously to depress heat production. GDP binding was also unaffected by this cannabinoid in brown adipose tissue taken from mice that had been kept at 13-degrees-C instead of 22-degrees-C. In mice kept at 34-degrees-C, isoprenaline (0.25 and 1.0 mg/kg s.c.) induced a marked rise in rectal temperature and increased the level of GDP binding to brown fat mitochondria. Propranolol (50 mg/kg i.p.) prevented the hyperthermic response to isoprenaline, the mice becoming hypothermic instead. DELTA-9-Tetrahydrocannabinol (20 mg/kg i.p.) had no effect on isoprenaline-induced hyperthermia. We conclude from these data that there is no significant involvement of brown adipose tissue in the hypothermic response of mice to DELTA-9-tetrahydrocannabinol.

Original languageEnglish
Pages (from-to)767-770
Number of pages4
JournalCanadian Journal of Physiology and Pharmacology
Volume69
Issue number6
Publication statusPublished - Jun 1991

Keywords

  • DELTA-9-TETRAHYDROCANNABINOL
  • BODY TEMPERATURE
  • BROWN ADIPOSE TISSUE
  • GDP BINDING
  • THERMOGENESIS
  • OXYGEN-CONSUMPTION
  • delta-9-tetrahydrocannabinol
  • body temperature
  • brown adipose-tissue
  • GDP binding
  • thermogenesis

Cite this

@article{9a542d28e7d14acdbb0a47c9f289a76c,
title = "Evidence that the hypothermic response of mice to delta-9-tetrahydrocannabinol is not mediated by changes in thermogenesis in brown adipose-tissue",
abstract = "DELTA-9-Tetrahydrocannabinol (20 mg/kg i.p.) and propranolol (20 and 50 mg/kg i.p.) produced marked falls in the rectal temperatures of mice kept at an ambient temperature of 22-degrees-C. Propranolol (50 mg/kg i.p.) also decreased the thermogenic activity of brown fat, as measured by a decrease in the level of [H-3]GDP binding to mitochondria obtained from mouse interscapular brown adipose tissue. In contrast, DELTA-9-tetrahydrocannabinol (20 mg/kg i.p.) did not affect mitochondrial GDP binding even though the dose used was one shown previously to depress heat production. GDP binding was also unaffected by this cannabinoid in brown adipose tissue taken from mice that had been kept at 13-degrees-C instead of 22-degrees-C. In mice kept at 34-degrees-C, isoprenaline (0.25 and 1.0 mg/kg s.c.) induced a marked rise in rectal temperature and increased the level of GDP binding to brown fat mitochondria. Propranolol (50 mg/kg i.p.) prevented the hyperthermic response to isoprenaline, the mice becoming hypothermic instead. DELTA-9-Tetrahydrocannabinol (20 mg/kg i.p.) had no effect on isoprenaline-induced hyperthermia. We conclude from these data that there is no significant involvement of brown adipose tissue in the hypothermic response of mice to DELTA-9-tetrahydrocannabinol.",
keywords = "DELTA-9-TETRAHYDROCANNABINOL, BODY TEMPERATURE, BROWN ADIPOSE TISSUE, GDP BINDING, THERMOGENESIS, OXYGEN-CONSUMPTION, delta-9-tetrahydrocannabinol, body temperature, brown adipose-tissue, GDP binding, thermogenesis",
author = "Pertwee, {Roger Guy} and K Nash and P Trayhurn",
year = "1991",
month = "6",
language = "English",
volume = "69",
pages = "767--770",
journal = "Canadian Journal of Physiology and Pharmacology",
issn = "0008-4212",
publisher = "National Research Council of Canada",
number = "6",

}

TY - JOUR

T1 - Evidence that the hypothermic response of mice to delta-9-tetrahydrocannabinol is not mediated by changes in thermogenesis in brown adipose-tissue

AU - Pertwee, Roger Guy

AU - Nash, K

AU - Trayhurn, P

PY - 1991/6

Y1 - 1991/6

N2 - DELTA-9-Tetrahydrocannabinol (20 mg/kg i.p.) and propranolol (20 and 50 mg/kg i.p.) produced marked falls in the rectal temperatures of mice kept at an ambient temperature of 22-degrees-C. Propranolol (50 mg/kg i.p.) also decreased the thermogenic activity of brown fat, as measured by a decrease in the level of [H-3]GDP binding to mitochondria obtained from mouse interscapular brown adipose tissue. In contrast, DELTA-9-tetrahydrocannabinol (20 mg/kg i.p.) did not affect mitochondrial GDP binding even though the dose used was one shown previously to depress heat production. GDP binding was also unaffected by this cannabinoid in brown adipose tissue taken from mice that had been kept at 13-degrees-C instead of 22-degrees-C. In mice kept at 34-degrees-C, isoprenaline (0.25 and 1.0 mg/kg s.c.) induced a marked rise in rectal temperature and increased the level of GDP binding to brown fat mitochondria. Propranolol (50 mg/kg i.p.) prevented the hyperthermic response to isoprenaline, the mice becoming hypothermic instead. DELTA-9-Tetrahydrocannabinol (20 mg/kg i.p.) had no effect on isoprenaline-induced hyperthermia. We conclude from these data that there is no significant involvement of brown adipose tissue in the hypothermic response of mice to DELTA-9-tetrahydrocannabinol.

AB - DELTA-9-Tetrahydrocannabinol (20 mg/kg i.p.) and propranolol (20 and 50 mg/kg i.p.) produced marked falls in the rectal temperatures of mice kept at an ambient temperature of 22-degrees-C. Propranolol (50 mg/kg i.p.) also decreased the thermogenic activity of brown fat, as measured by a decrease in the level of [H-3]GDP binding to mitochondria obtained from mouse interscapular brown adipose tissue. In contrast, DELTA-9-tetrahydrocannabinol (20 mg/kg i.p.) did not affect mitochondrial GDP binding even though the dose used was one shown previously to depress heat production. GDP binding was also unaffected by this cannabinoid in brown adipose tissue taken from mice that had been kept at 13-degrees-C instead of 22-degrees-C. In mice kept at 34-degrees-C, isoprenaline (0.25 and 1.0 mg/kg s.c.) induced a marked rise in rectal temperature and increased the level of GDP binding to brown fat mitochondria. Propranolol (50 mg/kg i.p.) prevented the hyperthermic response to isoprenaline, the mice becoming hypothermic instead. DELTA-9-Tetrahydrocannabinol (20 mg/kg i.p.) had no effect on isoprenaline-induced hyperthermia. We conclude from these data that there is no significant involvement of brown adipose tissue in the hypothermic response of mice to DELTA-9-tetrahydrocannabinol.

KW - DELTA-9-TETRAHYDROCANNABINOL

KW - BODY TEMPERATURE

KW - BROWN ADIPOSE TISSUE

KW - GDP BINDING

KW - THERMOGENESIS

KW - OXYGEN-CONSUMPTION

KW - delta-9-tetrahydrocannabinol

KW - body temperature

KW - brown adipose-tissue

KW - GDP binding

KW - thermogenesis

M3 - Article

VL - 69

SP - 767

EP - 770

JO - Canadian Journal of Physiology and Pharmacology

JF - Canadian Journal of Physiology and Pharmacology

SN - 0008-4212

IS - 6

ER -