Evidence that the production of acetate in rat hepatocytes is a predominantly cytoplasmic process

B Crabtree, M J Souter, Susan Elizabeth Anderson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.
Original languageEnglish
Pages (from-to)673-678
Number of pages6
JournalBiochemical Journal
Volume257
Issue number3
DOIs
Publication statusPublished - 1 Feb 1989

Fingerprint

Rats
Hepatocytes
Acetates
Butyrates
Acetyl-CoA Hydrolase
Fluxes
Acetyl Coenzyme A
Cytoplasm
Fatty Acids
Adenosine Triphosphate
Substrates

Keywords

  • Acetates
  • Acetyl Coenzyme A
  • Acetyl-CoA Hydrolase
  • Animals
  • Butyric Acid
  • Butyric Acids
  • Citrates
  • Cytoplasm
  • Fatty Acids
  • Hydrolysis
  • Liver
  • Rats

Cite this

Evidence that the production of acetate in rat hepatocytes is a predominantly cytoplasmic process. / Crabtree, B; Souter, M J; Anderson, Susan Elizabeth.

In: Biochemical Journal, Vol. 257, No. 3, 01.02.1989, p. 673-678.

Research output: Contribution to journalArticle

Crabtree, B ; Souter, M J ; Anderson, Susan Elizabeth. / Evidence that the production of acetate in rat hepatocytes is a predominantly cytoplasmic process. In: Biochemical Journal. 1989 ; Vol. 257, No. 3. pp. 673-678.
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AB - By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.

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KW - Animals

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KW - Butyric Acids

KW - Citrates

KW - Cytoplasm

KW - Fatty Acids

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KW - Liver

KW - Rats

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