Evidence that the production of acetate in rat hepatocytes is a predominantly cytoplasmic process

B Crabtree, M J Souter, Susan Elizabeth Anderson

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By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.
Original languageEnglish
Pages (from-to)673-678
Number of pages6
JournalBiochemical Journal
Issue number3
Publication statusPublished - 1 Feb 1989



  • Acetates
  • Acetyl Coenzyme A
  • Acetyl-CoA Hydrolase
  • Animals
  • Butyric Acid
  • Butyric Acids
  • Citrates
  • Cytoplasm
  • Fatty Acids
  • Hydrolysis
  • Liver
  • Rats

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