Abstract
By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.
Original language | English |
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Pages (from-to) | 673-678 |
Number of pages | 6 |
Journal | Biochemical Journal |
Volume | 257 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Feb 1989 |
Keywords
- Acetates
- Acetyl Coenzyme A
- Acetyl-CoA Hydrolase
- Animals
- Butyric Acid
- Butyric Acids
- Citrates
- Cytoplasm
- Fatty Acids
- Hydrolysis
- Liver
- Rats