Evolution of follistatin in teleosts revealed through phylogenetic, genomic and expression analyses

Daniel J Macqueen, Ian A Johnston

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Follistatin (Fst) inhibits transforming growth factor-beta (TGF-B) proteins and is a known regulator of amniote myogenesis. Here, we used phylogenetic, genomic and experimental approaches to study its evolution in teleosts. Phylogenetic analyses suggested that one fst gene (fst1) is common to euteleosts, but a second gene (fst2) is conserved specifically within the Ostariophysi. Zebrafish fst1/2 respectively appear on chromosomes 5 and 10 in two genomic regions, each with conserved synteny to a single region in tetrapods. Interestingly, other teleosts have two corresponding chromosomal regions with a similar repertoire of paralogues. Phylogenetic reconstruction clustered these gene duplicates into two sister clades branching from tetrapod sequences. We suggest that an ancestral fst-containing chromosome was duplicated during the teleost whole genome duplication, but that fst2 was lost in lineages external to the Ostariophysi. We show that Fst1 of teleosts/mammals has evolved under strong purifying selection, but the N-terminal of Fst2 may have evolved under positive selection. Furthermore, the tissue-specific expression of zebrafish fst2 was restricted to fewer tissues compared to its paralogue and the single fst1 orthologue of Atlantic salmon (Salmo salar). Zebrafish fst1/2 may have subfunctionalized relative to non-duplicated vertebrate lineages, as several regions in the fst promoter of tetrapods were conserved with one paralogue, but not both. Finally, we examined the embryonic expression of fst1 in a teleost outside the Ostariophysi (Atlantic salmon). During segmentation, fst1 was expressed in the anterior somite compartment but was excluded from muscle progenitors that strongly expressed myogenic regulatory factors (MRFs). Later, fst1 was expressed in myogenic progenitors of the pectoral fin buds and also within the pax7(+) cell layer external to the myotome.
Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalDevelopment Genes and Evolution
Volume218
Issue number1
Early online date11 Dec 2007
DOIs
Publication statusPublished - Jan 2008

Fingerprint

follistatin
Follistatin
Salmo salar
Zebrafish
teleost
Danio rerio
genomics
tetrapod
phylogenetics
phylogeny
Myogenic Regulatory Factors
Duplicate Genes
chromosomes
Synteny
duplicate genes
Chromosomes, Human, Pair 10
Somites
Chromosomes, Human, Pair 5
Muscle Development
muscle development

Keywords

  • Fst evolution
  • whole genome duplication (WGD)
  • subfunctionalization
  • anterior somite compartment
  • embryonic myogenesis

Cite this

Evolution of follistatin in teleosts revealed through phylogenetic, genomic and expression analyses. / Macqueen, Daniel J; Johnston, Ian A.

In: Development Genes and Evolution, Vol. 218, No. 1, 01.2008, p. 1-14.

Research output: Contribution to journalArticle

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