Evolutionary history of the T cell receptor complex as revealed by small-spotted catshark (Scyliorhinus canicula)

Rita Pettinello; Anthony K Redmond; Christopher J Secombes; Daniel J Macqueen; Helen Dooley

doi:10.1016/j.dci.2017.04.015

Evolutionary history of the T cell receptor complex as revealed by small-spotted catshark (Scyliorhinus canicula)

Rita Pettinello, Anthony K Redmond, Christopher J Secombes, Daniel J Macqueen, Helen Dooley

University of Maryland, College Park

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

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Abstract

In every jawed vertebrate species studied so far, the T cell receptor (TCR) complex is composed of two different TCR chains (α/β or γ/δ) and a number of CD3 subunits responsible for transmitting signals into the T cell. In this study, we characterised all of the TCR and CD3 genes of small-spotted catshark (Scyliorhinus canicula) and analysed their expression in a broad range of tissues. While the TCR complex is highly conserved across jawed vertebrates, we identified a number of differences in catshark, most notably the presence of two copies of both TCRβ and CD3γδ, and the absence of a functionally-important proline rich region from CD3ε. We also demonstrate that TCRβ has duplicated independently multiple times in jawed vertebrate evolution, bringing additional diversity to the TCR complex. This study reveals new insights about the evolutionary history of the TCR complex and raises new avenues for future exploration.

Original language	English
Pages (from-to)	125-135
Number of pages	11
Journal	Developmental and Comparative Immunology
Volume	74
Early online date	19 Apr 2017
DOIs	https://doi.org/10.1016/j.dci.2017.04.015
Publication status	Published - Sept 2017

Bibliographical note

Acknowledgements
This work was supported by a PhD studentship awarded by the University of Aberdeen, College of Life Sciences and Medicine to RP, and a Royal Society research grant [RG130789] awarded to HD. The authors thank Kathryn Crouch for providing the nurse shark transcriptome assembly, Milena Monte, Kimberley Mackenzie, Heather Ritchie and Abdullah Alzaid for technical advice, and Marianna Chimienti for help with manuscript editing.

Keywords

T cell receptor
CD3
Cartilaginous fish
Shark
Evolution

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Accepted Manuscript
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Accepted author manuscript, 2.74 MBLicence: CC BY-NC-ND
Supplementary MaterialsAccepted author manuscript, 1.21 MBLicence: CC BY-NC-ND

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Cite this

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title = "Evolutionary history of the T cell receptor complex as revealed by small-spotted catshark (Scyliorhinus canicula)",

abstract = "In every jawed vertebrate species studied so far, the T cell receptor (TCR) complex is composed of two different TCR chains (α/β or γ/δ) and a number of CD3 subunits responsible for transmitting signals into the T cell. In this study, we characterised all of the TCR and CD3 genes of small-spotted catshark (Scyliorhinus canicula) and analysed their expression in a broad range of tissues. While the TCR complex is highly conserved across jawed vertebrates, we identified a number of differences in catshark, most notably the presence of two copies of both TCRβ and CD3γδ, and the absence of a functionally-important proline rich region from CD3ε. We also demonstrate that TCRβ has duplicated independently multiple times in jawed vertebrate evolution, bringing additional diversity to the TCR complex. This study reveals new insights about the evolutionary history of the TCR complex and raises new avenues for future exploration.",

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author = "Rita Pettinello and Redmond, {Anthony K} and Secombes, {Christopher J} and Macqueen, {Daniel J} and Helen Dooley",

note = "Acknowledgements This work was supported by a PhD studentship awarded by the University of Aberdeen, College of Life Sciences and Medicine to RP, and a Royal Society research grant [RG130789] awarded to HD. The authors thank Kathryn Crouch for providing the nurse shark transcriptome assembly, Milena Monte, Kimberley Mackenzie, Heather Ritchie and Abdullah Alzaid for technical advice, and Marianna Chimienti for help with manuscript editing.",

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language = "English",

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AU - Pettinello, Rita

AU - Redmond, Anthony K

AU - Secombes, Christopher J

AU - Macqueen, Daniel J

AU - Dooley, Helen

N1 - Acknowledgements This work was supported by a PhD studentship awarded by the University of Aberdeen, College of Life Sciences and Medicine to RP, and a Royal Society research grant [RG130789] awarded to HD. The authors thank Kathryn Crouch for providing the nurse shark transcriptome assembly, Milena Monte, Kimberley Mackenzie, Heather Ritchie and Abdullah Alzaid for technical advice, and Marianna Chimienti for help with manuscript editing.

PY - 2017/9

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N2 - In every jawed vertebrate species studied so far, the T cell receptor (TCR) complex is composed of two different TCR chains (α/β or γ/δ) and a number of CD3 subunits responsible for transmitting signals into the T cell. In this study, we characterised all of the TCR and CD3 genes of small-spotted catshark (Scyliorhinus canicula) and analysed their expression in a broad range of tissues. While the TCR complex is highly conserved across jawed vertebrates, we identified a number of differences in catshark, most notably the presence of two copies of both TCRβ and CD3γδ, and the absence of a functionally-important proline rich region from CD3ε. We also demonstrate that TCRβ has duplicated independently multiple times in jawed vertebrate evolution, bringing additional diversity to the TCR complex. This study reveals new insights about the evolutionary history of the TCR complex and raises new avenues for future exploration.

AB - In every jawed vertebrate species studied so far, the T cell receptor (TCR) complex is composed of two different TCR chains (α/β or γ/δ) and a number of CD3 subunits responsible for transmitting signals into the T cell. In this study, we characterised all of the TCR and CD3 genes of small-spotted catshark (Scyliorhinus canicula) and analysed their expression in a broad range of tissues. While the TCR complex is highly conserved across jawed vertebrates, we identified a number of differences in catshark, most notably the presence of two copies of both TCRβ and CD3γδ, and the absence of a functionally-important proline rich region from CD3ε. We also demonstrate that TCRβ has duplicated independently multiple times in jawed vertebrate evolution, bringing additional diversity to the TCR complex. This study reveals new insights about the evolutionary history of the TCR complex and raises new avenues for future exploration.

KW - T cell receptor

KW - CD3

KW - Cartilaginous fish

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KW - Evolution

U2 - 10.1016/j.dci.2017.04.015

DO - 10.1016/j.dci.2017.04.015

M3 - Article

C2 - 28433528

SN - 0145-305X

VL - 74

SP - 125

EP - 135

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

ER -

Evolutionary history of the T cell receptor complex as revealed by small-spotted catshark (Scyliorhinus canicula)

Abstract

Bibliographical note

Keywords

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