Evolutionary relationships among diverse bacteriophages and prophages: All the world's a phage

R W Hendrix, Margaret Caroline MacHin Smith, Nicholas Michael Burns, Isabella Mary Ford, G F Hatfull

Research output: Contribution to journalArticle

583 Citations (Scopus)

Abstract

We report DNA and predicted protein sequence similarities, implying homology, among genes of double-stranded DNA (dsDNA) bacteriophages and prophages spanning a broad phylogenetic range of host bacteria. The sequence matches reported here establish genetic connections, not always direct, among the lambdoid phages of Escherichia coli, phage phi C31 of Streptomyces, phages of Mycobacterium, a previously unrecognized cryptic prophage, phi flu, in the Haemophilus influenzae genome, and two small prophage-like elements, phi Rv1 and phi Rv2, in the genome of Mycobacterium tuberculosis. The results imply that these phage genes, and very possibly all of the dsDNA tailed phages, share common ancestry. We propose a model for the genetic structure and dynamics of the global phage population in which all dsDNA phage genomes are mosaics with access, by horizontal exchange, to a large common genetic pool but in which access to the gene pool is not uniform for all phage.

Original languageEnglish
Pages (from-to)2192-2197
Number of pages6
JournalPNAS
Volume96
Issue number5
Publication statusPublished - 2 Mar 1999

Keywords

  • TAIL FIBER GENES
  • GENOME
  • INTEGRATION
  • SEQUENCES
  • SITE

Cite this

Hendrix, R. W., Smith, M. C. M., Burns, N. M., Ford, I. M., & Hatfull, G. F. (1999). Evolutionary relationships among diverse bacteriophages and prophages: All the world's a phage. PNAS, 96(5), 2192-2197.

Evolutionary relationships among diverse bacteriophages and prophages: All the world's a phage. / Hendrix, R W ; Smith, Margaret Caroline MacHin; Burns, Nicholas Michael; Ford, Isabella Mary; Hatfull, G F .

In: PNAS, Vol. 96, No. 5, 02.03.1999, p. 2192-2197.

Research output: Contribution to journalArticle

Hendrix, RW, Smith, MCM, Burns, NM, Ford, IM & Hatfull, GF 1999, 'Evolutionary relationships among diverse bacteriophages and prophages: All the world's a phage', PNAS, vol. 96, no. 5, pp. 2192-2197.
Hendrix RW, Smith MCM, Burns NM, Ford IM, Hatfull GF. Evolutionary relationships among diverse bacteriophages and prophages: All the world's a phage. PNAS. 1999 Mar 2;96(5):2192-2197.
Hendrix, R W ; Smith, Margaret Caroline MacHin ; Burns, Nicholas Michael ; Ford, Isabella Mary ; Hatfull, G F . / Evolutionary relationships among diverse bacteriophages and prophages: All the world's a phage. In: PNAS. 1999 ; Vol. 96, No. 5. pp. 2192-2197.
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AU - Hatfull, G F

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N2 - We report DNA and predicted protein sequence similarities, implying homology, among genes of double-stranded DNA (dsDNA) bacteriophages and prophages spanning a broad phylogenetic range of host bacteria. The sequence matches reported here establish genetic connections, not always direct, among the lambdoid phages of Escherichia coli, phage phi C31 of Streptomyces, phages of Mycobacterium, a previously unrecognized cryptic prophage, phi flu, in the Haemophilus influenzae genome, and two small prophage-like elements, phi Rv1 and phi Rv2, in the genome of Mycobacterium tuberculosis. The results imply that these phage genes, and very possibly all of the dsDNA tailed phages, share common ancestry. We propose a model for the genetic structure and dynamics of the global phage population in which all dsDNA phage genomes are mosaics with access, by horizontal exchange, to a large common genetic pool but in which access to the gene pool is not uniform for all phage.

AB - We report DNA and predicted protein sequence similarities, implying homology, among genes of double-stranded DNA (dsDNA) bacteriophages and prophages spanning a broad phylogenetic range of host bacteria. The sequence matches reported here establish genetic connections, not always direct, among the lambdoid phages of Escherichia coli, phage phi C31 of Streptomyces, phages of Mycobacterium, a previously unrecognized cryptic prophage, phi flu, in the Haemophilus influenzae genome, and two small prophage-like elements, phi Rv1 and phi Rv2, in the genome of Mycobacterium tuberculosis. The results imply that these phage genes, and very possibly all of the dsDNA tailed phages, share common ancestry. We propose a model for the genetic structure and dynamics of the global phage population in which all dsDNA phage genomes are mosaics with access, by horizontal exchange, to a large common genetic pool but in which access to the gene pool is not uniform for all phage.

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KW - INTEGRATION

KW - SEQUENCES

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