Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110

J. W. Dale; H. Al-Ghusein; P. Butcher; A. L. Dickens; F. Drobniewski; Kenneth James Forbes; S. H. Gillespie; D. Lamprecht; T. D. McHugh; R. Pitman; N. Rastogi; A. T. Smith; C. Sola; Hasan Yesilkaya; S. Al-Hashmi

doi:10.1128/JB.185.8.2555-2562.2003

Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110

J. W. Dale, H. Al-Ghusein, P. Butcher, A. L. Dickens, F. Drobniewski, Kenneth James Forbes, S. H. Gillespie, D. Lamprecht, T. D. McHugh, R. Pitman, N. Rastogi, A. T. Smith, C. Sola, Hasan Yesilkaya, S. Al-Hashmi

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34 Citations (Scopus)

Abstract

Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.

Original language	English
Pages (from-to)	2555-2562
Number of pages	7
Journal	Journal of Bacteriology
Volume	185
Issue number	8
DOIs	https://doi.org/10.1128/JB.185.8.2555-2562.2003
Publication status	Published - 2003

Keywords

REPETITIVE DNA-SEQUENCES
LOW COPY NUMBERS
MOLECULAR EPIDEMIOLOGY
NONRANDOM ASSOCIATION
COMPLEX
INSERTION
GENOME
LONDON
SINGLE
RECOMMENDATIONS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JB.185.8.2555-2562.2003

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Dale, J. W., Al-Ghusein, H., Butcher, P., Dickens, A. L., Drobniewski, F., Forbes, K. J., Gillespie, S. H., Lamprecht, D., McHugh, T. D., Pitman, R., Rastogi, N., Smith, A. T., Sola, C., Yesilkaya, H., & Al-Hashmi, S. (2003). Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110. Journal of Bacteriology, 185(8), 2555-2562. https://doi.org/10.1128/JB.185.8.2555-2562.2003

Dale, JW, Al-Ghusein, H, Butcher, P, Dickens, AL, Drobniewski, F, Forbes, KJ, Gillespie, SH, Lamprecht, D, McHugh, TD, Pitman, R, Rastogi, N, Smith, AT, Sola, C, Yesilkaya, H & Al-Hashmi, S 2003, 'Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110', Journal of Bacteriology, vol. 185, no. 8, pp. 2555-2562. https://doi.org/10.1128/JB.185.8.2555-2562.2003

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title = "Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110",

abstract = "Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.",

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AU - Dale, J. W.

AU - Al-Ghusein, H.

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AU - Dickens, A. L.

AU - Drobniewski, F.

AU - Forbes, Kenneth James

AU - Gillespie, S. H.

AU - Lamprecht, D.

AU - McHugh, T. D.

AU - Pitman, R.

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AU - Sola, C.

AU - Yesilkaya, Hasan

AU - Al-Hashmi, S.

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AB - Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.

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KW - LOW COPY NUMBERS

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KW - INSERTION

KW - GENOME

KW - LONDON

KW - SINGLE

KW - RECOMMENDATIONS

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DO - 10.1128/JB.185.8.2555-2562.2003

M3 - Article

SN - 0021-9193

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EP - 2562

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 8

ER -

Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110

Abstract

Keywords

UN SDGs

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