Exogenous corticosterone restores rhythmic expression of the clock gene, PER2, in the central extended amygdala in adrenalectomized rats

Lauren Segall, Jennifer Perrin, Barry Robinson, Diane Rodaros, Shimon Amir

Research output: Contribution to conferencePoster

Abstract

The presence of circadian oscillators in the central nucleus of the amygdala (CEA) and the oval nucleus of the bed nucleus of the stria terminalis (BNST-OV), structures that are important in stress and homeostatic responses, has recently been revealed using immunocytochemistry for the clock gene protein Period 2 (PER2). Clocks have also been discovered in the basolateral amygdala (BLA) and the hippocampus (HIPP), areas important in learning and memory, and emotion. The circadian master clock in the suprachiasmatic nucleus (SCN) drives the daily corticosterone rhythm, but does not itself have any glucocortocoid receptors and, along with the BLA and HIPP, is unaffected by adrenalectomy. In contrast, the rhythms of PER2 in the BNST-OV and CEA, normally the same as that of the SCN, are severely attenuated by adrenalectomy. To determine the role of the rat adrenal hormone corticosterone (CORT) in modulating rhythmic PER2 expression in these areas, we placed adrenalectomized (ADX) rats on one of two CORT replacement schedules: a subcutaneous 30 day time-release CORT pellet (100mg), which provided constant basal levels of 50ng/ml over a period of two weeks, or a circadian rhythm of CORT, with levels peaking at the onset of activity at an average of 11.058 µg/dl, which was achieved by providing CORT in the drinking water at a concentration of 25mg/l. Results indicate that a CORT rhythm is required for complete restoration of rhythmic PER2 expression in the CEA and BNST-OV, implicating CORT as a potential factor in synchronizing peripheral oscillators.
Original languageEnglish
Publication statusPublished - 2005
EventSociety for Neuroscience Annual Meeting 2005 - Washingtom, United States
Duration: 12 Nov 200516 Nov 2005

Conference

ConferenceSociety for Neuroscience Annual Meeting 2005
CountryUnited States
CityWashingtom
Period12/11/0516/11/05

Fingerprint

Corticosterone
Gene Expression
Suprachiasmatic Nucleus
Adrenalectomy
Hippocampus
Septal Nuclei
Circadian Clocks
Central Amygdaloid Nucleus
Circadian Rhythm
Drinking Water
Appointments and Schedules
Emotions
Immunohistochemistry
Learning
Hormones

Keywords

  • circadian rhythms
  • corticosterone
  • PER2
  • glucocorticoid
  • hippocampus
  • stress

Cite this

Segall, L., Perrin, J., Robinson, B., Rodaros, D., & Amir, S. (2005). Exogenous corticosterone restores rhythmic expression of the clock gene, PER2, in the central extended amygdala in adrenalectomized rats. Poster session presented at Society for Neuroscience Annual Meeting 2005, Washingtom, United States.

Exogenous corticosterone restores rhythmic expression of the clock gene, PER2, in the central extended amygdala in adrenalectomized rats. / Segall, Lauren; Perrin, Jennifer; Robinson, Barry; Rodaros, Diane; Amir, Shimon.

2005. Poster session presented at Society for Neuroscience Annual Meeting 2005, Washingtom, United States.

Research output: Contribution to conferencePoster

Segall, L, Perrin, J, Robinson, B, Rodaros, D & Amir, S 2005, 'Exogenous corticosterone restores rhythmic expression of the clock gene, PER2, in the central extended amygdala in adrenalectomized rats' Society for Neuroscience Annual Meeting 2005, Washingtom, United States, 12/11/05 - 16/11/05, .
Segall L, Perrin J, Robinson B, Rodaros D, Amir S. Exogenous corticosterone restores rhythmic expression of the clock gene, PER2, in the central extended amygdala in adrenalectomized rats. 2005. Poster session presented at Society for Neuroscience Annual Meeting 2005, Washingtom, United States.
Segall, Lauren ; Perrin, Jennifer ; Robinson, Barry ; Rodaros, Diane ; Amir, Shimon. / Exogenous corticosterone restores rhythmic expression of the clock gene, PER2, in the central extended amygdala in adrenalectomized rats. Poster session presented at Society for Neuroscience Annual Meeting 2005, Washingtom, United States.
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AB - The presence of circadian oscillators in the central nucleus of the amygdala (CEA) and the oval nucleus of the bed nucleus of the stria terminalis (BNST-OV), structures that are important in stress and homeostatic responses, has recently been revealed using immunocytochemistry for the clock gene protein Period 2 (PER2). Clocks have also been discovered in the basolateral amygdala (BLA) and the hippocampus (HIPP), areas important in learning and memory, and emotion. The circadian master clock in the suprachiasmatic nucleus (SCN) drives the daily corticosterone rhythm, but does not itself have any glucocortocoid receptors and, along with the BLA and HIPP, is unaffected by adrenalectomy. In contrast, the rhythms of PER2 in the BNST-OV and CEA, normally the same as that of the SCN, are severely attenuated by adrenalectomy. To determine the role of the rat adrenal hormone corticosterone (CORT) in modulating rhythmic PER2 expression in these areas, we placed adrenalectomized (ADX) rats on one of two CORT replacement schedules: a subcutaneous 30 day time-release CORT pellet (100mg), which provided constant basal levels of 50ng/ml over a period of two weeks, or a circadian rhythm of CORT, with levels peaking at the onset of activity at an average of 11.058 µg/dl, which was achieved by providing CORT in the drinking water at a concentration of 25mg/l. Results indicate that a CORT rhythm is required for complete restoration of rhythmic PER2 expression in the CEA and BNST-OV, implicating CORT as a potential factor in synchronizing peripheral oscillators.

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