Exogenous retinoic acid causes specific alterations in the development of the midbrain and hindbrain of the zebrafish embryo including positional respecification of the Mauthner neuron

J Hill, Jonathan D Clarke, Neil Vargesson, T Jowett, N Holder

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Exogenously applied retinoic acid given at the early stages of gastrulation causes abnormal development of the caudal midbrain and anterior hindbrain in vertebrate embryos. We describe the limits of the brain regions that are affected using neuroanatomical criteria in the zebrafish embryo. Analysis of the reticulospinal complex shows that the Mauthner cell, which normally differentiates in rhombomere 4, is duplicated either in this rhombomere or in rhombomere 2. Using probes for zebrafish krx20 and pax2, it is demonstrated that retinoic acid affects the expression domains of these regulatory genes in a manner that is consistent with the neuroanatomical data. Expression of the goosecoid gene, which expressed in the prospective anterior mesoderm from the onset of gastrulation, is unaffected by the doses of retinoic acid used in this study, reflecting the normal development of the anterior end of the embryo.
Original languageEnglish
Pages (from-to)3-16
Number of pages14
JournalMechanisms of Development
Volume50
Issue number1
DOIs
Publication statusPublished - 1 Mar 1995

Fingerprint

Rhombencephalon
Zebrafish
Mesencephalon
Tretinoin
Gastrulation
Embryonic Structures
Neurons
Mesoderm
Regulator Genes
Vertebrates
Gene Expression
Brain

Keywords

  • Animals
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Genes, Regulator
  • Mesencephalon
  • Neurons
  • Phenotype
  • Prosencephalon
  • Reticular Formation
  • Rhombencephalon
  • Spinal Cord
  • Tretinoin
  • Zebrafish
  • Retinoic acid
  • Mauthner cell
  • Rhombomeres

Cite this

@article{8d4df494897e428ba23c1fbbe61afe50,
title = "Exogenous retinoic acid causes specific alterations in the development of the midbrain and hindbrain of the zebrafish embryo including positional respecification of the Mauthner neuron",
abstract = "Exogenously applied retinoic acid given at the early stages of gastrulation causes abnormal development of the caudal midbrain and anterior hindbrain in vertebrate embryos. We describe the limits of the brain regions that are affected using neuroanatomical criteria in the zebrafish embryo. Analysis of the reticulospinal complex shows that the Mauthner cell, which normally differentiates in rhombomere 4, is duplicated either in this rhombomere or in rhombomere 2. Using probes for zebrafish krx20 and pax2, it is demonstrated that retinoic acid affects the expression domains of these regulatory genes in a manner that is consistent with the neuroanatomical data. Expression of the goosecoid gene, which expressed in the prospective anterior mesoderm from the onset of gastrulation, is unaffected by the doses of retinoic acid used in this study, reflecting the normal development of the anterior end of the embryo.",
keywords = "Animals, Embryo, Nonmammalian, Gene Expression Regulation, Developmental, Genes, Regulator, Mesencephalon, Neurons, Phenotype, Prosencephalon, Reticular Formation, Rhombencephalon, Spinal Cord, Tretinoin, Zebrafish, Retinoic acid, Mauthner cell, Rhombomeres",
author = "J Hill and Clarke, {Jonathan D} and Neil Vargesson and T Jowett and N Holder",
year = "1995",
month = "3",
day = "1",
doi = "10.1016/0925-4773(94)00321-D",
language = "English",
volume = "50",
pages = "3--16",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Exogenous retinoic acid causes specific alterations in the development of the midbrain and hindbrain of the zebrafish embryo including positional respecification of the Mauthner neuron

AU - Hill, J

AU - Clarke, Jonathan D

AU - Vargesson, Neil

AU - Jowett, T

AU - Holder, N

PY - 1995/3/1

Y1 - 1995/3/1

N2 - Exogenously applied retinoic acid given at the early stages of gastrulation causes abnormal development of the caudal midbrain and anterior hindbrain in vertebrate embryos. We describe the limits of the brain regions that are affected using neuroanatomical criteria in the zebrafish embryo. Analysis of the reticulospinal complex shows that the Mauthner cell, which normally differentiates in rhombomere 4, is duplicated either in this rhombomere or in rhombomere 2. Using probes for zebrafish krx20 and pax2, it is demonstrated that retinoic acid affects the expression domains of these regulatory genes in a manner that is consistent with the neuroanatomical data. Expression of the goosecoid gene, which expressed in the prospective anterior mesoderm from the onset of gastrulation, is unaffected by the doses of retinoic acid used in this study, reflecting the normal development of the anterior end of the embryo.

AB - Exogenously applied retinoic acid given at the early stages of gastrulation causes abnormal development of the caudal midbrain and anterior hindbrain in vertebrate embryos. We describe the limits of the brain regions that are affected using neuroanatomical criteria in the zebrafish embryo. Analysis of the reticulospinal complex shows that the Mauthner cell, which normally differentiates in rhombomere 4, is duplicated either in this rhombomere or in rhombomere 2. Using probes for zebrafish krx20 and pax2, it is demonstrated that retinoic acid affects the expression domains of these regulatory genes in a manner that is consistent with the neuroanatomical data. Expression of the goosecoid gene, which expressed in the prospective anterior mesoderm from the onset of gastrulation, is unaffected by the doses of retinoic acid used in this study, reflecting the normal development of the anterior end of the embryo.

KW - Animals

KW - Embryo, Nonmammalian

KW - Gene Expression Regulation, Developmental

KW - Genes, Regulator

KW - Mesencephalon

KW - Neurons

KW - Phenotype

KW - Prosencephalon

KW - Reticular Formation

KW - Rhombencephalon

KW - Spinal Cord

KW - Tretinoin

KW - Zebrafish

KW - Retinoic acid

KW - Mauthner cell

KW - Rhombomeres

U2 - 10.1016/0925-4773(94)00321-D

DO - 10.1016/0925-4773(94)00321-D

M3 - Article

C2 - 7605750

VL - 50

SP - 3

EP - 16

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 1

ER -