Exposure to a Human Relevant Mixture of Persistent Organic Pollutants or to Perfluorooctane Sulfonic Acid Alone Dysregulates the Developing Cerebellum of Chicken Embryo

Ajay Yadav* (Corresponding Author), Steven Verhaegen, Panagiotis Filis, Diana Domanska, Robert Lyle, Arvind Y.M. Sundaram, Magnus Leithaug, Gunn Charlotte Østby, Mona Aleksandersen, Hanne Friis Berntsen, Karin Elisabeth Zimmer, Paul Fowler , Ragnhild Elisabeth Paulsen, Erik Ropstad

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Prenatal exposure to persistent organic pollutants (POPs) is associated with neurodevelopmental disorders. In the present study, we explored whether a human-relevant POP mixture affects the development of chicken embryo cerebellum. We used a defined mixture of 29 POPs, with chemical composition and concentrations based on blood levels in the Scandinavian population. We also evaluated exposure to a prominent compound in the mixture, perfluorooctane sulfonic acid (PFOS), alone. Embryos (n=7-9 per exposure group) were exposed by injection directly into the allantois at embryonic day 13 (E13). Cerebella were isolated at E17 and subjected to morphological, RNA-seq and shot-gun proteomics analyses. There was a reduction in thickness of the molecular layer of cerebellar cortex in both exposure scenarios. Exposure to the POP mixture significantly affected expression of 65 of 13,800 transcripts, and 43 of 2,568 proteins, when compared to solvent control. PFOS alone affected
expression of 80 of 13,859 transcripts, and 69 of 2,555 proteins. Twenty-five genes and 15 proteins were common for both exposure groups. These findings point to alterations in molecular events linked to retinoid X receptor (RXR) signalling, neuronal cell proliferation and migration, cellular stress responses including unfolded protein response, lipid metabolism, and myelination. Exposure to the POP mixture increased methionine oxidation, whereas PFOS
decreased oxidation. Several of the altered genes and proteins are involved in a wide variety of neurological disorders. We conclude that POP exposure can interfere with fundamental aspects of neurodevelopment, altering molecular pathways that are associated with adverse neurocognitive and behavioural outcomes.
Original languageEnglish
JournalEnvironment International
Early online date27 Jun 2022
DOIs
Publication statusE-pub ahead of print - 27 Jun 2022

Keywords

  • Chicken embryo
  • Environmental toxicants
  • Neurodevelopment
  • Prenatal exposure
  • RNA-seq transcriptomics
  • Proteomics

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