Expression analysis of limb element markers during mouse embryonic development

Alexandra Rafipay; Amanda L. R. Berg; Lynda Erskine; Neil Vargesson

doi:10.1002/dvdy.24671

Expression analysis of limb element markers during mouse embryonic development

Alexandra Rafipay, Amanda L. R. Berg, Lynda Erskine, Neil Vargesson^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

8 Downloads (Pure)

Abstract

Background: While data regarding expression of limb element and tissue markers during normal mouse limb development exists, few studies show expression patterns in upper and lower limbs throughout key limb development stages. A comparison to normal developmental events is essential when analysing development of the limb in mutant mice models.
Results: Expression patterns of the joint marker Gdf5, tendon and ligament marker Scleraxis, early muscle marker MyoD1, and blood vessel marker Cadherin5 (Cdh5) are presented during the most active phases of embryonic mouse limb patterning. Anti-neurofilament staining of developing nerves in the fore- and hind-limbs and cartilage formation and progression also is described.
Conclusions: This study demonstrates and describes a range of key morphological markers, and methods, which together, can be used to assess normal and abnormal limb development.

Original language	English
Pages (from-to)	1217-1226
Number of pages	10
Journal	Developmental Dynamics
Volume	247
Issue number	11
Early online date	7 Nov 2018
DOIs	https://doi.org/10.1002/dvdy.24671
Publication status	Published - 20 Nov 2018

Keywords

gene expression
limb development
Forelimb
hindlimb
cartilage
nerves
alcian blue/alizarin red

Access to Document

10.1002/dvdy.24671Licence: CC BY

Expression analysis of limb element markers during mouse embryonic development
© 2018 The Authors. Developmental Dynamics published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/
Final published version, 31.1 MBLicence: CC BY

Cite this

@article{60514f74a1d34aee9c906534475e19d9,

title = "Expression analysis of limb element markers during mouse embryonic development",

abstract = "Background: While data regarding expression of limb element and tissue markers during normal mouse limb development exists, few studies show expression patterns in upper and lower limbs throughout key limb development stages. A comparison to normal developmental events is essential when analysing development of the limb in mutant mice models.Results: Expression patterns of the joint marker Gdf5, tendon and ligament marker Scleraxis, early muscle marker MyoD1, and blood vessel marker Cadherin5 (Cdh5) are presented during the most active phases of embryonic mouse limb patterning. Anti-neurofilament staining of developing nerves in the fore- and hind-limbs and cartilage formation and progression also is described.Conclusions: This study demonstrates and describes a range of key morphological markers, and methods, which together, can be used to assess normal and abnormal limb development. ",

keywords = "gene expression, limb development, Forelimb, hindlimb, cartilage, nerves, alcian blue/alizarin red",

author = "Alexandra Rafipay and Berg, {Amanda L. R.} and Lynda Erskine and Neil Vargesson",

note = "The authors declare no conflicts of interest. The authors thank Yolanda Ramirez Casas and J. Martin Collinson for technical advice and past and present lab staff for helpful discussions. ",

year = "2018",

month = nov,

day = "20",

doi = "10.1002/dvdy.24671",

language = "English",

volume = "247",

pages = "1217--1226",

journal = "Developmental Dynamics",

issn = "1058-8388",

publisher = "Wiley-Liss Inc.",

number = "11",

}

TY - JOUR

T1 - Expression analysis of limb element markers during mouse embryonic development

AU - Rafipay, Alexandra

AU - Berg, Amanda L. R.

AU - Erskine, Lynda

AU - Vargesson, Neil

N1 - The authors declare no conflicts of interest. The authors thank Yolanda Ramirez Casas and J. Martin Collinson for technical advice and past and present lab staff for helpful discussions.

PY - 2018/11/20

Y1 - 2018/11/20

N2 - Background: While data regarding expression of limb element and tissue markers during normal mouse limb development exists, few studies show expression patterns in upper and lower limbs throughout key limb development stages. A comparison to normal developmental events is essential when analysing development of the limb in mutant mice models.Results: Expression patterns of the joint marker Gdf5, tendon and ligament marker Scleraxis, early muscle marker MyoD1, and blood vessel marker Cadherin5 (Cdh5) are presented during the most active phases of embryonic mouse limb patterning. Anti-neurofilament staining of developing nerves in the fore- and hind-limbs and cartilage formation and progression also is described.Conclusions: This study demonstrates and describes a range of key morphological markers, and methods, which together, can be used to assess normal and abnormal limb development.

AB - Background: While data regarding expression of limb element and tissue markers during normal mouse limb development exists, few studies show expression patterns in upper and lower limbs throughout key limb development stages. A comparison to normal developmental events is essential when analysing development of the limb in mutant mice models.Results: Expression patterns of the joint marker Gdf5, tendon and ligament marker Scleraxis, early muscle marker MyoD1, and blood vessel marker Cadherin5 (Cdh5) are presented during the most active phases of embryonic mouse limb patterning. Anti-neurofilament staining of developing nerves in the fore- and hind-limbs and cartilage formation and progression also is described.Conclusions: This study demonstrates and describes a range of key morphological markers, and methods, which together, can be used to assess normal and abnormal limb development.

KW - gene expression

KW - limb development

KW - Forelimb

KW - hindlimb

KW - cartilage

KW - nerves

KW - alcian blue/alizarin red

U2 - 10.1002/dvdy.24671

DO - 10.1002/dvdy.24671

M3 - Article

VL - 247

SP - 1217

EP - 1226

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 11

ER -

Expression analysis of limb element markers during mouse embryonic development

Abstract

Keywords

Access to Document

Fingerprint

Cite this