Expression and distribution of InsP(3) receptor subtypes in proliferating vascular smooth muscle cells

Paul Nicholas Tasker, Chris Taylor, G F Nixon

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The expression and distribution of types 1, 2, and 3 inositol 1,4,5-trisphosphate receptor (InsP(3)R) in proliferating, primary cultures of rat aortic smooth muscle were compared to fully developed and differentiated rat aortic smooth muscle. Subtype-specific InsP(3)R antibodies revealed that the expression of type 1 InsP(3)R was similar in cultured aortic cells and aorta homogenate but expression of type 2 and 3 InsP(3)R subtypes was increased 3-fold in cultured aortic cells. The distribution of the type 1 InsP(3)R was located throughout the cytoplasm; type 2 InsP(3)R was found closely associated with the nucleus and at the plasma membrane; type 3 InsP(3)R was distributed predominantly around the nucleus. Alterations in InsP(3)R subtype expression and localization may have important functions in regulating intracellular calcium release around the nucleus when vascular smooth muscle cells switch to a more proliferating phenotype. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)907-912
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume273
Publication statusPublished - 2000

Keywords

  • smooth muscle
  • vascular
  • inositol 1,4,5-trisphosphate receptors
  • INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS
  • TRISPHOSPHATE RECEPTORS
  • SARCOPLASMIC-RETICULUM
  • INTRACELLULAR CA2+
  • NUCLEAR-ENVELOPE
  • GENE-EXPRESSION
  • TYPE-1
  • ATHEROSCLEROSIS
  • LOCALIZATION
  • INCREASE

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