Expression and distribution of InsP(3) receptor subtypes in proliferating vascular smooth muscle cells

Paul Nicholas Tasker, Chris Taylor, G F Nixon

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The expression and distribution of types 1, 2, and 3 inositol 1,4,5-trisphosphate receptor (InsP(3)R) in proliferating, primary cultures of rat aortic smooth muscle were compared to fully developed and differentiated rat aortic smooth muscle. Subtype-specific InsP(3)R antibodies revealed that the expression of type 1 InsP(3)R was similar in cultured aortic cells and aorta homogenate but expression of type 2 and 3 InsP(3)R subtypes was increased 3-fold in cultured aortic cells. The distribution of the type 1 InsP(3)R was located throughout the cytoplasm; type 2 InsP(3)R was found closely associated with the nucleus and at the plasma membrane; type 3 InsP(3)R was distributed predominantly around the nucleus. Alterations in InsP(3)R subtype expression and localization may have important functions in regulating intracellular calcium release around the nucleus when vascular smooth muscle cells switch to a more proliferating phenotype. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)907-912
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume273
Publication statusPublished - 2000

Keywords

  • smooth muscle
  • vascular
  • inositol 1,4,5-trisphosphate receptors
  • INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS
  • TRISPHOSPHATE RECEPTORS
  • SARCOPLASMIC-RETICULUM
  • INTRACELLULAR CA2+
  • NUCLEAR-ENVELOPE
  • GENE-EXPRESSION
  • TYPE-1
  • ATHEROSCLEROSIS
  • LOCALIZATION
  • INCREASE

Cite this

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title = "Expression and distribution of InsP(3) receptor subtypes in proliferating vascular smooth muscle cells",
abstract = "The expression and distribution of types 1, 2, and 3 inositol 1,4,5-trisphosphate receptor (InsP(3)R) in proliferating, primary cultures of rat aortic smooth muscle were compared to fully developed and differentiated rat aortic smooth muscle. Subtype-specific InsP(3)R antibodies revealed that the expression of type 1 InsP(3)R was similar in cultured aortic cells and aorta homogenate but expression of type 2 and 3 InsP(3)R subtypes was increased 3-fold in cultured aortic cells. The distribution of the type 1 InsP(3)R was located throughout the cytoplasm; type 2 InsP(3)R was found closely associated with the nucleus and at the plasma membrane; type 3 InsP(3)R was distributed predominantly around the nucleus. Alterations in InsP(3)R subtype expression and localization may have important functions in regulating intracellular calcium release around the nucleus when vascular smooth muscle cells switch to a more proliferating phenotype. (C) 2000 Academic Press.",
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journal = "Biochemical and Biophysical Research Communications",
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TY - JOUR

T1 - Expression and distribution of InsP(3) receptor subtypes in proliferating vascular smooth muscle cells

AU - Tasker, Paul Nicholas

AU - Taylor, Chris

AU - Nixon, G F

PY - 2000

Y1 - 2000

N2 - The expression and distribution of types 1, 2, and 3 inositol 1,4,5-trisphosphate receptor (InsP(3)R) in proliferating, primary cultures of rat aortic smooth muscle were compared to fully developed and differentiated rat aortic smooth muscle. Subtype-specific InsP(3)R antibodies revealed that the expression of type 1 InsP(3)R was similar in cultured aortic cells and aorta homogenate but expression of type 2 and 3 InsP(3)R subtypes was increased 3-fold in cultured aortic cells. The distribution of the type 1 InsP(3)R was located throughout the cytoplasm; type 2 InsP(3)R was found closely associated with the nucleus and at the plasma membrane; type 3 InsP(3)R was distributed predominantly around the nucleus. Alterations in InsP(3)R subtype expression and localization may have important functions in regulating intracellular calcium release around the nucleus when vascular smooth muscle cells switch to a more proliferating phenotype. (C) 2000 Academic Press.

AB - The expression and distribution of types 1, 2, and 3 inositol 1,4,5-trisphosphate receptor (InsP(3)R) in proliferating, primary cultures of rat aortic smooth muscle were compared to fully developed and differentiated rat aortic smooth muscle. Subtype-specific InsP(3)R antibodies revealed that the expression of type 1 InsP(3)R was similar in cultured aortic cells and aorta homogenate but expression of type 2 and 3 InsP(3)R subtypes was increased 3-fold in cultured aortic cells. The distribution of the type 1 InsP(3)R was located throughout the cytoplasm; type 2 InsP(3)R was found closely associated with the nucleus and at the plasma membrane; type 3 InsP(3)R was distributed predominantly around the nucleus. Alterations in InsP(3)R subtype expression and localization may have important functions in regulating intracellular calcium release around the nucleus when vascular smooth muscle cells switch to a more proliferating phenotype. (C) 2000 Academic Press.

KW - smooth muscle

KW - vascular

KW - inositol 1,4,5-trisphosphate receptors

KW - INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS

KW - TRISPHOSPHATE RECEPTORS

KW - SARCOPLASMIC-RETICULUM

KW - INTRACELLULAR CA2+

KW - NUCLEAR-ENVELOPE

KW - GENE-EXPRESSION

KW - TYPE-1

KW - ATHEROSCLEROSIS

KW - LOCALIZATION

KW - INCREASE

M3 - Article

VL - 273

SP - 907

EP - 912

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

ER -