Expression and purification strategies for the production of single-chain antibody and T-cell receptor fragments in E. coli

Peter E. Molloy, Barbara M. Graham, Pauline M. Cupit, Steven D. Grant, Andrew J R Porter, Charles Cunningham*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be denatured and refolded before the fragments attain their proper conformation.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalMolecular Biotechnology
Volume4
Issue number3
DOIs
Publication statusPublished - 1 Dec 1995

Keywords

  • Antibody fragments
  • Bacterial expression systems
  • Immunoglobulins
  • Metal chelate affinity chromatography
  • scAb
  • scTCR
  • T-cell receptors

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