Expression kinetics of interferon and interferon-induced genes in Atlantic salmon (Salmo salar) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia virus

Alastair James McBeath, M. Snow, C. J. Secombes, A. E. Ellis, Bertrand Collet

Research output: Contribution to journalArticle

Abstract

Infectious pancreatic necrosis virus (IPNV) and infectious salmon anaemia virus (ISAV) are economically important pathogens of the salmonid aquaculture industry. Atlantic salmon were challenged by intraperitoneal injection (i.p.) with either virus followed by time-course sampling. Cohabiting fish in the IPNV challenge were also sampled. Kidney tissue was analysed using a TaqMan (R) real-time PCR assay to measure the expression of a range of host immune genes in relation to the endogenous control, elongation factor 1 alpha (ELF). Host genes measured included Mx, type I and type II interferon (IFN), gamma IFN induced protein (gamma IP), interleukin-1 beta (IL-1 beta) and tumour necrosis factor alpha (TNF-alpha). Viral levels were also measured. In i.p. injected fish, both viruses greatly induced expression of Mx, gamma IP, type I and type II IFN by day 6 post-infection, however only ISAV caused substantial mortality. Some differences between the expression kinetics produced by both viruses were noted. Infection with ISAV increased IL-1 beta expression following day 6, but no effect was seen in fish infected with IPNV Neither virus induced TNF-alpha expression. This study confirms the presence of both type I and type II IFN responses and their induced genes in Atlantic salmon upon infection with an orthomyxovirus and a birnavirus. (c) 2006 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)230-241
Number of pages12
JournalFish & Shellfish Immunology
Volume22
Issue number3
Early online date23 May 2006
DOIs
Publication statusPublished - Mar 2007

Keywords

  • infectious pancreatic necrosis virus
  • infectious salmon anaemia virus
  • Atlantic salmon
  • interferon gene expression
  • real-time PCR
  • trout oncorhynchus-mykiss
  • double-stranded-RNA
  • viral hemorrhagic septicemia
  • poly I-C
  • antiviral activity
  • human macrophages
  • gamma production
  • MX proteins
  • IFN-gamma
  • cells

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