Expression of a cellulase gene, celA, from the rumen fungus Neocallimastix patriciarum in Streptococcus bovis by means of promoter fusions

M S Ekinci, J C Martin, H J Flint

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Abstract

The aerotolerant rumen bacterium, Streptococcus bovis, has been used as a host for expression of genes of eukaryotic origin. The coding regions of the celA cellulase gene from the rumen fungus, Neocallimastix patriciarum, were fused with bacterial promoter/signal peptide regions from the Ruminococcus flavefaciens xynD and S. bovis beta-(1,3-1,4)-glucanase genes. Fusion cassettes were built into shuttle vector constructs based on pIL253 or pTRW10 and constructs carrying celA were transformed into S. bovis JB1. Active N. patriciarum cellulase was produced in S. bovis with either promoter, although better expression levels were obtained with the native S. bovis beta-glucanase promoter fragment.

Original languageEnglish
Pages (from-to)735-741
Number of pages7
JournalBiotechnology Letters
Volume24
Issue number9
Publication statusPublished - May 2002

Keywords

  • cellulase
  • Neocallimastix patriciarum
  • promoter fusion
  • Streptococcus bovis JB1
  • BACTERIA
  • CLONING
  • JB1

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