Expression of Bcl-2 and its homologues in human eosinophils - Modulation by interleukin-5

G Dewson, Garry Walsh, A J Wardlaw

Research output: Contribution to journalArticle

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Abstract

The Bcl-2 family has been shown to be vital regulators of programmed cell death in numerous systems. To investigate the role of such proteins in the regulation of apoptosis of eosinophils, the expression of Bcl-2 and homologues Bcl-x(L), (death antagonists), Bax, and Bcl-x(S) (death agonists) were examined by immunoblot, flow cytometry, and reverse transcriptase-polymerase chain reaction analysis, Potential modulation of apoptosis-associated molecules during spontaneous apoptosis and in the presence of interleukin (IL)-5 was also investigated. Peripheral blood eosinophils were found to express constitutively Pax and Bcl-x, but Bcl-2 was absent. Analysis of mRNA revealed that the bcl-x(L), isoform predominated, although bcl-x(S), was also detectable. Spontaneous apoptosis due to culturing in the absence of cytokines for 24 h did not result in modulation of any of the Bcl-2 homologues examined. Culturing eosinophils in the presence of 100 pg/ml IL-5 for 24 h significantly reduced apoptosis (P < 0.01) to 10.7 +/- 2.6% compared with 46.8 +/- 7.4% in the absence of IL-5, and induced Bcl-2 mRNA and protein expression, with no detectable change in Bax, Bcl-x, or beta-actin as a control. This investigation indicates a specific profile of apoptotic molecules in eosinophils distinct from that of neutrophils, and indicates that survival-enhancing IL-5 modulates the expression of Bcl-2 in vitro.

Original languageEnglish
Pages (from-to)720-728
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume20
Issue number4
Publication statusPublished - Apr 1999

Keywords

  • PROGRAMMED CELL-DEATH
  • COLONY-STIMULATING FACTOR
  • HUMAN FOLLICULAR LYMPHOMA
  • HUMAN PERIPHERAL-BLOOD
  • CYTOCHROME-C
  • GRANULOCYTE APOPTOSIS
  • SURVIVAL
  • PROTEIN
  • BAX
  • BCL-X(L)

Cite this

Expression of Bcl-2 and its homologues in human eosinophils - Modulation by interleukin-5. / Dewson, G ; Walsh, Garry; Wardlaw, A J .

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 20, No. 4, 04.1999, p. 720-728.

Research output: Contribution to journalArticle

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N2 - The Bcl-2 family has been shown to be vital regulators of programmed cell death in numerous systems. To investigate the role of such proteins in the regulation of apoptosis of eosinophils, the expression of Bcl-2 and homologues Bcl-x(L), (death antagonists), Bax, and Bcl-x(S) (death agonists) were examined by immunoblot, flow cytometry, and reverse transcriptase-polymerase chain reaction analysis, Potential modulation of apoptosis-associated molecules during spontaneous apoptosis and in the presence of interleukin (IL)-5 was also investigated. Peripheral blood eosinophils were found to express constitutively Pax and Bcl-x, but Bcl-2 was absent. Analysis of mRNA revealed that the bcl-x(L), isoform predominated, although bcl-x(S), was also detectable. Spontaneous apoptosis due to culturing in the absence of cytokines for 24 h did not result in modulation of any of the Bcl-2 homologues examined. Culturing eosinophils in the presence of 100 pg/ml IL-5 for 24 h significantly reduced apoptosis (P < 0.01) to 10.7 +/- 2.6% compared with 46.8 +/- 7.4% in the absence of IL-5, and induced Bcl-2 mRNA and protein expression, with no detectable change in Bax, Bcl-x, or beta-actin as a control. This investigation indicates a specific profile of apoptotic molecules in eosinophils distinct from that of neutrophils, and indicates that survival-enhancing IL-5 modulates the expression of Bcl-2 in vitro.

AB - The Bcl-2 family has been shown to be vital regulators of programmed cell death in numerous systems. To investigate the role of such proteins in the regulation of apoptosis of eosinophils, the expression of Bcl-2 and homologues Bcl-x(L), (death antagonists), Bax, and Bcl-x(S) (death agonists) were examined by immunoblot, flow cytometry, and reverse transcriptase-polymerase chain reaction analysis, Potential modulation of apoptosis-associated molecules during spontaneous apoptosis and in the presence of interleukin (IL)-5 was also investigated. Peripheral blood eosinophils were found to express constitutively Pax and Bcl-x, but Bcl-2 was absent. Analysis of mRNA revealed that the bcl-x(L), isoform predominated, although bcl-x(S), was also detectable. Spontaneous apoptosis due to culturing in the absence of cytokines for 24 h did not result in modulation of any of the Bcl-2 homologues examined. Culturing eosinophils in the presence of 100 pg/ml IL-5 for 24 h significantly reduced apoptosis (P < 0.01) to 10.7 +/- 2.6% compared with 46.8 +/- 7.4% in the absence of IL-5, and induced Bcl-2 mRNA and protein expression, with no detectable change in Bax, Bcl-x, or beta-actin as a control. This investigation indicates a specific profile of apoptotic molecules in eosinophils distinct from that of neutrophils, and indicates that survival-enhancing IL-5 modulates the expression of Bcl-2 in vitro.

KW - PROGRAMMED CELL-DEATH

KW - COLONY-STIMULATING FACTOR

KW - HUMAN FOLLICULAR LYMPHOMA

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KW - CYTOCHROME-C

KW - GRANULOCYTE APOPTOSIS

KW - SURVIVAL

KW - PROTEIN

KW - BAX

KW - BCL-X(L)

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JO - American Journal of Respiratory Cell and Molecular Biology

JF - American Journal of Respiratory Cell and Molecular Biology

SN - 1044-1549

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