Expression of beta 1 integrins in IgA nephropathy

G S Hillis, P RoyChaudhury, L A Duthie, Keith Nicol Stewart, P A J Brown, A M MacLeod

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aim. To compare the expression of beta 1 integrins in renal biopsies from pati

Methods. Thirty renal biopsies from patients with IgA disease plus six control specimens were stained with monoclonal antibodies directed against the alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha v, and beta 1 integrin chains using the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. The intensity of integrin expression was graded semiquantitatively by a pathologist unaware of the antibody used.

Results. Glomerular crescents stained strongly for alpha 3, alpha v, and beta 1, but integrin expression was greatly reduced or absent in fibrotic glomeruli. There were no alterations in the intensity of mesangial cell staining for any of the integrins tested. There was accentuated staining for the alpha 2, alpha 5, alpha v, and <beta1r chains in areas of interstitial scarring plus alpha 2, alpha 3, alpha v, and beta 1 on damaged tubules. Inflammatory cells expressed alpha 4, alpha 5, and beta 1.

Conclusions. In IgA nephropathy the interstitium is the main site of altered beta 1 integrin expression. Glomerular crescents also express several beta 1 integrins, but we found no differences in the intensity of integrin expression on mesangial cells. Altered beta 1 integrin expression may play a role in tubulointerstitial scarring in IgA disease. Thus modulation of integrin expression might attenuate this process.

Original languageEnglish
Pages (from-to)1137-1142
Number of pages6
JournalNephrology Dialysis Transplantation
Volume12
Issue number6
Publication statusPublished - Jun 1997

Keywords

  • beta 1 integrins
  • glomerulonephritis
  • IgA nephropathy
  • fibrosis
  • EXTRACELLULAR-MATRIX COMPONENTS
  • MESANGIAL CELLS
  • HUMAN-KIDNEY
  • MONOCLONAL-ANTIBODIES
  • GLOMERULONEPHRITIS
  • RECEPTORS
  • PROTEINS
  • COLLAGEN
  • FIBROSIS
  • b1 integrins
  • IgA neuropathy

Cite this

Hillis, G. S., RoyChaudhury, P., Duthie, L. A., Stewart, K. N., Brown, P. A. J., & MacLeod, A. M. (1997). Expression of beta 1 integrins in IgA nephropathy. Nephrology Dialysis Transplantation, 12(6), 1137-1142.

Expression of beta 1 integrins in IgA nephropathy. / Hillis, G S ; RoyChaudhury, P ; Duthie, L A ; Stewart, Keith Nicol; Brown, P A J ; MacLeod, A M .

In: Nephrology Dialysis Transplantation, Vol. 12, No. 6, 06.1997, p. 1137-1142.

Research output: Contribution to journalArticle

Hillis, GS, RoyChaudhury, P, Duthie, LA, Stewart, KN, Brown, PAJ & MacLeod, AM 1997, 'Expression of beta 1 integrins in IgA nephropathy', Nephrology Dialysis Transplantation, vol. 12, no. 6, pp. 1137-1142.
Hillis GS, RoyChaudhury P, Duthie LA, Stewart KN, Brown PAJ, MacLeod AM. Expression of beta 1 integrins in IgA nephropathy. Nephrology Dialysis Transplantation. 1997 Jun;12(6):1137-1142.
Hillis, G S ; RoyChaudhury, P ; Duthie, L A ; Stewart, Keith Nicol ; Brown, P A J ; MacLeod, A M . / Expression of beta 1 integrins in IgA nephropathy. In: Nephrology Dialysis Transplantation. 1997 ; Vol. 12, No. 6. pp. 1137-1142.
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abstract = "Aim. To compare the expression of beta 1 integrins in renal biopsies from patiMethods. Thirty renal biopsies from patients with IgA disease plus six control specimens were stained with monoclonal antibodies directed against the alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha v, and beta 1 integrin chains using the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. The intensity of integrin expression was graded semiquantitatively by a pathologist unaware of the antibody used.Results. Glomerular crescents stained strongly for alpha 3, alpha v, and beta 1, but integrin expression was greatly reduced or absent in fibrotic glomeruli. There were no alterations in the intensity of mesangial cell staining for any of the integrins tested. There was accentuated staining for the alpha 2, alpha 5, alpha v, and Conclusions. In IgA nephropathy the interstitium is the main site of altered beta 1 integrin expression. Glomerular crescents also express several beta 1 integrins, but we found no differences in the intensity of integrin expression on mesangial cells. Altered beta 1 integrin expression may play a role in tubulointerstitial scarring in IgA disease. Thus modulation of integrin expression might attenuate this process.",
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AU - Hillis, G S

AU - RoyChaudhury, P

AU - Duthie, L A

AU - Stewart, Keith Nicol

AU - Brown, P A J

AU - MacLeod, A M

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N2 - Aim. To compare the expression of beta 1 integrins in renal biopsies from patiMethods. Thirty renal biopsies from patients with IgA disease plus six control specimens were stained with monoclonal antibodies directed against the alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha v, and beta 1 integrin chains using the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. The intensity of integrin expression was graded semiquantitatively by a pathologist unaware of the antibody used.Results. Glomerular crescents stained strongly for alpha 3, alpha v, and beta 1, but integrin expression was greatly reduced or absent in fibrotic glomeruli. There were no alterations in the intensity of mesangial cell staining for any of the integrins tested. There was accentuated staining for the alpha 2, alpha 5, alpha v, and Conclusions. In IgA nephropathy the interstitium is the main site of altered beta 1 integrin expression. Glomerular crescents also express several beta 1 integrins, but we found no differences in the intensity of integrin expression on mesangial cells. Altered beta 1 integrin expression may play a role in tubulointerstitial scarring in IgA disease. Thus modulation of integrin expression might attenuate this process.

AB - Aim. To compare the expression of beta 1 integrins in renal biopsies from patiMethods. Thirty renal biopsies from patients with IgA disease plus six control specimens were stained with monoclonal antibodies directed against the alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha v, and beta 1 integrin chains using the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. The intensity of integrin expression was graded semiquantitatively by a pathologist unaware of the antibody used.Results. Glomerular crescents stained strongly for alpha 3, alpha v, and beta 1, but integrin expression was greatly reduced or absent in fibrotic glomeruli. There were no alterations in the intensity of mesangial cell staining for any of the integrins tested. There was accentuated staining for the alpha 2, alpha 5, alpha v, and Conclusions. In IgA nephropathy the interstitium is the main site of altered beta 1 integrin expression. Glomerular crescents also express several beta 1 integrins, but we found no differences in the intensity of integrin expression on mesangial cells. Altered beta 1 integrin expression may play a role in tubulointerstitial scarring in IgA disease. Thus modulation of integrin expression might attenuate this process.

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KW - MONOCLONAL-ANTIBODIES

KW - GLOMERULONEPHRITIS

KW - RECEPTORS

KW - PROTEINS

KW - COLLAGEN

KW - FIBROSIS

KW - b1 integrins

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