Expression of bifunctional genes encoding xylanase and beta(1,3-1,4)-glucanase in Gram-positive bacteria

M S Ekinci, H J Flint

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The xylanase and beta (1,3-1,4)-Glucanase activities encoding gene from the cellulolytic rumen anaerobe bacterium was cloned into Escherichia/Streptococcus shuttle vector for introduction into Gram-positive bacteria, including the rumen facultative anaerobe bacterium Streptococcus bovis. Activities due to the cloned gene decreased in the stationary phase in batch cultures of S. bovis, reflecting the sensitivity of the cloned enzymes to inactivation in the presence of accumulated lactic acid. Cloned gene activity was detected in the culture supernatant, indicating recognition of the cloned gene signal peptide by Gram-positive bacteria.

Original languageEnglish
Pages (from-to)771-775
Number of pages5
JournalTurkish Journal of Veterinary & Animal Sciences
Volume25
Issue number5
Publication statusPublished - 2001

Keywords

  • gene expression
  • Gram-positive bacteria
  • enzyme
  • STREPTOCOCCUS-BOVIS JB1
  • RUMINOCOCCUS-FLAVEFACIENS
  • ESCHERICHIA-COLI
  • SANGUIS
  • VECTOR
  • RUMEN
  • TRANSFORMATION
  • CONSTRUCTION
  • DOMAINS
  • CLONING

Cite this

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title = "Expression of bifunctional genes encoding xylanase and beta(1,3-1,4)-glucanase in Gram-positive bacteria",
abstract = "The xylanase and beta (1,3-1,4)-Glucanase activities encoding gene from the cellulolytic rumen anaerobe bacterium was cloned into Escherichia/Streptococcus shuttle vector for introduction into Gram-positive bacteria, including the rumen facultative anaerobe bacterium Streptococcus bovis. Activities due to the cloned gene decreased in the stationary phase in batch cultures of S. bovis, reflecting the sensitivity of the cloned enzymes to inactivation in the presence of accumulated lactic acid. Cloned gene activity was detected in the culture supernatant, indicating recognition of the cloned gene signal peptide by Gram-positive bacteria.",
keywords = "gene expression, Gram-positive bacteria, enzyme, STREPTOCOCCUS-BOVIS JB1, RUMINOCOCCUS-FLAVEFACIENS, ESCHERICHIA-COLI, SANGUIS, VECTOR, RUMEN, TRANSFORMATION, CONSTRUCTION, DOMAINS, CLONING",
author = "Ekinci, {M S} and Flint, {H J}",
year = "2001",
language = "English",
volume = "25",
pages = "771--775",
journal = "Turkish Journal of Veterinary & Animal Sciences",
issn = "1300-0128",
publisher = "TUBITAK",
number = "5",

}

TY - JOUR

T1 - Expression of bifunctional genes encoding xylanase and beta(1,3-1,4)-glucanase in Gram-positive bacteria

AU - Ekinci, M S

AU - Flint, H J

PY - 2001

Y1 - 2001

N2 - The xylanase and beta (1,3-1,4)-Glucanase activities encoding gene from the cellulolytic rumen anaerobe bacterium was cloned into Escherichia/Streptococcus shuttle vector for introduction into Gram-positive bacteria, including the rumen facultative anaerobe bacterium Streptococcus bovis. Activities due to the cloned gene decreased in the stationary phase in batch cultures of S. bovis, reflecting the sensitivity of the cloned enzymes to inactivation in the presence of accumulated lactic acid. Cloned gene activity was detected in the culture supernatant, indicating recognition of the cloned gene signal peptide by Gram-positive bacteria.

AB - The xylanase and beta (1,3-1,4)-Glucanase activities encoding gene from the cellulolytic rumen anaerobe bacterium was cloned into Escherichia/Streptococcus shuttle vector for introduction into Gram-positive bacteria, including the rumen facultative anaerobe bacterium Streptococcus bovis. Activities due to the cloned gene decreased in the stationary phase in batch cultures of S. bovis, reflecting the sensitivity of the cloned enzymes to inactivation in the presence of accumulated lactic acid. Cloned gene activity was detected in the culture supernatant, indicating recognition of the cloned gene signal peptide by Gram-positive bacteria.

KW - gene expression

KW - Gram-positive bacteria

KW - enzyme

KW - STREPTOCOCCUS-BOVIS JB1

KW - RUMINOCOCCUS-FLAVEFACIENS

KW - ESCHERICHIA-COLI

KW - SANGUIS

KW - VECTOR

KW - RUMEN

KW - TRANSFORMATION

KW - CONSTRUCTION

KW - DOMAINS

KW - CLONING

M3 - Article

VL - 25

SP - 771

EP - 775

JO - Turkish Journal of Veterinary & Animal Sciences

JF - Turkish Journal of Veterinary & Animal Sciences

SN - 1300-0128

IS - 5

ER -