Expression of CD44v6 but not E-cadherin or beta-catenin influences prognosis in primary pulmonary adenocarcinoma

S Ramasami, K M Kerr, A D Chapman, George King, J S Cockburn, R R Jeffrey

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    Primary pulmonary adenocarcinoma was studied, looking for relationships between the expression of cell adhesion molecules (CAMs) E-cadherin, beta -catenin and CD44v6, and clinicopathological tumour parameters and patient post-operative survival. Formalin-fixed, paraffin-embedded tissue from 120 primary lung adenocarcinomas, including 23 poorly differentiated tumours, 17 of probable bronchial origin, and 29 with a prominent bronchioloalveolar pattern, together with nodal metastatic tumour from 34 of these patients was stained using monoclonal antibodies and immunohistochemistry. Sections were scored either high level (>10% cells positive) or low level (<10% positive). High level expression of CD44v6 was retained in 28.4% (34/120) of tumours, while high levels of E-cadherin (57.5%, 69/120) and <beta>-catenin (80.8%, 97/120) were more frequent. For all CAMs, staining levels did not correlate with nodal status, stage or tumour type. The apical or basal staining seen in normal bronchial and alveolar epithelium was often seen in papillary, glandular, and bronchioloalveolar areas of tumour, while solid invasive tumour more often showed pericellular staining. When the staining for each CAM in 34 nodal metastases was compared with that in the corresponding primary tumour, a high degree of concordance was found, with no tendency for metastases to show less staining than the primary tumour. Expression of E-cadherin and beta -catenin in the primary tumour had no influence on post-operative survival, but patients whose tumours had low level CD44v6 expression had a poorer post-operative survival than those with high levels of CD44v6 (p = 0.0014 for all patients, p = 0.0012 for stage I patients only). In primary pulmonary adenocarcinoma, the levels of expression of E-cadherin, beta -catenin, and CD44v6 are not associated with lymph node metastases or tumour stage but the staining pattern is associated with tumour morphology. Low levels of CD44v6 expression predict a poor post-operative survival, independently of stage, while there is no such relationship with the expression of E-cadherin or beta -catenin. Copyright (C) 2000 John Wiley & Sons, Ltd.

    Original languageEnglish
    Pages (from-to)427-432
    Number of pages6
    JournalThe Journal of pathology
    Volume192
    Publication statusPublished - 2000

    Keywords

    • lung cancer
    • adenocarcinoma
    • E-cadherin
    • beta-catenin
    • CD44v6
    • immunohistochemistry
    • prognosis
    • CELL LUNG-CANCER
    • BREAST CARCINOGENESIS
    • TUMOR PROGRESSION
    • ADHESION
    • VARIANT
    • COMPLEX
    • CARCINOMAS
    • STANDARD
    • ISOFORMS
    • SYSTEM

    Cite this

    Expression of CD44v6 but not E-cadherin or beta-catenin influences prognosis in primary pulmonary adenocarcinoma. / Ramasami, S ; Kerr, K M ; Chapman, A D ; King, George; Cockburn, J S ; Jeffrey, R R .

    In: The Journal of pathology, Vol. 192, 2000, p. 427-432.

    Research output: Contribution to journalArticle

    Ramasami, S ; Kerr, K M ; Chapman, A D ; King, George ; Cockburn, J S ; Jeffrey, R R . / Expression of CD44v6 but not E-cadherin or beta-catenin influences prognosis in primary pulmonary adenocarcinoma. In: The Journal of pathology. 2000 ; Vol. 192. pp. 427-432.
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    TY - JOUR

    T1 - Expression of CD44v6 but not E-cadherin or beta-catenin influences prognosis in primary pulmonary adenocarcinoma

    AU - Ramasami, S

    AU - Kerr, K M

    AU - Chapman, A D

    AU - King, George

    AU - Cockburn, J S

    AU - Jeffrey, R R

    PY - 2000

    Y1 - 2000

    N2 - Primary pulmonary adenocarcinoma was studied, looking for relationships between the expression of cell adhesion molecules (CAMs) E-cadherin, beta -catenin and CD44v6, and clinicopathological tumour parameters and patient post-operative survival. Formalin-fixed, paraffin-embedded tissue from 120 primary lung adenocarcinomas, including 23 poorly differentiated tumours, 17 of probable bronchial origin, and 29 with a prominent bronchioloalveolar pattern, together with nodal metastatic tumour from 34 of these patients was stained using monoclonal antibodies and immunohistochemistry. Sections were scored either high level (>10% cells positive) or low level (<10% positive). High level expression of CD44v6 was retained in 28.4% (34/120) of tumours, while high levels of E-cadherin (57.5%, 69/120) and -catenin (80.8%, 97/120) were more frequent. For all CAMs, staining levels did not correlate with nodal status, stage or tumour type. The apical or basal staining seen in normal bronchial and alveolar epithelium was often seen in papillary, glandular, and bronchioloalveolar areas of tumour, while solid invasive tumour more often showed pericellular staining. When the staining for each CAM in 34 nodal metastases was compared with that in the corresponding primary tumour, a high degree of concordance was found, with no tendency for metastases to show less staining than the primary tumour. Expression of E-cadherin and beta -catenin in the primary tumour had no influence on post-operative survival, but patients whose tumours had low level CD44v6 expression had a poorer post-operative survival than those with high levels of CD44v6 (p = 0.0014 for all patients, p = 0.0012 for stage I patients only). In primary pulmonary adenocarcinoma, the levels of expression of E-cadherin, beta -catenin, and CD44v6 are not associated with lymph node metastases or tumour stage but the staining pattern is associated with tumour morphology. Low levels of CD44v6 expression predict a poor post-operative survival, independently of stage, while there is no such relationship with the expression of E-cadherin or beta -catenin. Copyright (C) 2000 John Wiley & Sons, Ltd.

    AB - Primary pulmonary adenocarcinoma was studied, looking for relationships between the expression of cell adhesion molecules (CAMs) E-cadherin, beta -catenin and CD44v6, and clinicopathological tumour parameters and patient post-operative survival. Formalin-fixed, paraffin-embedded tissue from 120 primary lung adenocarcinomas, including 23 poorly differentiated tumours, 17 of probable bronchial origin, and 29 with a prominent bronchioloalveolar pattern, together with nodal metastatic tumour from 34 of these patients was stained using monoclonal antibodies and immunohistochemistry. Sections were scored either high level (>10% cells positive) or low level (<10% positive). High level expression of CD44v6 was retained in 28.4% (34/120) of tumours, while high levels of E-cadherin (57.5%, 69/120) and -catenin (80.8%, 97/120) were more frequent. For all CAMs, staining levels did not correlate with nodal status, stage or tumour type. The apical or basal staining seen in normal bronchial and alveolar epithelium was often seen in papillary, glandular, and bronchioloalveolar areas of tumour, while solid invasive tumour more often showed pericellular staining. When the staining for each CAM in 34 nodal metastases was compared with that in the corresponding primary tumour, a high degree of concordance was found, with no tendency for metastases to show less staining than the primary tumour. Expression of E-cadherin and beta -catenin in the primary tumour had no influence on post-operative survival, but patients whose tumours had low level CD44v6 expression had a poorer post-operative survival than those with high levels of CD44v6 (p = 0.0014 for all patients, p = 0.0012 for stage I patients only). In primary pulmonary adenocarcinoma, the levels of expression of E-cadherin, beta -catenin, and CD44v6 are not associated with lymph node metastases or tumour stage but the staining pattern is associated with tumour morphology. Low levels of CD44v6 expression predict a poor post-operative survival, independently of stage, while there is no such relationship with the expression of E-cadherin or beta -catenin. Copyright (C) 2000 John Wiley & Sons, Ltd.

    KW - lung cancer

    KW - adenocarcinoma

    KW - E-cadherin

    KW - beta-catenin

    KW - CD44v6

    KW - immunohistochemistry

    KW - prognosis

    KW - CELL LUNG-CANCER

    KW - BREAST CARCINOGENESIS

    KW - TUMOR PROGRESSION

    KW - ADHESION

    KW - VARIANT

    KW - COMPLEX

    KW - CARCINOMAS

    KW - STANDARD

    KW - ISOFORMS

    KW - SYSTEM

    M3 - Article

    VL - 192

    SP - 427

    EP - 432

    JO - The Journal of pathology

    JF - The Journal of pathology

    SN - 0022-3417

    ER -